Search Results - "Lin, John Y."

A user's guide to channelrhodopsin variants: features, limitations and future developments by Lin, John Y.

“…Channelrhodopsins (ChRs) are light‐activated channels from algae that provide these organisms with fast sensors to visible light for phototaxis. Since its…”
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ReaChR: a red-shifted variant of channelrhodopsin enables deep transcranial optogenetic excitation by Lin, John Y, Knutsen, Per Magne, Muller, Arnaud, Kleinfeld, David, Tsien, Roger Y

“…In this technical report, the authors describe a new, red-shifted variant of channelrhodopsin (called red-activatable channelrhodopsin or ReaChR) that shows…”
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The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins by Rodriguez, Erik A., Campbell, Robert E., Lin, John Y., Lin, Michael Z., Miyawaki, Atsushi, Palmer, Amy E., Shu, Xiaokun, Zhang, Jin, Tsien, Roger Y.

“…Over the past 20 years, protein engineering has been extensively used to improve and modify the fundamental properties of fluorescent proteins (FPs) with the…”
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Optogenetic Inhibition of Synaptic Release with Chromophore-Assisted Light Inactivation (CALI) by Lin, John Y., Sann, Sharon B., Zhou, Keming, Nabavi, Sadegh, Proulx, Christophe D., Malinow, Roberto, Jin, Yishi, Tsien, Roger Y.

“…Optogenetic techniques provide effective ways of manipulating the functions of selected neurons with light. In the current study, we engineered an optogenetic…”
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Optogenetic control of Drosophila using a red-shifted channelrhodopsin reveals experience-dependent influences on courtship by Inagaki, Hidehiko K, Jung, Yonil, Hoopfer, Eric D, Wong, Allan M, Mishra, Neeli, Lin, John Y, Tsien, Roger Y, Anderson, David J

“…A recently described red-shifted channelrhodopsin permits control of complex behaviors in freely moving adult flies and reveals the functional modulation of…”
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Dual-channel circuit mapping reveals sensorimotor convergence in the primary motor cortex by Hooks, Bryan M, Lin, John Y, Guo, Caiying, Svoboda, Karel

“…Cortical cells integrate synaptic input from multiple sources, but how these different inputs are distributed across individual neurons is largely unknown…”
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Fluorescent proteins for in vivo imaging, where's the biliverdin? by Montecinos-Franjola, Felipe, Lin, John Y, Rodriguez, Erik A

“…Noninvasive fluorescent imaging requires far-red and near-infrared fluorescent proteins for deeper imaging. Near-infrared light penetrates biological tissue…”
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High-potency ligands for DREADD imaging and activation in rodents and monkeys by Bonaventura, Jordi, Eldridge, Mark A. G., Hu, Feng, Gomez, Juan L., Sanchez-Soto, Marta, Abramyan, Ara M., Lam, Sherry, Boehm, Matthew A., Ruiz, Christina, Farrell, Mitchell R., Moreno, Andrea, Galal Faress, Islam Mustafa, Andersen, Niels, Lin, John Y., Moaddel, Ruin, Morris, Patrick J., Shi, Lei, Sibley, David R., Mahler, Stephen V., Nabavi, Sadegh, Pomper, Martin G., Bonci, Antonello, Horti, Andrew G., Richmond, Barry J., Michaelides, Michael

“…Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) are a popular chemogenetic technology for manipulation of neuronal activity in…”
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Red‐shifted channelrhodopsin stimulation restores light responses in blind mice, macaque retina, and human retina by Sengupta, Abhishek, Chaffiol, Antoine, Macé, Emilie, Caplette, Romain, Desrosiers, Mélissa, Lampič, Maruša, Forster, Valérie, Marre, Olivier, Lin, John Y, Sahel, José‐Alain, Picaud, Serge, Dalkara, Deniz, Duebel, Jens

“…Targeting the photosensitive ion channel channelrhodopsin‐2 (ChR2) to the retinal circuitry downstream of photoreceptors holds promise in treating vision loss…”
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Structural and photophysical characterization of the small ultra-red fluorescent protein by Maiti, Atanu, Buffalo, Cosmo Z., Saurabh, Saumya, Montecinos-Franjola, Felipe, Hachey, Justin S., Conlon, William J., Tran, Geraldine N., Hassan, Bakar, Walters, Kylie J., Drobizhev, Mikhail, Moerner, W. E., Ghosh, Partho, Matsuo, Hiroshi, Tsien, Roger Y., Lin, John Y., Rodriguez, Erik A.

