Irradiation of human oral mucosa by 233 nm far UV-C LEDs for the safe inactivation of nosocomial pathogens

Irradiation of human oral mucosa by 233 nm far UV-C LEDs for the safe inactivation of nosocomial pathogens

The inactivation of multi resistant pathogens is an important clinical need. One approach is UV-C irradiation, which was previously not possible in vivo due to cytotoxicity. Recently, far UV-C irradiation at λ < 240 nm was successfully used on skin with negligible damage. A potential application...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports Vol. 13; no. 1; p. 22391
Main Authors: Schleusener, Johannes, Lohan, Silke B., Busch, Loris, Zamudio Díaz, Daniela F., Opitz, Nevin, Sicher, Claudia, Lichtenthäler, Tom, Danker, Kerstin, Dommerich, Steffen, Filler, Thomas, Meinke, Martina C., Zwicker, Paula
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 16-12-2023
Nature Publishing Group
Nature Portfolio
Subjects:
631/45/147
692/420/254
Animals
Antiseptics
Cell viability
Chickens
Chorioallantoic membrane
Cross Infection
Cytotoxicity
Deoxyribonucleic acid
DNA
DNA Damage
Drug resistance
Electrical resistivity
Female
Humanities and Social Sciences
Humans
Inactivation
Irradiation
Irritation
Keratinocytes
Mouth Mucosa
Mucosa
multidisciplinary
Nosocomial infection
Pathogens
Radiation
Science
Science (multidisciplinary)
Skin
Ultraviolet radiation
Ultraviolet Rays
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The inactivation of multi resistant pathogens is an important clinical need. One approach is UV-C irradiation, which was previously not possible in vivo due to cytotoxicity. Recently, far UV-C irradiation at λ < 240 nm was successfully used on skin with negligible damage. A potential application site is the nasal vestibule, where MRSA accumulates and cannot be treated using antiseptics. We irradiated 3D mucosa models and excised human mucosa with 222 and 233 nm far UV-C in comparison to 254 nm and broadband UV-B. Eradication efficiency was evaluated by counting colony forming units; irritation potential was evaluated by hen’s egg-chorioallantoic membrane assay and trans epithelial electrical resistance; cell viability was assessed by MTT. DNA damage and cell protective mechanisms were evaluated immunohistopathologically. On mucosa models, MRSA reduced by ≈ 5 log 10 for 60 mJ/cm 2 irradiation at 233 nm. A slightly increased cell viability was observed after 24 h. Lower doses showed lower irritation potential than the positive controls or commercial mouthwash, while 80 mJ/cm 2 had strong irritation potential. DNA damage occurred only superficially and decreased after 24 h. On excised human mucosa, < 10% of keratinocytes were affected after 150 mJ/cm 2 222 nm or 60 mJ/cm 2 233 nm.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-49745-3