Search Results - "Lippow, Shaun M."

Computational design of antibody-affinity improvement beyond in vivo maturation by Wittrup, K Dane, Tidor, Bruce, Lippow, Shaun M

“…Antibodies are used extensively in diagnostics and as therapeutic agents. Achieving high-affinity binding is important for expanding detection limits,…”
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Progress in computational protein design by Lippow, Shaun M, Tidor, Bruce

“…Current progress in computational structure-based protein design is reviewed in the areas of methodology and applications. Foundational advances include new…”
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Improved mutants from directed evolution are biased to orthologous substitutions by Cochran, Jennifer R., Kim, Yong-Sung, Lippow, Shaun M., Rao, Balaji, Wittrup, K.Dane

“…We have engineered human epidermal growth factor (EGF) by directed evolution through yeast surface display for significantly enhanced affinity for the EGF…”
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Isolating and engineering human antibodies using yeast surface display by Wittrup, K Dane, Chao, Ginger, Lau, Wai L, Hackel, Benjamin J, Sazinsky, Stephen L, Lippow, Shaun M

“…This protocol describes the process of isolating and engineering antibodies or proteins for increased affinity and stability using yeast surface display…”
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A candidate antibody drug for prevention of malaria by Williams, Katherine L., Guerrero, Steve, Flores-Garcia, Yevel, Kim, Dongkyoon, Williamson, Kevin S., Siska, Christine, Smidt, Pauline, Jepson, Sofia Z., Li, Kan, Dennison, S. Moses, Mathis-Torres, Shamika, Chen, Xiaomu, Wille-Reece, Ulrike, MacGill, Randall S., Walker, Michael, Jongert, Erik, King, C. Richter, Ockenhouse, Christian, Glanville, Jacob, Moon, James E., Regules, Jason A., Tan, Yann Chong, Cavet, Guy, Lippow, Shaun M., Robinson, William H., Dutta, Sheetij, Tomaras, Georgia D., Zavala, Fidel, Ketchem, Randal R., Emerling, Daniel E.

“…Over 75% of malaria-attributable deaths occur in children under the age of 5 years. However, the first malaria vaccine recommended by the World Health…”
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Mobilization of innate and adaptive antitumor immune responses by the RNP-targeting antibody ATRC-101 by Scholz, Alexander, DeFalco, Jeff, Leung, Yvonne, Aydin, Iraz T, Czupalla, Cathrin J, Cao, Wei, Santos, Daniel, Vad, Nikhil, Lippow, Shaun M, Baia, Gilson, Harbell, Michael, Sapugay, Judevin, Zhang, Danhui, Wu, Dai-Chen, Wechsler, Erin, Ye, Anne Z, Wu, Jenny W, Peng, Xiao, Vivian, John, Kaplan, Hargita, Collins, Rodney, Nguyen, Ngan, Whidden, Mark, Kim, Dongkyoon, Millward, Carl, Benjamin, Jonathan, Greenberg, Norman M, Serafini, Tito A, Emerling, Daniel E, Steinman, Lawrence, Robinson, William H, Manning-Bog, Amy

“…Immunotherapy approaches focusing on T cells have provided breakthroughs in treating solid tumors. However, there remains an opportunity to drive anticancer…”
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Pairwise selection assembly for sequence-independent construction of long-length DNA by Blake, William J, Chapman, Brad A, Zindal, Anuradha, Lee, Michael E, Lippow, Shaun M, Baynes, Brian M

“…The engineering of biological components has been facilitated by de novo synthesis of gene-length DNA. Biological engineering at the level of pathways and…”
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Evolution of an Interloop Disulfide Bond in High-Affinity Antibody Mimics Based on Fibronectin Type III Domain and Selected by Yeast Surface Display: Molecular Convergence with Single-Domain Camelid and Shark Antibodies by Lipovšek, Daša, Lippow, Shaun M., Hackel, Benjamin J., Gregson, Melissa W., Cheng, Paul, Kapila, Atul, Wittrup, K. Dane