“…The small Ultra-Red Fluorescent Protein (smURFP) represents a new class of fluorescent protein with exceptional photostability and brightness derived from…”
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Optically monitoring voltage in neurons by photo-induced electron transfer through molecular wires by Miller, Evan W, Lin, John Y, Frady, E. Paxon, Steinbach, Paul A, Kristan, William B. Jr, Tsien, Roger Y

“…Fluorescence imaging is an attractive method for monitoring neuronal activity. A key challenge for optically monitoring voltage is development of sensors that…”
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STIM1 Is Required for Remodeling of the Endoplasmic Reticulum and Microtubule Cytoskeleton in Steering Growth Cones by Pavez, Macarena, Thompson, Adrian C, Arnott, Hayden J, Mitchell, Camilla B, D'Atri, Ilaria, Don, Emily K, Chilton, John K, Scott, Ethan K, Lin, John Y, Young, Kaylene M, Gasperini, Robert J, Foa, Lisa

“…The spatial and temporal regulation of calcium signaling in neuronal growth cones is essential for axon guidance. In growth cones, the endoplasmic reticulum…”
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Optogenetic restoration of high sensitivity vision with bReaChES, a red-shifted channelrhodopsin by Too, Lay Khoon, Shen, Weiyong, Protti, Dario A., Sawatari, Atomu, A. Black, Dylan, Leamey, Catherine A., Y. Huang, Jin, Lee, So-Ra, E. Mathai, Ashish, Lisowski, Leszek, Y. Lin, John, C. Gillies, Mark, Simunovic, Matthew P.

“…The common final pathway to blindness in many forms of retinal degeneration is the death of the light-sensitive primary retinal neurons. However, the normally…”
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Toward the second generation of optogenetic tools by Knöpfel, Thomas, Lin, Michael Z, Levskaya, Anselm, Tian, Lin, Lin, John Y, Boyden, Edward S

“…This mini-symposium aims to provide an integrated perspective on recent developments in optogenetics. Research in this emerging field combines optical methods…”
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Interhemispheric Connectivity Potentiates the Basolateral Amygdalae and Regulates Social Interaction and Memory by Huang, Tzyy-Nan, Hsu, Tsan-Ting, Lin, Ming-Hui, Chuang, Hsiu-Chun, Hu, Hsiao-Tang, Sun, Cheng-Pu, Tao, Mi-Hua, Lin, John Y., Hsueh, Yi-Ping

“…Impaired interhemispheric connectivity is commonly found in various psychiatric disorders, although how interhemispheric connectivity regulates brain function…”
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Engineering a memory with LTD and LTP by Nabavi, Sadegh, Fox, Rocky, Proulx, Christophe D., Lin, John Y., Tsien, Roger Y., Malinow, Roberto

“…A rodent study using optogenetics to induce long-term potentiation and long-term depression provides a causal link between synaptic plasticity and memory…”
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Gene Electrotransfer via Conductivity‐Clamped Electric Field Focusing Pivots Sensori‐Motor DNA Therapeutics: “A Spoonful of Sugar Helps the Medicine Go Down” by Pinyon, Jeremy L., Jonquieres, Georg, Crawford, Edward N., Abed, Amr Al, Power, John M., Klugmann, Matthias, Browne, Cherylea J., Housley, David M., Wise, Andrew K., Fallon, James B., Shepherd, Robert K., Lin, John Y., McMahon, Catherine, McAlpine, David, Birman, Catherine S., Lai, Waikong, Enke, Ya Lang, Carter, Paul M., Patrick, James F., Gay, Robert D., Marie, Corinne, Scherman, Daniel, Lovell, Nigel H., Housley, Gary D.

“…Viral vectors and lipofection‐based gene therapies have dispersion‐dependent transduction/transfection profiles that thwart precise targeting. The study…”
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An optimized triple modality reporter for quantitative in vivo tumor imaging and therapy evaluation by Levin, Rachel A, Felsen, Csilla N, Yang, Jin, Lin, John Y, Whitney, Michael A, Nguyen, Quyen T, Tsien, Roger Y

“…We present an optimized triple modality reporter construct combining a far-red fluorescent protein (E2-Crimson), enhanced firefly luciferase enzyme (Luc2), and…”
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Characterization of Engineered Channelrhodopsin Variants with Improved Properties and Kinetics by Lin, John Y., Lin, Michael Z., Steinbach, Paul, Tsien, Roger Y.

“…Channelrhodopsin 2 (ChR2), a light-activated nonselective cationic channel from Chlamydomonas reinhardtii, has become a useful tool to excite neurons into…”
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A far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein by Rodriguez, Erik A, Tran, Geraldine N, Gross, Larry A, Crisp, Jessica L, Shu, Xiaokun, Lin, John Y, Tsien, Roger Y

“…A bright and photostable far-red fluorescent protein, smURFP, was developed from a cyanobacterial phycobiliprotein. smURFP uniquely binds a highly…”
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