Development of a flow-injection analysis system with fluorescence detection for gatifloxacin determination in organized medium

Development of a flow-injection analysis system with fluorescence detection for gatifloxacin determination in organized medium

This work reports the development and optimization of a flow injection analysis system with fluorescence detection (FIA–FLUO) for gatifloxacin (GFX) determination in organized medium. The analytical system was based on the enhanced fluorescence of gatifloxacin in micellar medium containing sodium do...

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Bibliographic Details
Published in:Luminescence (Chichester, England) Vol. 30; no. 3; pp. 337 - 342
Main Authors: Lima Vaz, Monica F., de Oliveira, João Vitor F., Cassella, Ricardo J., Pacheco, Wagner F.
Format: Journal Article
Language:English
Published: England Blackwell Publishing Ltd 01-05-2015
Wiley Subscription Services, Inc
Subjects:
Chromatography, High Pressure Liquid - methods
Equipment Design
flow injection analysis
Flow Injection Analysis - instrumentation
Flow Injection Analysis - methods
Fluorescence
Fluoroquinolones - analysis
gatifloxacin
Hydrogen-Ion Concentration
Limit of Detection
Luminescence
Mathematical analysis
Micelles
Ophthalmic Solutions - analysis
Optimization
pharmaceutical formulations
Samples
Sensitivity and Specificity
Sodium Dodecyl Sulfate
Spectrometry, Fluorescence - methods
Spectrophotometry, Ultraviolet
Statistical analysis
Statistical methods
flow injection analysis
gatifloxacin
fluorescence
pharmaceutical formulations
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Summary:This work reports the development and optimization of a flow injection analysis system with fluorescence detection (FIA–FLUO) for gatifloxacin (GFX) determination in organized medium. The analytical system was based on the enhanced fluorescence of gatifloxacin in micellar medium containing sodium dodecyl sulfate (SDS) at pH 6.0. The influence of physical (carrier flow rate, sample volume and volume of reaction coil) and chemical (pH, concentration of buffer and concentration of SDS) parameters that could affect the performance of the FIA system was evaluated in order to reach optimum conditions in terms of sensitivity and analytical throughput. Under optimized conditions, the FIA–FLUO system allowed the injection of 40 samples per hour with a limit of quantification of 72 µg/L and a RSD of 3.5% at 0.20 mg/L. Real samples of commercial pharmaceutical formulations containing GFX were analyzed, and no statistical difference was observed between the results obtained using the developed system and those obtained using the reference method based on high‐performance liquid chromatography with UV detection. Copyright © 2014 John Wiley & Sons, Ltd.
Bibliography:ArticleID:BIO2736
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ark:/67375/WNG-Z44JK5QB-G
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1522-7235
1522-7243
DOI:10.1002/bio.2736