Synthesis and characterization of ordered mesoporous silica (SBA-15 and SBA-16) for adsorption of biomolecules

Synthesis and characterization of ordered mesoporous silica (SBA-15 and SBA-16) for adsorption of biomolecules

•SBA-16 is reported for the first time for biomolecule adsorption.•SBA-15 showed high adsorption capacity for bovine serum albumin and lysozyme than SBA-16.•SBA-16 shows large adsorption capacities for cellulase. The present work describes the adsorption of biomolecules (bovine serum albumin (BSA),...

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Bibliographic Details
Published in:Microporous and mesoporous materials Vol. 180; pp. 284 - 292
Main Authors: dos Santos, Sandra Maria Lopes, Nogueira, Karina Alexandre Barros, de Souza Gama, Marlon, Lima, Jeann Diniz Ferreira, da Silva Júnior, Ivanildo José, de Azevedo, Diana Cristina Silva
Format: Journal Article
Language:English
Published: San Diego, CA Elsevier Inc 01-11-2013
Elsevier
Subjects:
Adsorption isotherm
Chemistry
Colloidal state and disperse state
Enzyme
Exact sciences and technology
General and physical chemistry
Porous materials
Protein
Separation
Silica
Surface physical chemistry
Separation
Enzyme
Silica
Protein
Adsorption isotherm
Characterization
Binary compound
Synthesis
Adsorption
Porous material
Mesoporosity
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Summary:•SBA-16 is reported for the first time for biomolecule adsorption.•SBA-15 showed high adsorption capacity for bovine serum albumin and lysozyme than SBA-16.•SBA-16 shows large adsorption capacities for cellulase. The present work describes the adsorption of biomolecules (bovine serum albumin (BSA), lysozyme (LYS) and cellulase (CEL)) on ordered mesoporous silicas with different pore diameters (SBA-15 and SBA-16) from buffered solutions. These adsorbents were synthesized by sol–gel and hydrothermal routes and characterized by X-ray diffraction, N2 adsorption/desorption isotherms and transmission electron microscopy (TEM). The results by X-ray diffraction and TEM show that the synthesized materials have distinct degrees of mesoporous ordering. The influence of pH on the adsorption of BSA, LYS and CEL as well the kinetics and adsorption isotherms were evaluated in stirred tanks. Among the materials studied, hydrothermally synthesized SBA-15 showed the highest maximum adsorption capacity of BSA and LYS (329 and 636mg/g, respectively). CEL was best adsorbed in SBA-15 synthesized by the sol–gel route.
ISSN:1387-1811
1873-3093
DOI:10.1016/j.micromeso.2013.06.043