“…The 10th human fibronectin type III domain ( 10Fn3) is one of several protein scaffolds used to design and select families of proteins that bind with high…”
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Creation of a type IIS restriction endonuclease with a long recognition sequence by Lippow, Shaun M, Aha, Patti M, Parker, Matthew H, Blake, William J, Baynes, Brian M, Lipovšek, Daša

“…Type IIS restriction endonucleases cleave DNA outside their recognition sequences, and are therefore particularly useful in the assembly of DNA from smaller…”
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Rotamer optimization for protein design through MAP estimation and problem-size reduction by Hong, Eun-Jong, Lippow, Shaun M, Tidor, Bruce, Lozano-Pérez, Tomás

“…The search for the global minimum energy conformation (GMEC) of protein side chains is an important computational challenge in protein structure prediction and…”
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Numerical integration techniques for curved-element discretizations of molecule-solvent interfaces by Bardhan, Jaydeep P, Altman, Michael D, Willis, David J, Lippow, Shaun M, Tidor, Bruce, White, Jacob K

“…Surface formulations of biophysical modeling problems offer attractive theoretical and computational properties. Numerical simulations based on these…”
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Engineering Enzyme Specificity Using Computational Design of a Defined-Sequence Library by Lippow, Shaun M., Moon, Tae Seok, Basu, Subhayu, Yoon, Sang-Hwal, Li, Xiazhen, Chapman, Brad A., Robison, Keith, Lipovšek, Daša, Prather, Kristala L.J.

“…Engineered biosynthetic pathways have the potential to produce high-value molecules from inexpensive feedstocks, but a key limitation is engineering enzymes…”
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Long-Acting IL-33 Mobilizes High-Quality Hematopoietic Stem and Progenitor Cells More Efficiently Than Granulocyte Colony-Stimulating Factor or AMD3100 by Alt, Carsten, Yuan, Shujun, Wu, Faye, Stankovich, Basha, Calaoagan, Joy, Schopies, Sandra, Wu, Josephine Y., Shen, Jun, Schneider, Doug, Zhu, Ying, Zhao, Xiao-Yan, Lippow, Shaun M., Bauzon, Maxine

“…•IL-33 mobilizes hematopoietic stem and progenitor cells.•Cells include hematopoietic stem, lymphoid, and endothelial progenitor cells.•Long-acting,…”
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Abstract CT203: First-in-human phase 1b study of ATRC-101, a patient-derived antibody with a tumor-specific target, as monotherapy or in combination with pembrolizumab, in patients with solid tumors by Benjamin, Jonathan E., Higgins, Nicholas, Millward, Carl, DeFalco, Jeff, Manning-Bog, Amy, Aydin, Iraz T., Zhang, Danhui, Lippow, Shaun M., Scholz, Alexander, Leung, Yvonne, Zhu, Yanhong, Sedello, Anna, Rogers, Zane, Dhawan, Ish K., Emerling, Daniel, Robinson, William H., Serafini, Tito A., Greenberg, Norman M.

“…Abstract Background: ATRC-101 is a fully human, engineered IgG1 version of an antibody discovered through a target-agnostic process designed to identify…”
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Long-Acting, Oxidation-Resistant IL-33 Mobilizes High-Quality Hematopoietic Stem and Progenitor Cells More Efficiently Than Wild-Type IL-33, G-CSF or AMD3100 by Alt, Carsten, Wu, Faye, Stankovich, Basha, Calaoagan, Joy, Schopies, Sandra, Wu, Josephine, Liu, Perry, Wong, Billy, Kauser, Katalin, Bringmann, Peter, Shen, JUN, Schneider, Doug, Zhu, Ying, Thompson, Thomas, Lippow, Shaun M., Yuan, Shujun, Bauzon, Maxine

“…▪ Mobilization and collection of peripheral blood hematopoietic stem and progenitor cells (HSPC) has become increasingly important for hematopoietic cell…”
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