Search Results - "Levene, Michael J"

Chronic Cellular Imaging of Entire Cortical Columns in Awake Mice Using Microprisms by Andermann, Mark L., Gilfoy, Nathan B., Goldey, Glenn J., Sachdev, Robert N.S., Wölfel, Markus, McCormick, David A., Reid, R. Clay, Levene, Michael J.

“…Two-photon imaging of cortical neurons in vivo has provided unique insights into the structure, function, and plasticity of cortical networks, but this method…”
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Microprisms for In Vivo Multilayer Cortical Imaging by Chia, Thomas H, Levene, Michael J

“…Department of Biomedical Engineering, Yale University, New Haven, Connecticut Submitted 12 November 2008; accepted in final form 29 May 2009 Cortical slices…”
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High-resolution, 2- and 3-dimensional imaging of uncut, unembedded tissue biopsy samples by Torres, Richard, Vesuna, Sam, Levene, Michael J

“…Despite continuing advances in tissue processing automation, traditional embedding, cutting, and staining methods limit our ability for rapid, comprehensive…”
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Biaxial Stretch Improves Elastic Fiber Maturation, Collagen Arrangement, and Mechanical Properties in Engineered Arteries by Huang, Angela H, Balestrini, Jenna L, Udelsman, Brooks V, Zhou, Kevin C, Zhao, Liping, Ferruzzi, Jacopo, Starcher, Barry C, Levene, Michael J, Humphrey, Jay D, Niklason, Laura E

“…Tissue-engineered blood vessels (TEVs) are typically produced using the pulsatile, uniaxial circumferential stretch to mechanically condition and strengthen…”
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In Vivo Multiphoton Microscopy of Deep Brain Tissue by Levene, Michael J, Dombeck, Daniel A, Kasischke, Karl A, Molloy, Raymond P, Webb, Watt W

“…School of Applied and Engineering Physics, Cornell University, Ithaca, New York 14853 Submitted 20 October 2003; accepted in final form 1 December 2003…”
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Multiphoton microscopy of cleared mouse organs by Parra, Sonia, Chia, Thomas H, Zinter, Joseph P, Levene, Michael J

“…Typical imaging depths with multiphoton microscopy (MPM) are limited to less than 300 m in many tissues due to light scattering. Optical clearing significantly…”
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Multiphoton microscopy with clearing for three dimensional histology of kidney biopsies by Olson, Eben, Levene, Michael J, Torres, Richard

“…We present a multiphoton microscopy approach with clearing optimized for pathology evaluation producing image quality comparable to traditional histology. Use…”
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Three-Dimensional Morphology by Multiphoton Microscopy with Clearing in a Model of Cisplatin-Induced CKD by Torres, Richard, Velazquez, Heino, Chang, John J, Levene, Michael J, Moeckel, Gilbert, Desir, Gary V, Safirstein, Robert

“…Traditional histologic methods are limited in their ability to detect pathologic changes of CKD, of which cisplatin therapy is an important cause. In addition,…”
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Maximizing fluorescence collection efficiency in multiphoton microscopy by Zinter, Joseph P, Levene, Michael J

“…Understanding fluorescence propagation through a multiphoton microscope is of critical importance in designing high performance systems capable of deep tissue…”
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Initial Evaluation of Rapid, Direct-to-Digital Prostate Biopsy Pathology by Torres, Richard, Olson, Eben, Homer, Robert, Martin, Darryl T, Levene, Michael J, Perincheri, Sudhir, Sprenkle, Preston C, Humphrey, Peter A

“…Pathologist interobserver discordance is significant in grading of prostate cancer, limiting reliability. Diagnostic reproducibility may be improved with…”
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Singlet gradient index lens for deep in vivo multiphoton microscopy by Murray, Teresa A, Levene, Michael J

“…Micro-optical probes, including gradient index (GRIN) lenses and microprisms, have expanded the range of in vivo multiphoton microscopy to reach previously…”
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Multiphoton fluorescence lifetime imaging of intrinsic fluorescence in human and rat brain tissue reveals spatially distinct NADH binding by Chia, Thomas H, Williamson, Anne, Spencer, Dennis D, Levene, Michael J

“…Two-photon fluorescence lifetime imaging (FLIM) of molecules can reveal important information on the local microenvironment. NADH, an intrinsic fluorescent…”
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In vivo two-photon microscopy of the hippocampus using glass plugs by Velasco, Mary Grace M, Levene, Michael J

“…Two-photon microscopy has been used in conjunction with micro-optics, such as GRIN lenses, to access subcortical structures in the intact mouse brain. In this…”
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Single-molecule fluorescence imaging of processive myosin with enhanced background suppression using linear zero-mode waveguides (ZMWs) and convex lens induced confinement (CLIC) by Elting, Mary Williard, Leslie, Sabrina R, Churchman, L Stirling, Korlach, Jonas, McFaul, Christopher M J, Leith, Jason S, Levene, Michael J, Cohen, Adam E, Spudich, James A

“…Resolving single fluorescent molecules in the presence of high fluorophore concentrations remains a challenge in single-molecule biophysics that limits our…”
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Clinical Measurement of von Willebrand Factor by Fluorescence Correlation Spectroscopy by TORRES, Richard, GENZEN, Jonathan R, LEVENE, Michael J

“…Identification of von Willebrand factor (vWF) abnormalities in a variety of conditions is hampered by the limitations of currently available diagnostic tests…”
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Multiphoton fluorescence, second harmonic generation, and fluorescence lifetime imaging of whole cleared mouse organs by Vesuna, Sam, Torres, Richard, Levene, Michael J

“…Multiphoton microscopy of cleared tissue has previously been demonstrated to generate large three-dimensional (3D) volumetric image data on entire intact mouse…”
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Detection of counterfeit U.S. paper money using intrinsic fluorescence lifetime by Chia, Thomas H, Levene, Michael J

“…Genuine U.S. Federal Reserve Notes have a consistent, two-component intrinsic fluorescence lifetime. This allows for detection of counterfeit paper money…”
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Integrated fluorescence correlation spectroscopy device for point-of-care clinical applications by Olson, Eben, Torres, Richard, Levene, Michael J

“…We describe an optical system which reduces the cost and complexity of fluorescence correlation spectroscopy (FCS), intended to increase the suitability of the…”
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The use of optical clearing and multiphoton microscopy for investigation of three-dimensional tissue-engineered constructs by Calle, Elizabeth A, Vesuna, Sam, Dimitrievska, Sashka, Zhou, Kevin, Huang, Angela, Zhao, Liping, Niklason, Laura E, Levene, Michael J

“…Recent advances in three-dimensional (3D) tissue engineering have concomitantly generated a need for new methods to visualize and assess the tissue. In…”
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Multiphoton microscopy of cleared mouse brain expressing YFP by Parra, Sonia G, Vesuna, Sam S, Murray, Teresa A, Levene, Michael J

“…Multiphoton microscopy of intrinsic fluorescence and second harmonic generation (SHG) of whole mouse organs is made possible by optically clearing the organ…”
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