Chemical characteristics of a heteropolysaccharide from Tylopilus ballouii mushroom and its antioxidant and anti-inflammatory activities

•Polysaccharides from mushroom Tylopilus balluii was analyzed by IR, HPLC and NMR.•The antioxidant activity of this fucogalactomannan was assessment in vitro.•This polysaccharide reduced inflammatory effect on paw edema and have antioxidant effect. Fucogalactomannan (FGM) is a non-sulphated polysacc...

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Bibliographic Details
Published in:	Carbohydrate polymers Vol. 144; pp. 400 - 409
Main Authors:	Lima, Adriane T.M., Santos, Marilia N., de Souza, Leonardo A.R., Pinheiro, Thuane S., Paiva, Almino A.O., Dore, Celina M.P.G., Costa, Mariana S.S.P., Santos, Nednaldo D., Baseia, Yuri G., Araújo, Renata M., Leite, Edda L.
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 25-06-2016
Subjects:	Animals Anti-Inflammatory Agents - chemistry Anti-Inflammatory Agents - isolation & purification Anti-Inflammatory Agents - pharmacology Anti-Inflammatory Agents - therapeutic use Antioxidant activities Antioxidants - chemistry Antioxidants - isolation & purification Antioxidants - pharmacology Antioxidants - therapeutic use Basidiomycota - chemistry Edema - drug therapy Edema - pathology Fucogalactomannan Inflammation Mice Molecular Weight Monosaccharides - analysis Mushroom NMR Polysaccharides Polysaccharides - chemistry Polysaccharides - isolation & purification Polysaccharides - pharmacology Polysaccharides - therapeutic use Tylopilus ballouii Fucogalactomannan Tylopilus ballouii NMR Polysaccharides Mushroom Antioxidant activities Inflammation
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Summary:	•Polysaccharides from mushroom Tylopilus balluii was analyzed by IR, HPLC and NMR.•The antioxidant activity of this fucogalactomannan was assessment in vitro.•This polysaccharide reduced inflammatory effect on paw edema and have antioxidant effect. Fucogalactomannan (FGM) is a non-sulphated polysaccharide isolated from the Tylopilus ballouii mushroom. We investigated the chemical characteristics of this FGM using HPLC, chemical methods, and NMR studies (1H, 13C, 1H/13C-HSQC and DEPT-135 spectroscopies) without chemical fragmentation. This polysaccharide consisted primarily of mannose and galactose with variable amounts of fucose and traces of xylose and with MW of 140kDa. Infrared and NMR spectroscopies showed the possible interaction between these polysaccharides and proteins. The antioxidant activity showed for FGM a high inhibition of superoxide and hydroxyl radicals with an IC50 of 1.25 and 1.6mg/mL, respectively. The results of peroxidation tests showed that FGM had an IC50 of 1.72mg/mL. Furthermore, the anti-inflammatory assay showed that FGM reduced edema by 32.8%, 42.0%, and 56% at doses of 30, 50, and 70mg/kg, respectively. Thus, these results suggested a structure and indicated possible anti-inflammatory and antioxidant activities use of these polysaccharides.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0144-8617 1879-1344
DOI:	10.1016/j.carbpol.2016.02.050