Ultra-diluted/dynamized doxorubicin reduces the toxicity caused by doxorubicin during the in vitro culture of pig preantral follicles enclosed in ovarian tissue

The present study investigated the effect of adding allopathic doxorubicin (DOX 0.3 µg/mL), the vehicle of ultradiluted/dynamized doxorubicin (0.2 % ethanol), different dynamizations of ultradiluted/dynamized doxorubicin (DOX 6CH, DOX 12CH and DOX 30CH), both in the absence or presence of chemical s...

Full description

Saved in:

Bibliographic Details
Published in:	Reproductive toxicology (Elmsford, N.Y.) Vol. 129; p. 108683
Main Authors:	Lima de Andrade, Ramyres Diego, Palomino, Gaby Judith Quispe, de Queiroz, Isabor Sales Marinho, Bezerra da Silva, Ana Flávia, Ferreira, Anna Clara Accioly, Alves, Bênner Geraldo, de Morais, Selene Maia, Rodrigues, Ana Paula Ribeiro, de Lima, Laritza Ferreira, de Figueiredo, José Ricardo
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01-10-2024
Subjects:	Animals Antibiotics, Antineoplastic - toxicity Antioxidants - pharmacology Catalase - metabolism Collagen Type I - metabolism Collagen Type III - metabolism Doxorubicin - toxicity Female Homeopathy Lipofuscin - metabolism Ovarian Follicle - drug effects Ovarian follicles Ovary - drug effects Oxidative Stress - drug effects Pig Sulfhydryl Compounds - metabolism Swine Tissue Culture Techniques Toxicity Utradiluted/dynamized doxorubicin Utradiluted/dynamized doxorubicin Toxicity Ovarian follicles Homeopathy Pig
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	The present study investigated the effect of adding allopathic doxorubicin (DOX 0.3 µg/mL), the vehicle of ultradiluted/dynamized doxorubicin (0.2 % ethanol), different dynamizations of ultradiluted/dynamized doxorubicin (DOX 6CH, DOX 12CH and DOX 30CH), both in the absence or presence of chemical stress induced by doxorubicin at 0.3 µg/mL on follicular survival and activation, antioxidant capacity of the medium, Catalase activity (CAT), production of reactive protein thiol, maintenance of type I and III collagen fibers and accumulation of lipofuscin in porcine ovarian tissue cultured in vitro for 48 hours. To do this, part of the ovarian tissue fragments was fixed for the uncultured control and the rest were cultured in: MEM (cultured control), DOX 0.3 µg/mL, Ethanol, DOX 6CH, DOX 12CH, DOX 30CH, DOX (0.3 µg/mL) + DOX 6CH, DOX (0.3 µg/mL) + DOX 12CH, DOX (0.3 µg/mL) + DOX 30CH treatments. The results showed that, in general, ultradiluted/dynamized doxorubicin (DOX 6CH, DOX 12CH and DOX 30CH) mitigated the toxic effect of allopathic doxorubicin (0.3 µg/mL) on the morphology of preantral follicles, the content of type I and III collagen fibers, and the production of lipofuscin in the tissue. However, only DOX (0.3 µg/mL) + DOX 6CH attenuated the oxidative stress induced by DOX (0.3 µg/mL), maintaining adequate CAT activity that was similar to the uncultured control. Additionally, when the three isolated ultradiluted/dynamized doxorubicin were considered, only DOX 12CH increased the reduced thiol levels compared to the uncultured control and MEM. In conclusion, supplementing the culture medium with ultradiluted/dynamized DOX (DOX 6CH, DOX 12CH and DOX 30CH) attenuated the toxicity induced by allopathic doxorubicin during the in vitro culture of pig preantral follicles enclosed in ovarian tissue. •DOX ultradiluted/dynamized attenuated the toxicity induced by allopathic doxorubicin.•After 48 h, DOX ultradiluted/dynamized improved the survival of porcine preantral follicles.•The accumulation of lipofuscin was significantly reduced.•Proportions of type I and III collagen fibers and Catalase activity were maintained adequate.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0890-6238 1873-1708 1873-1708
DOI:	10.1016/j.reprotox.2024.108683