Rapid serological diagnosis systems for human leptospirosis screening in Cuba

human leptospirosis requires rapid and early microbiological diagnosis since it is a common lethal disease worldwide. to increase the quality of microbiological diagnosis of this infection, to expand the knowledge on the circulation of groups of leptospiras in Cuba and to show the benefits of an agg...

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Bibliographic Details
Published in:	Revista cubana de medicina tropical Vol. 63; no. 3; pp. 239 - 245
Main Authors:	Obregón Fuentes, C Ana Margarita, Fernandez Molina, Carmen, Martínez Motas, C Isabel, Llop Hernández, C Alina, Rodríguez González, Islay, Rodríguez Silveira, José, Rodríguez, Yaindrys, Valdés, Yanais
Format:	Journal Article
Language:	English Spanish
Published:	Cuba 01-09-2011
Subjects:	Cuba Humans Leptospirosis - blood Leptospirosis - diagnosis Serologic Tests - methods Time Factors Cuba
Online Access:	Get full text
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Summary:	human leptospirosis requires rapid and early microbiological diagnosis since it is a common lethal disease worldwide. to increase the quality of microbiological diagnosis of this infection, to expand the knowledge on the circulation of groups of leptospiras in Cuba and to show the benefits of an agglutination assay using Cuban latex particles and of commercial immunochromatogenic systems LEPTO Dipstick, Lepto Tek Lateral Flow, Lepto Tek Dri Dot and SD Leptospira IgM-IgG. this descriptive research used sera from positive and negative control cases to evaluate and measure the diagnostic value of rapid serological diagnosis systems with respect to the microagglutination method of reference (MAT). All the techniques used in this report are described in the Manual of Operations and Procedures of the Leptospira Lab in "Pedro Kourí" Institute of Tropical Medicine. all the studied diagnosis systems exhibited acceptable values of sensitivity, specificity and agreement when compared to the international microagglutination method of reference with live microorganisms. The great selectivity (antigen reactivity) and the diagnostic reliability of the diagnostic systems were confirmed; particularly the mixed Cuban-made latex, the LEPTO Dipstick and the SD Leptospira IgM-IgG. the procedures used in this research work exceeded the traditional methods including the microagglutination method of reference in terms of easiness, rapidity, technical simplicity and level of performance, and all were useful for the screening of antibodies to leptospiras.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0375-0760