Genotypic and biological characteristics of non-identified strain of spotted fever group rickettsiae isolated in Crimea

Genotypic and biological characteristics of non-identified strain of spotted fever group rickettsiae isolated in Crimea

A strain of rickettsiae, designated Crimea-108, was isolated from ticks Dermacentor marginatus in the Crimea in 1977. Its immunobiological characteristics involve low pathogenicity for experimental animals, moderate infectivity for chick embryos, and antigenic relatedness to spotted fever group (SFG...

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Bibliographic Details
Published in:Acta virologica (Anglickâa verze) Vol. 37; no. 6; p. 475
Main Authors: Balayeva, N M, Demkin, V V, Rydkina, E B, Ignatovich, V F, Artemiev, M I, Lichoded LYa, Genig, V A
Format: Journal Article
Language:English
Published: Slovakia 01-12-1993
Subjects:
Animals
Citrate (si)-Synthase - genetics
Cross Reactions
Dermacentor - microbiology
DNA, Bacterial - genetics
Guinea Pigs
Humans
Immune Sera - immunology
Male
Mice
Polymorphism, Restriction Fragment Length
Rats
Rickettsia - classification
Rickettsia - genetics
Rickettsia - isolation & purification
Rickettsia - pathogenicity
Rickettsia Infections - microbiology
Serotyping
Ukraine
Virulence
Ukraine
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Summary:A strain of rickettsiae, designated Crimea-108, was isolated from ticks Dermacentor marginatus in the Crimea in 1977. Its immunobiological characteristics involve low pathogenicity for experimental animals, moderate infectivity for chick embryos, and antigenic relatedness to spotted fever group (SFG) rickettsiae (R. sibirica, R. conorii, R. akari), especially to R. sibirica. The genotypic characterization of the strain Crimea-108 was carried out in comparison with SFG and typhus group rickettsiae by using restriction fragment length polymorphism (RFLP) analysis and DNA-probe hybridization. The marked similarity was detected between DNA restriction patterns of the strains Crimea-108, R. sibirica and R. conorii, but each of them besides comigrating fragments had specific ones. Genotypic analysis of the strain Crimea-108, the SFG and typhus group rickettsiae by three independent DNA probes, based on R. prowazekii DNA, gave unique hybridization patterns for the Crimea-108 strain with all probes. The obtained data show that the Crimea-108 isolate does not belong to the species of R. sibirica, R. conorii, R. akari. The strain Crimea-108 is a novel strain of SFG rickettsiae for the Crimea region.
ISSN:0001-723X