Agar with embedded channels to study root growth

Agar with embedded channels to study root growth

Agar have long been used as a growth media for plants. Here, we made agar media with embedded fluidic channels to study the effect of exposure to nutrient solution on root growth and pull-out force. Black Eye bean ( Vigna Unguiculata ) and Mung bean ( Vigna Radiata ) were used in this study due to t...

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Bibliographic Details
Published in:Scientific reports Vol. 10; no. 1; p. 14231
Main Authors: Aziz, Azlan Abdul, Lim, Kai Boon, Rahman, Ena Kartina Abdul, Nurmawati, Muhammad Hanafiah, Zuruzi, Abu Samah
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 28-08-2020
Nature Publishing Group
Nature Portfolio
Subjects:
631/449/2653
639/166/988
639/301/923
639/301/930
639/638/298
Agar
Agar - pharmacology
Beans
Diffusion Chambers, Culture
Drug Delivery Systems - methods
Food
Germination
Germination - drug effects
Growth media
Humanities and Social Sciences
Hydrogels
Mechanical Phenomena
Mechanical properties
multidisciplinary
Nanomaterials
Nanoparticles
Nutrients
Nutrients - metabolism
Nutrients - pharmacology
Phytotoxicity
Plant growth
Plant Roots - growth & development
Plantlets
Root development
Science
Science (multidisciplinary)
Vigna - growth & development
Vigna radiata
Vigna unguiculata
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Summary:Agar have long been used as a growth media for plants. Here, we made agar media with embedded fluidic channels to study the effect of exposure to nutrient solution on root growth and pull-out force. Black Eye bean ( Vigna Unguiculata ) and Mung bean ( Vigna Radiata ) were used in this study due to their rapid root development. Agar media were fabricated using casting process with removable cores to form channels which were subsequently filled with nutrient solution. Upon germination, beans were transplanted onto the agar media and allowed to grow. Pull-out force was determined at 96, 120 and 144 h after germination by applying a force on the hypocotyl above the gel surface. The effect of nutrients was investigated by comparing corresponding data obtained from control plants which have not been exposed to nutrient solution. Pull-out force of Black Eye bean plantlets grown in agar with nutrient solution in channels was greater than those grown in gel without nutrients and was 110% greater after 144 h of germination. Pull-out force of Mung bean plantlets grown in agar with and without nutrient solution was similar. Tap root lengths of Black Eye bean and Mung Bean plantlets grown in agar with nutrient solution are shorter than those grown without nutrient. 
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-71076-w