Approaches to functional genomics: potential of matrix-assisted laser desorption ionization–time of flight mass spectrometry combined with separation methods for the analysis of DNA in biological samples

Approaches to functional genomics: potential of matrix-assisted laser desorption ionization–time of flight mass spectrometry combined with separation methods for the analysis of DNA in biological samples

MALDI–TOF MS has potential as a valuable technique in DNA mapping studies and may well be complementary to other approaches to DNA analysis such as gel electrophoresis and sequencing. This study used 2,6-dihydroxyacetophenone (DHAP) mixed with diammonium hydrogen citrate (DAHC) as the matrix. In add...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical sciences and applications Vol. 745; no. 1; pp. 231 - 241
Main Authors: Lichtenwalter, Kay G., Apffel, Alex, Bai, Jian, Chakel, John A., Dai, Yuqin, Hahnenberger, Karen M., Li, Liang, Hancock, William S.
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 04-08-2000
Subjects:
Chromatography, High Pressure Liquid
DNA
DNA - analysis
DNA - isolation & purification
Genome
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
MALDI–TOF mass spectrometry
DNA
Functional genomics
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Summary:MALDI–TOF MS has potential as a valuable technique in DNA mapping studies and may well be complementary to other approaches to DNA analysis such as gel electrophoresis and sequencing. This study used 2,6-dihydroxyacetophenone (DHAP) mixed with diammonium hydrogen citrate (DAHC) as the matrix. In addition, recent technical advances such as time lag focussing (TLF) and better selection of matrices (such as 3-hydroxypicolinic acid (3 HPA) and picolinic acid (PA)) extended the range of DNA fragments that can be studied by this approach. The following samples were investigated: Poly-T mixture (dT 15, 19, 20, 25, 74 and 75), plasmid pBR322 derived oligonucleotides (10, 11, 12, 13, 14, 15, 19, 20 and 50 nucleotides long) and DNA fragments of 25, 36 and 37 base pairs corresponding to a fragment in the restriction map for the gene corresponding to the hexon protein of Adenovirus 2 and 5. The results were contrasted with similar analyses performed by ion-paired reversed-phase HPLC coupled to on-line electrospray mass spectrometry.
ISSN:0378-4347
1387-2273
DOI:10.1016/S0378-4347(00)00131-6