Search Results - "Lew, V L"

Cytoplasmic calcium buffers in intact human red cells by Tiffert, T, Lew, V L

“…1. Precise knowledge of the cytoplasmic Ca2+ buffering behaviour in intact human red cells is essential for the characterization of their [Ca2+]i-dependent…”
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Deoxygenation permeabilizes sickle cell anaemia red cells to magnesium and reverses its gradient in the dense cells by Ortiz, O E, Lew, V L, Bookchin, R M

“…1. Our findings of a low total magnesium content in the dense fraction (over 1.118 g ml-1) of sickle cell anaemia (SS) red cells seemed inconsistent with the…”
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Effects of deoxygenation on active and passive Ca2+ transport and cytoplasmic Ca2+ buffering in normal human red cells by Tiffert, T, Etzion, Z, Bookchin, R M, Lew, V L

“…1. The effects of deoxygenation on cytoplasmic Ca2+ buffering, saturated Ca2+ extrusion rate through the Ca2+ pump (Vmax), passive Ca2+ influx and…”
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Effects of deoxygenation on active and passive Ca2+ transport and on the cytoplasmic Ca2+ levels of sickle cell anemia red cells by ETZION, Z, TIFFERT, T, BOOKCHIN, R. M, LEW, V. L

“…Elevated [Ca2+]i in deoxygenated sickle cell anemia (SS) red cells (RBCs) could trigger a major dehydration pathway via the Ca(2+)-sensitive K+ channel. But…”
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Effect of intracellular magnesium on calcium extrusion by the plasma membrane calcium pump of intact human red cells by Raftos, J E, Lew, V L

“…1. The effect of varying the concentration of intracellular magnesium on the Ca(2+)-saturated Ca(2+)-extrusion rate through the Ca2+ pump (phi max) was…”
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Evidence for a direct reticulocyte origin of dense red cells in sickle cell anemia by BOOKCHIN, R. M, ORTIZ, O. E, LEW, V. L

“…To explore our hypothesis of a direct reticulocyte origin of irreversibly sickled cells (ISCs), we fractionated light, reticulocyte-rich, and discocyte-rich…”
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Measurement of the distribution of anion exchange function in normal human red cells by Raftos, J E, Bookchin, R M, Lew, V L

“…1. The aim of the present work was to investigate cell-to-cell variation in anion exchange turnover in normal human red cells. Red cells permeabilized to…”
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Maximal calcium extrusion capacity and stoichiometry of the human red cell calcium pump by Dagher, G, Lew, V L

“…1. The uphill calcium efflux through calcium-saturated pumps in intact red cells was investigated with the aid of a new method, in initial conditions of…”
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Pathophysiology of sickle cell anemia by Bookchin, R M, Lew, V L

“…The anemia results from the markedly shortened circulatory survival of SS cells, together with a limited erythropoietic response. Both independent properties…”
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Apparent Ca2+ dissociation constant of Ca2+ chelators incorporated non-disruptively into intact human red cells by Tiffert, Teresa, Lew, Virgilio L.

“…1. A recently developed method of measuring cytoplasmic Ca2+ buffering in intact red cells was applied to re-evaluate the intracellular Ca2+ binding properties…”
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Detection and separation of human red cells with different calcium contents following uniform calcium permeabilization by GARCIA-SANCHO, J, LEW, V. L

“…1. The human red cell, permeabilized to calcium with the ionophore A23187, is extensively used to study Ca2+ transport and the effects of intracellular Ca2+ on…”
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Mechanism of Spontaneous Inside-out Vesiculation of Red Cell Membranes by Lew, Virgilio L., Hockaday, Austin, Freeman, Carol J., Bookchin, Robert M.

“…In certain conditions, human red cell membranes spontaneously form inside out vesicles within 20 min after hypotonic lysis. Study of the geometry of this…”
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A mathematical model of the volume, pH, and ion content regulation in reticulocytes : application to the pathophysiology of sickle cell dehydration by LEW, V. L, FREEMAN, C. J, ORTIZ, O. E, BOOKCHIN, R. M

“…We developed a mathematical model of the reticulocyte, seeking to explain how a cell with similar volume but much higher ionic traffic than the mature red cell…”
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K(86Rb) transport heterogeneity in the low-density fraction of sickle cell anemia red blood cells by Etzion, Z, Lew, V L, Bookchin, R M

“…Previous studies have suggested ion transport heterogeneity among sickle cell anemia (SS) reticulocytes that could influence their dehydration susceptibility…”
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Inhibition of the calcium pump by high cytosolic Ca2+ in intact human red blood cells by Pereira, A C, Samellas, D, Tiffert, T, Lew, V L

“…1. The inhibitory effect of high intracellular calcium on the saturated Ca2+ efflux through the Ca2+ pump (Vmax) was investigated in intact human red cells…”
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Distribution of chloride permeabilities in normal human red cells by Raftos, J E, Bookchin, R M, Lew, V L

“…1. The rate of dehydration of K+ permeabilized red cells is influenced by their Cl- permeability (PCl). In instances of pathological K+ permeabilization,…”
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Calcium-induced conversion of adenine nucleotides to inosine monophosphate in human red cells by Almaraz, L, García‐Sancho, J, Lew, V L

“…1. When inosine-fed human red cells are permeabilized to calcium by exposure to the ionophore A23187, progressively larger proportions of the cell population…”
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Heterogeneous calcium and adenosine triphosphate distribution in calcium-permeabilized human red cells by GARCIA-SANCHO, J, LEW, V. L

“…1. Calcium permeabilization of inosine-fed human red cells using the divalent cation ionophore A23187 induces pump-leak steady states in which the mean total…”
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Magnitude of calcium influx required to induce dehydration of normal human red cells by Tiffert, T, Spivak, J L, Lew, V L

“…Activation by [Ca2+]i of Ca2+-sensitive K+ channels has long been known to cause dehydration of red cells suspended in low-K, plasma-like media. However, the…”
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Magnesium buffering in intact human red blood cells measured using the ionophore A23187 by Flatman, P W, Lew, V L

“…1. A method was developed for measuring the cytoplasmic magnesium buffering of intact red cells using the divalent cation selective ionophore A23187. Addition…”
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