Characterization of the biochemical, physiological, and medicinal properties of Streptomyces hygroscopicus ACTMS-9H isolated from the Amazon (Brazil)

Actinomycetes are known to produce numerous secondary bioactive metabolites of pharmaceutical interest. The purpose of this study was to isolate, characterize, and investigate the antibacterial, antifungal, and anticancer activities of metabolites produced by Actinobacteria isolated from the rhizosp...

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Published in:	Applied microbiology and biotechnology Vol. 101; no. 2; pp. 711 - 723
Main Authors:	Lima, Sandrine M. A., Melo, Janaína. G. S., Militão, Gardênia C. G., Lima, Gláucia M. S., do Carmo A. Lima, Maria, Aguiar, Jaciana S., Araújo, Renata M., Braz-Filho, Raimundo, Marchand, Pascal, Araújo, Janete M., Silva, Teresinha G.
Format:	Journal Article
Language:	English
Published:	Berlin/Heidelberg Springer Berlin Heidelberg 2017 Springer Springer Nature B.V Springer Verlag
Subjects:	Actinobacteria Analysis Anti-Infective Agents - chemistry Anti-Infective Agents - isolation & purification Antibiotics Antimicrobial agents Antineoplastic Agents - chemistry Antineoplastic Agents - isolation & purification Applied Microbial and Cell Physiology Bacteria Bacteria - drug effects Biological Products - chemistry Biological Products - isolation & purification Biomass Biomedical and Life Sciences Biotechnology Brazil Cancer Cell Line, Tumor Cell Survival - drug effects Cytotoxicity Drug resistance Fermentation Fungi - drug effects Glycerol Health aspects Humans Inhibitory Concentration 50 Life Sciences Metabolites Microbial Genetics and Genomics Microbial Sensitivity Tests Microbiology Microorganisms Natural products Paullinia - growth & development Paullinia cupana Pharmaceutical industry Physiology Rhizosphere Soil Microbiology Spectrum Analysis Streptomyces Streptomyces - chemistry Streptomyces - isolation & purification Streptomyces - metabolism Streptomyces - physiology Streptomyces hygroscopicus Studies Yeast Brazil Actinobacteria Fermentation Elaiophylin Bioautography Brazil Streptomyces hygroscopicus
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Summary:	Actinomycetes are known to produce numerous secondary bioactive metabolites of pharmaceutical interest. The purpose of this study was to isolate, characterize, and investigate the antibacterial, antifungal, and anticancer activities of metabolites produced by Actinobacteria isolated from the rhizosphere of Paullinia cupana . The Actinobacteria was identified as Streptomyces hygroscopicus ACTMS-9H. Based on a bioguided study, the methanolic biomass extract obtained from submerged cultivation had the most potent antibacterial, antifungal, and cytotoxic activities. This extract was partitioned with n -hexane, ethyl acetate, and 2-butanol. Elaiophylin was isolated from the methanolic biomass extract, and its molecular formula was determined (C 54 H 88 O 18 ) based on 1 H and 13 C NMR, IR and MS analyses. The 2-butanol phase was fractionated into four fractions (EB1, EB2A, EB2B, and EB3M). Chemical prospecting indicated the presence of alkaloids, saponins, and reducing sugars in the methanolic extract and 2-butanol phase. The elaiophylin displayed anticancer activity in HEp-2 and HL-60 cells with an IC 50 of 1 μg/mL. The EB1 fraction was selectively toxic to HL-60 cells with IC 50 of 9 ng/mL. Bioautography showed that the EB1 fraction contained an alkaloid with antibacterial and antifungal activities (MIC values ≤1.9 and <3.9 μg/mL, respectively). In conclusion, the EB1 fraction and elaiophylin of S. hygroscopicus have potent antimicrobial, antifungal, and anticancer activities.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0175-7598 1432-0614
DOI:	10.1007/s00253-016-7886-9