Evaluation of a hypothetical protein for serodiagnosis and as a potential marker for post-treatment serological evaluation of tegumentary leishmaniasis patients

The serodiagnosis for tegumentary leishmaniasis (TL) presents problems related to the sensitivity and/or specificity of the tests. In the present study, an enzyme-linked immunosorbent assay (ELISA) technique was used to evaluate the performance from a Leishmania braziliensis hypothetical protein, Lb...

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Published in:	Parasitology research (1987) Vol. 116; no. 4; pp. 1197 - 1206
Main Authors:	Lima, Mariana Pedrosa, Costa, Lourena Emanuele, Duarte, Mariana Costa, Menezes-Souza, Daniel, Salles, Beatriz Cristina Silveira, de Oliveira Santos, Thaís Teodoro, Ramos, Fernanda Fonseca, Chávez-Fumagalli, Miguel Angel, Kursancew, Amanda Christine Silva, Ambrósio, Roberta Passamani, Roatt, Bruno Mendes, Machado-de-Ávila, Ricardo Andrez, Gonçalves, Denise Utsch, Coelho, Eduardo Antonio Ferraz
Format:	Journal Article
Language:	English
Published:	Berlin/Heidelberg Springer Berlin Heidelberg 01-04-2017 Springer Springer Nature B.V
Subjects:	Adult Analysis Animals Antibodies Antibodies, Protozoan - blood Antibodies, Protozoan - immunology Bioinformatics Biomedical and Life Sciences Biomedicine Care and treatment Chagas disease Chagas Disease - diagnosis Chagas Disease - parasitology Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Enzymes Female Humans Immunology Kinesin Kinesin - immunology Leishmania braziliensis - immunology Leishmania infantum - immunology Leishmaniasis Leishmaniasis, Cutaneous - diagnosis Leishmaniasis, Cutaneous - parasitology Male Medical Microbiology Microbiology Middle Aged Mucosa Original Paper Parasites Parasitic diseases Patients Proteins Protozoan Proteins - immunology Sensitivity and Specificity Serologic Tests - methods Tegumentary leishmaniasis Tropical diseases Viral antibodies Young Adult Brazil Taiwan Hypothetical proteins Diagnosis Tegumentary leishmaniasis Bioinformatics tools Follow-up Leishmania braziliensis
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Summary:	The serodiagnosis for tegumentary leishmaniasis (TL) presents problems related to the sensitivity and/or specificity of the tests. In the present study, an enzyme-linked immunosorbent assay (ELISA) technique was used to evaluate the performance from a Leishmania braziliensis hypothetical protein, LbHyM, in an attempt to compare its serological reactivity with a soluble Leishmania antigenic preparation (SLA) for the serodiagnosis of cutaneous (CL) and mucosal (ML) leishmaniasis. LbHyM was predicted to be a kinesin-like protein by bioinformatics tools. Serum samples were collected from both CL and ML patients, as well as from those with Chagas disease and from healthy subjects living in endemic or non-endemic areas of TL. Also, sera were collected from patients before and after the treatments, seeking to evaluate their serological follow-up in relation to the anti-protein and anti-parasite antibody levels. When an ELISA-rLbHyM assay was performed, it proved to be significantly more sensitive than ELISA- L. braziliensis SLA in detecting both CL and ML patients. Also, when using sera from Chagas disease patients, the ELISA-rLbHyM proved to be more specific than ELISA-SLA. The anti-protein and anti-parasite antibody levels were also evaluated 6 months after the treatments, and treated patients showed significantly lower levels of specific-rLbHyM antibodies, when compared to the anti-parasite antibody levels. In conclusion, the ELISA-rLbHyM assay can be considered a confirmatory serological technique for the serodiagnosis of L. braziliensis infection and can also be used in the serological follow-up of treated patients, aiming to correlate the low anti-protein antibody levels with the improvement of the healthy state of the patients.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0932-0113 1432-1955
DOI:	10.1007/s00436-017-5397-y