The Flow Cytometry Study of Cellular Immunity in Rhesus Monkeys after Experimental Infection with SARS CoV 2 Virus

The Flow Cytometry Study of Cellular Immunity in Rhesus Monkeys after Experimental Infection with SARS CoV 2 Virus

Cellular immunity plays an important role in the pathogenesis and formation of protective immune defense against the SARS‑CoV‑2 virus. The aim of the work was to study the cellular immunity of rhesus monkeys applying flow cytometry after experimental infection with the SARS‑CoV‑2 virus. Materials an...

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Bibliographic Details
Published in:Problemy osobo opasnyh infekcij no. 3; pp. 53 - 60
Main Authors: Borisevich, G. V., Kirillova, S. L., Shatokhina, I. V., Lebedev, V. N., Solov’ev, S. S., Syromyatnikova, S. I., Shagarova, N. V., Boyarskaya, N. V., Levkovich, N. G., Solyanik, D. A., Andrus, A. F., Rubtsov, V. V., Krotkov, V. T., Kulish, V. S., Surovyatkina, I. V., Kirillov, V. B., Koval’chuk, A. V., Pantyukhov, V. B., Kutaev, D. A., Borisevich, S. V.
Format: Journal Article
Language:English
Russian
Published: Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” 29-10-2022
Subjects:
flow cytometry
lymphocytes
monocytes
rhesus monkeys
sars cov 2-virus
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Summary:Cellular immunity plays an important role in the pathogenesis and formation of protective immune defense against the SARS‑CoV‑2 virus. The aim of the work was to study the cellular immunity of rhesus monkeys applying flow cytometry after experimental infection with the SARS‑CoV‑2 virus. Materials and methods . Male rhesus monkeys were intranasally inoculated with the SARS‑CoV‑2 virus, Isolate B strain and hCoV-19/Russia/SP48-1226/2020 strain (abbreviated name U-2), at a dose of 5.0 lg PFU. Using flow cytometry, the levels of 21 populations/subpopulations of mononuclear cells in the peripheral blood of animals were determined before experimental infection with the pathogen and on day 14 after infection. SARS‑CoV‑2 coronavirus RNA was assessed using real-time polymerase chain reaction. Determination of the titer of virus-neutralizing antibodies to the SARS‑CoV‑2 virus in the blood sera of animals was conducted through neutralization test evaluating the ability to suppress negative colonies. Results and discussion . Infection with Isolate B strain culture has led to an increase in the relative content of total T-lymphocytes (p˂0.2), cytotoxic T-lymphocytes (p˂0.1), as well as monocytes expressing the early activation marker CD25 (p˂0.2). The decrease in levels has been observed for total B-lymphocytes (p˂0.2) and T-helper cells (p˂0.1). Infection with the U-2 strain culture revealed an increase in the relative content of monocytes expressing the early activation marker CD25 (p˂0.2). Thus, for the first time in the Russian Federation, flow cytometry was used to study the cellular immunity of rhesus monkeys before and after experimental infection with the SARS‑CoV‑2 virus. The obtained information can be used for studying the pathogenesis of SARS‑CoV‑2 infection, course, and outcome of the disease, and developing strategies for vaccination and treatment.
ISSN:0370-1069
2658-719X
DOI:10.21055/0370-1069-2022-3-53-60