Globicatella bacteraemia identified by 16S ribosomal RNA gene sequencing

Background:Globicatella are streptococcus-like organisms that have been rarely isolated from clinical specimens. Their epidemiology and clinical significance remain largely unknown. Aims: To describe two cases of Globicatella bacteraemia identified by 16S ribosomal RNA (rRNA) gene sequencing. Method...

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Bibliographic Details
Published in:	Journal of clinical pathology Vol. 59; no. 3; pp. 303 - 307
Main Authors:	Lau, S K P, Woo, P C Y, Li, N K H, Teng, J L L, Leung, K-W, Ng, K H L, Que, T-L, Yuen, K-Y
Format:	Journal Article
Language:	English
Published:	London BMJ Publishing Group Ltd and Association of Clinical Pathologists 01-03-2006 BMJ BMJ Publishing Group LTD BMJ Group
Subjects:	16S rRNA gene sequencing Aged, 80 and over Automation bacteraemia Bacteremia - microbiology Bacteria Bacterial diseases Bacterial sepsis Bacterial Typing Techniques Base Sequence Biological and medical sciences Cross Infection - microbiology Deoxyribonucleic acid DNA Female Genes Genes, rRNA Globicatella GPI gram positive identification Human bacterial diseases Humans Infections Infectious diseases Investigative techniques, diagnostic techniques (general aspects) Laboratories Medical sciences Methods Molecular Sequence Data Original Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Patients Phylogenetics ribosomal RNA RNA, Bacterial - genetics RNA, Ribosomal, 16S - analysis rRNA Sequence Analysis, RNA Sequence Homology Staphylococcal Infections - microbiology Staphylococcus - classification Staphylococcus - genetics Urinary tract diseases Urine Urogenital system Infection Anatomic pathology Nucleotide sequence Ribosomal RNA Ribosomal DNA Bacteriosis Bacteremia
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Summary:	Background:Globicatella are streptococcus-like organisms that have been rarely isolated from clinical specimens. Their epidemiology and clinical significance remain largely unknown. Aims: To describe two cases of Globicatella bacteraemia identified by 16S ribosomal RNA (rRNA) gene sequencing. Methods: Two unidentified streptococcus-like bacteria isolated from blood cultures of patients were subject to 16S rRNA gene sequencing. Results: Two cases of Globicatella bacteraemia were identified by 16S rRNA gene sequencing. In the first case, a gram positive coccus was isolated from the blood culture of an 80 year old woman with diabetes mellitus and nosocomial sepsis, who died the day after developing the bacteraemia. The bacterium was unidentified by conventional phenotypic tests, the Vitek (gram positive identification) and the ATB expression (ID32 Strep) systems. In the second case, a similar bacterium was isolated from the blood culture of a 92 year old woman with polymicrobial acute pyelonephritis complicated by septic shock, who subsequently recovered after antibiotic treatment. 16S rRNA gene sequencing of the two isolates showed 0.5% nucleotide difference from that of G. sulfidifaciens and 0.7% nucleotide difference from that of G. sanguinis, indicating that they were Globicatella species. Conclusions: Because Globicatella is rarely encountered in clinical microbiology laboratories, it may have been overlooked or misidentified in these cases. 16S rRNA gene sequencing is a useful tool to better characterise the epidemiology and clinical significance of Globicatella.
Bibliography:	local:0590303 Correspondence to:  Dr K-Y Yuen  Department of Microbiology, University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong; hkumicro@hkucc.hku.hk ark:/67375/NVC-PJQB04DV-5 PMID:16505283 istex:B287E3E36EEC4BEB22EBD0BF887EF579240700C6 href:jclinpath-59-303.pdf ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Case Study-2 ObjectType-Feature-4 ObjectType-Report-1 ObjectType-Article-3
ISSN:	0021-9746 1472-4146
DOI:	10.1136/jcp.2005.028878