An improved pig reference genome sequence to enable pig genetics and genomics research

Abstract Background The domestic pig (Sus scrofa) is important both as a food source and as a biomedical model given its similarity in size, anatomy, physiology, metabolism, pathology, and pharmacology to humans. The draft reference genome (Sscrofa10.2) of a purebred Duroc female pig established usi...

Full description

Saved in:

Bibliographic Details
Published in:	Gigascience Vol. 9; no. 6
Main Authors:	Warr, Amanda, Affara, Nabeel, Aken, Bronwen, Beiki, Hamid, Bickhart, Derek M, Billis, Konstantinos, Chow, William, Eory, Lel, Finlayson, Heather A, Flicek, Paul, Girón, Carlos G, Griffin, Darren K, Hall, Richard, Hannum, Greg, Hourlier, Thibaut, Howe, Kerstin, Hume, David A, Izuogu, Osagie, Kim, Kristi, Koren, Sergey, Liu, Haibou, Manchanda, Nancy, Martin, Fergal J, Nonneman, Dan J, O'Connor, Rebecca E, Phillippy, Adam M, Rohrer, Gary A, Rosen, Benjamin D, Rund, Laurie A, Sargent, Carole A, Schook, Lawrence B, Schroeder, Steven G, Schwartz, Ariel S, Skinner, Ben M, Talbot, Richard, Tseng, Elizabeth, Tuggle, Christopher K, Watson, Mick, Smith, Timothy P L, Archibald, Alan L
Format:	Journal Article
Language:	English
Published:	United States Oxford University Press 01-06-2020
Subjects:	Animals Annotations Assemblies Computational Biology - methods Females Food sources Genome Genomes Genomics Genomics - methods Molecular Sequence Annotation Nucleotide sequence Pharmacology Reproducibility of Results Sequence Analysis, DNA - methods Sequences Short range order Sus scrofa - immunology Swine reference assembly pig genomes genome annotation pig
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Abstract Background The domestic pig (Sus scrofa) is important both as a food source and as a biomedical model given its similarity in size, anatomy, physiology, metabolism, pathology, and pharmacology to humans. The draft reference genome (Sscrofa10.2) of a purebred Duroc female pig established using older clone-based sequencing methods was incomplete, and unresolved redundancies, short-range order and orientation errors, and associated misassembled genes limited its utility. Results We present 2 annotated highly contiguous chromosome-level genome assemblies created with more recent long-read technologies and a whole-genome shotgun strategy, 1 for the same Duroc female (Sscrofa11.1) and 1 for an outbred, composite-breed male (USMARCv1.0). Both assemblies are of substantially higher (>90-fold) continuity and accuracy than Sscrofa10.2. Conclusions These highly contiguous assemblies plus annotation of a further 11 short-read assemblies provide an unprecedented view of the genetic make-up of this important agricultural and biomedical model species. We propose that the improved Duroc assembly (Sscrofa11.1) become the reference genome for genomic research in pigs.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2047-217X 2047-217X
DOI:	10.1093/gigascience/giaa051