Vasopressin gene expression in rat choroid plexus

Vasopressin (VP) levels in cerebrospinal fluid (CSF) change in response to physiological stimuli and under various pathological conditions. The sources of CSF VP have yet to be clarified, however. In the present study, we provide evidence indicating that VP is synthesized in the choroid plexus, the...

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Bibliographic Details
Published in:	Advances in experimental medicine and biology Vol. 449; p. 59
Main Authors:	Chodobski, A, Wojcik, B E, Loh, Y P, Dodd, K A, Szmydynger-Chodobska, J, Johanson, C E, Demers, D M, Chun, Z G, Limthong, N P
Format:	Journal Article
Language:	English
Published:	United States 1998
Subjects:	Animals Choroid Plexus - metabolism Epithelial Cells - metabolism Male Protein Biosynthesis Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics Transcription, Genetic Vasopressins - biosynthesis Vasopressins - genetics
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Summary:	Vasopressin (VP) levels in cerebrospinal fluid (CSF) change in response to physiological stimuli and under various pathological conditions. The sources of CSF VP have yet to be clarified, however. In the present study, we provide evidence indicating that VP is synthesized in the choroid plexus, the primary site of CSF formation. All experiments were performed on adult male Sprague-Dawley rats. The presence of VP mRNA in choroid plexus epithelium was demonstrated by in situ hybridization histochemistry using the 35S-labeled riboprobe that was complementary to cDNA fragment of rat VP encoding the C-terminus part of proVP. In situ hybridization findings were confirmed by reverse transcriptase-polymerase chain reaction analysis. Immunohistochemistry for VP-associated neurophysin (VP-NP), a polypeptide component of proVP, revealed subapical accumulation of VP-NP-immunopositive product in choroidal epithelial cells. Immunoprecipitation and immunoblotting of choroidal protein extracts with anti-VP-NP antibody demonstrated the presence of a approximately 10-kD polypeptide that was also detected in hypothalamus. We hypothesize that the choroid plexus-derived VP exerts autocrine and/or paracrine effects on tissues near the CSF system.
ISSN:	0065-2598
DOI:	10.1007/978-1-4615-4871-3_6