Plasmodium chabaudi adami: interferon-γ but not IL-2 is essential for the expression of cell-mediated immunity against blood-stage parasites in mice

Cell-mediated immunity (CMI) may be important in immunity against blood-stage malaria. Accordingly, we examined the role of type 1 cytokines in the resolution of Plasmodium chabaudi adami malaria in mice genetically modified to have type 1 cytokine gene defects. Parasitemia was prolonged in double k...

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Bibliographic Details
Published in:	Experimental parasitology Vol. 105; no. 2; pp. 159 - 166
Main Authors:	Batchelder, Joan M, Burns, James M, Cigel, Francine K, Lieberg, Heather, Manning, Dean D, Pepper, Barbara J, Yañez, Deborah M, van der Heyde, Henri, Weidanz, William P
Format:	Journal Article
Language:	English
Published:	San Diego, CA Elsevier Inc 01-10-2003 Elsevier
Subjects:	Animals Antibodies, Protozoan - biosynthesis Biological and medical sciences Enzyme-Linked Immunosorbent Assay Experimental protozoal diseases and models Female Flow Cytometry Fundamental and applied biological sciences. Psychology Immunity, Cellular Immunoglobulin G - biosynthesis Immunoglobulin M - biosynthesis Infectious diseases Interferon-gamma - genetics Interferon-gamma - immunology Interleukin-2 - genetics Interleukin-2 - immunology Lymphocyte Subsets - cytology Lymphocyte Subsets - immunology Malaria - immunology Male Medical sciences Medically important nuisances and vectors, pests of stored products and materials: population survey and control Mice Mice, Knockout Parasitemia - immunology Parasitic diseases Plasmodium chabaudi Plasmodium chabaudi - immunology Protozoal diseases Spleen - cytology Spleen - immunology Vectors. Intermediate hosts Protozoa Apicomplexa Vertebrata Mammalia Mouse Rodentia Parasite Interferon Immunity Blood Plasmodium chabaudi
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Summary:	Cell-mediated immunity (CMI) may be important in immunity against blood-stage malaria. Accordingly, we examined the role of type 1 cytokines in the resolution of Plasmodium chabaudi adami malaria in mice genetically modified to have type 1 cytokine gene defects. Parasitemia was prolonged in double knockout (IL-2 −/−, IFNγ −/−) mice compared to control mice. Despite deficiencies in γδ T cell and B cell subsets, these mice produced anti-malarial antibodies and eventually cured their infections, possibly by antibody-mediated immunity. However, because acute P. c. adami parasitemia may also be suppressed by CMI, the requirements for IL-2 and IFNγ were evaluated in mice lacking B cells and functional IL-2 or IFNγ genes. Acute malaria in J H −/−, IL-2 −/− mice was prolonged, but eventually cured. In contrast, J H −/−, IFNγ −/− mice developed unremitting parasitemia. These data strongly suggest that IFNγ, but not IL-2, plays an essential role in the expression of CMI against P. c. adami infections. This finding may prove useful in developing malarial vaccines aimed at inducing CMI. Index Descriptors and Abbreviations: Plasmodium chabaudi adami (P. c. adami); malaria; rodent; T H1/2 cytokines; infectious immunity-parasites; protozoan diseases; AMI, antibody-mediated immunity; CMI, cell-mediated immunity; KO, knockout; WT, wild-type; HRP, horse-radish peroxidase; B cells, B220 +/Ig + B cells; IL, interleukin
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0014-4894 1090-2449
DOI:	10.1016/j.exppara.2003.12.003