Exercise-induced changes to the fiber type-specific redox state in human skeletal muscle are associated with aerobic capacity

Exercise-induced changes to the fiber type-specific redox state in human skeletal muscle are associated with aerobic capacity

The benefits of exercise involve skeletal muscle redox state alterations of nicotinamide adenine dinucleotide (NAD) and flavin adenine dinucleotide (FAD). We determined the fiber-specific effects of acute exercise on the skeletal muscle redox state in healthy adults. Muscle biopsies were obtained fr...

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Bibliographic Details
Published in:Journal of applied physiology (1985) Vol. 135; no. 3; pp. 508 - 518
Main Authors: Shadiow, James, Miranda, Edwin R, Perkins, Ryan K, Mazo, Corey E, Lin, Zhen, Lewis, Kendell N, Mey, Jacob T, Solomon, Thomas P J, Haus, Jacob M
Format: Journal Article
Language:English
Published: United States American Physiological Society 01-09-2023
Subjects:
Adenine
Adult
Aerobic capacity
Biopsy
Excitation
Exercise
Exercise - physiology
Fibers
Flavin
Flavin-adenine dinucleotide
Flavoproteins
Humans
Metabolism
Muscle Fibers, Skeletal - metabolism
Muscle, Skeletal - physiology
Muscles
Musculoskeletal system
Myosin
Myosin Heavy Chains - metabolism
NAD
NAD - metabolism
NADH
Nicotinamide
Nicotinamide adenine dinucleotide
Oxidation
Oxidation-Reduction
Oxygen consumption
Redox properties
Skeletal muscle
NAD/NADH
oxygen delivery
autofluorescence
exercise physiology
flavoproteins
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Summary:The benefits of exercise involve skeletal muscle redox state alterations of nicotinamide adenine dinucleotide (NAD) and flavin adenine dinucleotide (FAD). We determined the fiber-specific effects of acute exercise on the skeletal muscle redox state in healthy adults. Muscle biopsies were obtained from 19 participants (11 M, 8 F; 26 ± 4 yr) at baseline (fasted) and 30 min and 3 h after treadmill exercise at 80% maximal oxygen consumption (V̇o ). Muscle samples were probed for autofluorescence of NADH (excitation at 340-360 nm) and oxidized flavoproteins (Fp; excitation at 440-470 nm) and subsequently, fiber typed to quantify the redox signatures of individual muscle fibers. Redox state was calculated as the oxidation-to-reduction redox ratio: Fp/(Fp + NADH). At baseline, pair-wise comparisons revealed that the redox ratio of myosin heavy chain (MHC) I fibers was 7.2% higher than MHC IIa ( = 0.023, 95% CI: 5.2, 9.2%) and the redox ratio of MHC IIa was 8.0% higher than MHC IIx ( = 0.035, 95% CI: 6.8, 9.2%). MHC I fibers also displayed greater NADH intensity than MHC IIx ( = 0.007) and greater Fp intensity than both MHC IIa ( = 0.019) and MHC IIx ( < 0.0001). Fp intensities increased in all fiber types (main effect, = 0.039) but redox ratios did not change (main effect, = 0.483) 30 min after exercise. The change in redox ratio was positively correlated with capillary density in MHC I (rho = 0.762, = 0.037), MHC IIa fibers (rho = 0.881, = 0.007), and modestly in MHC IIx fibers (rho = 0. 771, = 0.103). These findings support the use of redox autofluorescence to interrogate skeletal muscle metabolism. This study is the first to use autofluorescent imaging to describe differential redox states within human skeletal muscle fiber types with exercise. Our findings highlight an easy and efficacious technique for assessing skeletal muscle redox in humans.
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ISSN:8750-7587
1522-1601
1522-1601
DOI:10.1152/japplphysiol.00662.2022