Dense Genetic Linkage Maps of Three Populus Species (Populus deltoides, P. nigra and P. trichocarpa) Based on AFLP and Microsatellite Markers

Dense Genetic Linkage Maps of Three Populus Species (Populus deltoides, P. nigra and P. trichocarpa) Based on AFLP and Microsatellite Markers

Populus deltoides, P. nigra, and P. trichocarpa are the most important species for poplar breeding programs worldwide. In addition, Populus has become a model for fundamental research on trees. Linkage maps were constructed for these three species by analyzing progeny of two controlled crosses shari...

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Bibliographic Details
Published in:Genetics (Austin) Vol. 158; no. 2; pp. 787 - 809
Main Authors: Cervera, Maria-Teresa, Storme, Veronique, Ivens, Bart, Gusmao, Jaqueline, Liu, Ben H, Hostyn, Vanessa, Van Slycken, Jos, Van Montagu, Marc, Boerjan, Wout
Format: Journal Article
Language:English
Published: United States Genetics Soc America 01-06-2001
Genetics Society of America
Subjects:
Chromosome Mapping
Cloning
Crosses, Genetic
DNA - metabolism
Genes, Plant
Genetic Linkage
Genetic Markers - genetics
Genetics
Heterozygote
Lumber industry
Microsatellite Repeats
Models, Genetic
Models, Statistical
Polymorphism, Genetic
Populus deltoides
Populus nigra
Populus trichocarpa
Sequence Tagged Sites
Trees
Trees - genetics
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Summary:Populus deltoides, P. nigra, and P. trichocarpa are the most important species for poplar breeding programs worldwide. In addition, Populus has become a model for fundamental research on trees. Linkage maps were constructed for these three species by analyzing progeny of two controlled crosses sharing the same female parent, Populus deltoides cv. S9-2 x P. nigra cv. Ghoy and P. deltoides cv. S9-2 x P. trichocarpa cv. V24. The two-way pseudotestcross mapping strategy was used to construct the maps. Amplified fragment length polymorphism (AFLP) markers that segregated 1:1 were used to form the four parental maps. Microsatellites and sequence-tagged sites were used to align homoeologous groups between the maps and to merge linkage groups within the individual maps. Linkage analysis and alignment of the homoeologous groups resulted in 566 markers distributed over 19 groups for P. deltoides covering 86% of the genome, 339 markers distributed over 19 groups for P. trichocarpa covering 73%, and 369 markers distributed over 28 groups for P. nigra covering 61%. Several tests for randomness showed that the AFLP markers were randomly distributed over the genome.
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ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/158.2.787