Nanoscale hybrid implant surfaces and Osterix‐mediated osseointegration

Nanoscale hybrid implant surfaces and Osterix‐mediated osseointegration

Endosseous implant surface topography directly affects adherent cell responses following implantation. The aim of this study was to examine the impact of nanoscale topographic modification of titanium implants on Osterix gene expression since this gene has been reported as key factor for bone format...

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Bibliographic Details
Published in:Journal of biomedical materials research. Part A Vol. 110; no. 3; pp. 696 - 707
Main Authors: Morandini Rodrigues, Laís, Lima Zutin, Elis A., Sartori, Elisa M., Rizzante, Fabio A. P., Mendonça, Daniela B. S., Krebsbach, Paul H., Jepsen, Karl J., Cooper, Lyndon F., Vasconcellos, Luana M. R., Mendonça, Gustavo
Format: Journal Article
Language:English
Published: Hoboken, USA John Wiley & Sons, Inc 01-03-2022
Wiley Subscription Services, Inc
Subjects:
Adherent cells
Adhesion
Alkaline phosphatase
Animals
Biomedical materials
Bone growth
Bone implants
Bone matrix
Bone sialoprotein
bone‐implant interface
Cell adhesion
Dental Implants
Dentin
Dmp1 protein
Evaluation
Flow cytometry
Gene expression
Gene regulation
Histology
Homeobox
Mice
nanostructured materials
Osseointegration
Osteocalcin
Osteogenesis
Prostheses and Implants
Scanning electron microscopy
Surface Properties
surface topography
Surgical implants
Titanium
Titanium - pharmacology
Topography
Transplants & implants
osseointegration
bone-implant interface
gene expression
surface topography
nanostructured materials
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Summary:Endosseous implant surface topography directly affects adherent cell responses following implantation. The aim of this study was to examine the impact of nanoscale topographic modification of titanium implants on Osterix gene expression since this gene has been reported as key factor for bone formation. Titanium implants with smooth and nanoscale topographies were implanted in the femurs of Osterix‐Cherry mice for 1–21 days. Implant integration was evaluated using scanning electron microscopy (SEM) to evaluate cell adhesion on implant surfaces, histology, and nanotomography (NanoCT) to observe and quantify the formed bone‐to‐implant interface, flow cytometry to quantify of Osterix expressing cells in adjacent tissues, and real‐time PCR (qPCR) to quantify the osteoinductive and osteogenic gene expression of the implant‐adherent cells. SEM revealed topography‐dependent adhesion of cells at early timepoints. NanoCT demonstrated greater bone formation at nanoscale implants and interfacial osteogenesis was confirmed histologically at 7 and 14 days for both smooth and nanosurface implants. Flow cytometry revealed greater numbers of Osterix positive cells in femurs implanted with nanoscale versus smooth implants. Compared to smooth surface implants, nanoscale surface adherent cells expressed higher levels of Osterix (Osx), Alkaline phosphatase (Alp), Paired related homeobox (Prx1), Dentin matrix protein 1 (Dmp1), Bone sialoprotein (Bsp), and Osteocalcin (Ocn). In conclusion, nanoscale surface implants demonstrated greater bone formation associated with higher levels of Osterix expression over the 21‐day healing period with direct evidence of surface‐associated gene regulation involving a nanoscale‐mediated osteoinductive pathway that utilizes Osterix to direct adherent cell osteoinduction.
Bibliography:Funding information
Academy of Osseointegration; International Association for Dental Research; National Institute of Arthritis and Musculoskeletal and Skin Diseases, Grant/Award Number: P30 AR069620
ISSN:1549-3296
1552-4965
DOI:10.1002/jbm.a.37323