Search Results - "Little, Shawn C."

Precise Developmental Gene Expression Arises from Globally Stochastic Transcriptional Activity by Little, Shawn C., Tikhonov, Mikhail, Gregor, Thomas

“…Early embryonic patterning events are strikingly precise, a fact that appears incompatible with the stochastic gene expression observed across phyla. Using…”
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Diverse Spatial Expression Patterns Emerge from Unified Kinetics of Transcriptional Bursting by Zoller, Benjamin, Little, Shawn C., Gregor, Thomas

“…How transcriptional bursting relates to gene regulation is a central question that has persisted for more than a decade. Here, we measure nascent…”
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Spatiotemporal Patterning of Zygotic Genome Activation in a Model Vertebrate Embryo by Chen, Hui, Einstein, Lily C., Little, Shawn C., Good, Matthew C.

“…A defining feature of early embryogenesis is the transition from maternal to zygotic control. This transition requires embryo-wide zygotic genome activation…”
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The formation of the Bicoid morphogen gradient requires protein movement from anteriorly localized mRNA by Little, Shawn C, Tkačik, Gašper, Kneeland, Thomas B, Wieschaus, Eric F, Gregor, Thomas

“…The Bicoid morphogen gradient directs the patterning of cell fates along the anterior-posterior axis of the syncytial Drosophila embryo and serves as a…”
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Chromatin targeting of nuclear pore proteins induces chromatin decondensation by Kuhn, Terra M, Pascual-Garcia, Pau, Gozalo, Alejandro, Little, Shawn C, Capelson, Maya

“…Nuclear pore complexes have emerged in recent years as chromatin-binding nuclear scaffolds, able to influence target gene expression. However, how nucleoporins…”
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Nup98-dependent transcriptional memory is established independently of transcription by Pascual-Garcia, Pau, Little, Shawn C, Capelson, Maya

“…Cellular ability to mount an enhanced transcriptional response upon repeated exposure to external cues is termed transcriptional memory, which can be…”
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Maternal Origins of Developmental Reproducibility by Petkova, Mariela D., Little, Shawn C., Liu, Feng, Gregor, Thomas

“…Cell fate decisions during multicellular development are precisely coordinated, leading to highly reproducible macroscopic structural outcomes [1–3]. The…”
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Correct dosage of X chromosome transcription is controlled by a nuclear pore component by Aleman, Jennifer R, Kuhn, Terra M, Pascual-Garcia, Pau, Gospocic, Janko, Lan, Yemin, Bonasio, Roberto, Little, Shawn C, Capelson, Maya

“…Dosage compensation in Drosophila melanogaster involves a 2-fold transcriptional upregulation of the male X chromosome, which relies on the…”
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Sorting Sloppy Sonic by Little, Shawn C., Gregor, Thomas

“…In the classic picture of morphogen-mediated patterning, cells acquire the correct spatial arrangement of specified fates by reading a precisely distributed…”
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Only accessible information is useful: insights from gradient-mediated patterning by Tikhonov, Mikhail, Little, Shawn C., Gregor, Thomas

“…Information theory is gaining popularity as a tool to characterize performance of biological systems. However, information is commonly quantified without…”
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Shifting Patterns: Merging Molecules, Morphogens, Motility, and Methodology by Little, Shawn C., Wieschaus, Eric F.

“…We highlight crucial technological progress of the past ten years that permits quantitative analysis of cellular behavior. Adapting these methods to the study…”
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Twisted gastrulation promotes BMP signaling in zebrafish dorsal-ventral axial patterning by Little, Shawn C, Mullins, Mary C

“…In vertebrates and invertebrates, the bone morphogenetic protein (BMP) signaling pathway patterns cell fates along the dorsoventral (DV) axis. In vertebrates,…”
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BMP heterodimers signal via distinct type I receptor class functions by Tajer, Benjamin, Dutko, James A., Little, Shawn C., Mullins, Mary C.

“…Heterodimeric TGF-β ligands outperform homodimers in a variety of developmental, cell culture, and therapeutic contexts; however, the mechanisms underlying…”
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Bone morphogenetic protein heterodimers assemble heteromeric type I receptor complexes to pattern the dorsoventral axis by Little, Shawn C, Mullins, Mary C

“…Patterning the embryonic dorsoventral axis of both vertebrates and invertebrates requires signalling through bone morphogenetic proteins (BMPs). Although a…”
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Single mRNA Molecule Detection in Drosophila by Little, Shawn C, Gregor, Thomas

“…Single molecule fluorescent in situ hybridization (smFISH) enables quantitative measurements of gene expression and mRNA localization. The technique is…”
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Independent and coordinate trafficking of single Drosophila germ plasm mRNAs by Little, Shawn C., Sinsimer, Kristina S., Lee, Jack J., Wieschaus, Eric F., Gavis, Elizabeth R.

“…Messenger RNA localization is a conserved mechanism for spatial control of protein synthesis, with key roles in generating cellular and developmental…”
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Using Single Molecule RNA FISH to Determine Nuclear Export and Transcription Phenotypes in Drosophila Tissues by Aleman, Jennifer R, Little, Shawn C, Capelson, Maya

“…Single molecule RNA fluorescence in situ hybridization (smRNA FISH) is a widely used method for examining cellular localization of RNA and assessing gene…”
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The embryo as a laboratory: quantifying transcription in Drosophila by Gregor, Thomas, Garcia, Hernan G, Little, Shawn C

“…Highlights • Early Drosophila embryos possess many features advantageous to studying transcription. • New methods to measure gene expression developed in…”
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Oncogenic Notch Promotes Long-Range Regulatory Interactions within Hyperconnected 3D Cliques by Petrovic, Jelena, Zhou, Yeqiao, Fasolino, Maria, Goldman, Naomi, Schwartz, Gregory W., Mumbach, Maxwell R., Nguyen, Son C., Rome, Kelly S., Sela, Yogev, Zapataro, Zachary, Blacklow, Stephen C., Kruhlak, Michael J., Shi, Junwei, Aster, Jon C., Joyce, Eric F., Little, Shawn C., Vahedi, Golnaz, Pear, Warren S., Faryabi, Robert B.

“…Chromatin loops enable transcription-factor-bound distal enhancers to interact with their target promoters to regulate transcriptional programs. Although…”
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Genetic Variation in Type 1 Diabetes Reconfigures the 3D Chromatin Organization of T Cells and Alters Gene Expression by Fasolino, Maria, Goldman, Naomi, Wang, Wenliang, Cattau, Benjamin, Zhou, Yeqiao, Petrovic, Jelena, Link, Verena M., Cote, Allison, Chandra, Aditi, Silverman, Michael, Joyce, Eric F., Little, Shawn C., Kaestner, Klaus H., Naji, Ali, Raj, Arjun, Henao-Mejia, Jorge, Faryabi, Robert B., Vahedi, Golnaz

“…Genetics is a major determinant of susceptibility to autoimmune disorders. Here, we examined whether genome organization provides resilience or susceptibility…”
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