Search Results - "Lipman, Everett A."

Quantifying internal friction in unfolded and intrinsically disordered proteins with single-molecule spectroscopy by Soranno, Andrea, Buchli, Brigitte, Nettels, Daniel, Cheng, Ryan R, Müller-Späth, Sonja, Pfeil, Shawn H, Hoffmann, Armin, Lipman, Everett A, Makarov, Dmitrii E, Schuler, Benjamin

“…Internal friction, which reflects the “roughness” of the energy landscape, plays an important role for proteins by modulating the dynamics of their folding and…”
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Localizing internal friction along the reaction coordinate of protein folding by combining ensemble and single-molecule fluorescence spectroscopy by Borgia, Alessandro, Wensley, Beth G., Soranno, Andrea, Nettels, Daniel, Borgia, Madeleine B., Hoffmann, Armin, Pfeil, Shawn H., Lipman, Everett A., Clarke, Jane, Schuler, Benjamin

“…Theory, simulations and experimental results have suggested an important role of internal friction in the kinetics of protein folding. Recent experiments on…”
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Random coil negative control reproduces the discrepancy between scattering and FRET measurements of denatured protein dimensions by Watkins, Herschel M., Simon, Anna J., Sosnick, Tobin R., Lipman, Everett A., Hjelm, Rex P., Plaxco, Kevin W.

“…Small-angle scattering studies generally indicate that the dimensions of unfolded single-domain proteins are independent (to within experimental uncertainty of…”
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Polyproline and the "Spectroscopic Ruler" Revisited with Single-Molecule Fluorescence by Schuler, Benjamin, Lipman, Everett A., Steinbach, Peter J., Kumke, Michael, Eaton, William A., Hochstrasser, Robin M.

“…To determine whether Förster resonance energy transfer (FRET) measurements can provide quantitative distance information in single-molecule fluorescence…”
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Single-Molecule Measurement of Protein Folding Kinetics by Lipman, Everett A., Schuler, Benjamin, Bakajin, Olgica, Eaton, William A.

“…In order to investigate the behavior of single molecules under conditions far from equilibrium, we have coupled a microfabricated laminar-flow mixer to a…”
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Single-molecule spectroscopy of protein folding in a chaperonin cage by Hofmann, Hagen, Hillger, Frank, Pfeil, Shawn H., Hoffmann, Armin, Streich, Daniel, Haenni, Dominik, Nettels, Daniel, Lipman, Everett A., Schuler, Benjamin, Horwich, Arthur L.

“…Molecular chaperones are known to be essential for avoiding protein aggregation in vivo, but it is still unclear how they affect protein folding mechanisms. We…”
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Role of Denatured-State Properties in Chaperonin Action Probed by Single-Molecule Spectroscopy by Hofmann, Hagen, Hillger, Frank, Delley, Cyrille, Hoffmann, Armin, Pfeil, Shawn H., Nettels, Daniel, Lipman, Everett A., Schuler, Benjamin

“…The bacterial chaperonin GroEL/GroES assists folding of a broad spectrum of denatured and misfolded proteins. Here, we explore the limits of this remarkable…”
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Tracking DNA Synthesis with Single-Molecule Strand Displacement by Wickersham, Charles E, Lipman, Everett A

“…We have previously shown that double-stranded DNA labeled with a periodic series of fluorescent dyes can be used to track a single helicase. Here we…”
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Probing the free-energy surface for protein folding with single-molecule fluorescence spectroscopy by Eaton, William A, Schuler, Benjamin, Lipman, Everett A

“…Protein folding is inherently a heterogeneous process because of the very large number of microscopic pathways that connect the myriad unfolded conformations…”
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A microfluidic mixing system for single-molecule measurements by Pfeil, Shawn H, Wickersham, Charles E, Hoffmann, Armin, Lipman, Everett A

“…This article describes the design and fabrication of a microfluidic mixing system optimized for ultrasensitive optical measurements. Channels are…”
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A comparison of the folding kinetics of a small, artificially selected DNA aptamer with those of equivalently simple naturally occurring proteins by Lawrence, Camille, Vallée‐Bélisle, Alexis, Pfeil, Shawn H., Mornay, Derek, Lipman, Everett A., Plaxco, Kevin W.

“…The folding of larger proteins generally differs from the folding of similarly large nucleic acids in the number and stability of the intermediates involved…”
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Synthesis of Extended Nanoscale Optical Encoders by Wickersham, Charles E., Kerr, Daniel H. S., Lipman, Everett A.

“…An optical encoder is a device that uses an interrupted light source−sensor pair to map linear or rotational motion onto a periodic signal. Simple, inexpensive…”
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Tracking a Molecular Motor with a Nanoscale Optical Encoder by Wickersham, Charles E, Cash, Kevin J, Pfeil, Shawn H, Bruck, Irina, Kaplan, Daniel L, Plaxco, Kevin W, Lipman, Everett A

“…Optical encoders are commonly used in macroscopic machines to make precise measurements of distance and velocity by translating motion into a periodic signal…”
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Tracking Processive DNA Synthesis with Single-Molecule Fluorescence by Wickersham, Charles E., Lipman, Everett A.

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Maximizing Photon Counts and Time Resolution for Single Molecule Fluorescence Studies in Micro Fluidic Mixing System by Doerr, Denis, Kuenemuth, Ralf, Pfeil, Shawn H., Lipman, Everett A., Seidel, Claus A.M.

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Hierarchical interconnections in the nano-composite material bone: Fibrillar cross-links resist fracture on several length scales by Fantner, Georg E., Rabinovych, Olexandr, Schitter, Georg, Thurner, Philipp, Kindt, Johannes H., Finch, Marquesa M., Weaver, James C., Golde, Laura S., Morse, Daniel E., Lipman, Everett A., Rangelow, Ivo W., Hansma, Paul K.

“…Bone is a complex and very important multi-constituent bio-composite. In this work, we focus on the arrangement of bone constituents from the nanoscopic to the…”
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Synthesis of Extended Single-Molecule Optical Encoders by Wickersham, Charles E., Kerr, Daniel H., Cash, Kevin J., Pfeil, Shawn H., Bruck, Irina, Kaplan, Daniel L., Plaxco, Kevin W., Lipman, Everett A.

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corrigendum: Probing the free-energy surface for protein folding with single-molecule fluorescence spectroscopy by Schuler, Benjamin, Lipman, Everett A, Eaton, William A

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Erratum: corrigendum: Probing the free-energy surface for protein folding with single-molecule fluorescence spectroscopy by Schuler, Benjamin, Lipman, Everett A., Eaton, William A.

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Hierarchical interconnections in the nano-composite material bone : Fibrillar cross-links resist fracture on several length scales: Nanocomposites by FANTNER, Georg E, RABINOVYCH, Olexandr, RANGELOW, Ivo W, HANSMA, Paul K, SCHITTER, Georg, THURNER, Philipp, KINDT, Johannes H, FINCH, Marquesa M, WEAVER, James C, GOLDE, Laura S, MORSE, Daniel E, LIPMAN, Everett A

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