Large accumulations of maize streak virus in the filter chamber and midgut cells of the leafhopper vector Cicadulina mbila

Maize streak virus (MSV, Mastrevirus, Geminiviridae) is persistently transmitted by Cicadulina mbila, apparently without propagation in its leafhopper vector. MSV was shown earlier by quantitative PCR to accumulate in the alimentary canal of C. mbila. We examined the alimentary canals of C. mbila le...

Full description

Saved in:

Bibliographic Details
Published in:	Archives of virology Vol. 154; no. 2; pp. 255 - 262
Main Authors:	Ammar, El-Desouky, Gargani, Daniel, Lett, Jean M, Peterschmitt, Michel
Format:	Journal Article
Language:	English
Published:	Vienna Vienna : Springer Vienna 01-02-2009 Springer Vienna Springer Springer Nature B.V
Subjects:	Animals Biological and medical sciences Biomedical and Life Sciences Biomedicine Cicadulina mbila Electrons Endosomes - virology Epithelial Cells - virology Fundamental and applied biological sciences. Psychology Gastrointestinal Tract - virology Geminiviridae Hemiptera - virology Immunohistochemistry Infectious Diseases Insect Vectors - virology Maize streak virus Maize streak virus - physiology Mastrevirus Medical Microbiology Microbiology Microscopy, Electron, Transmission Miscellaneous Original Article Phytopathology. Animal pests. Plant and forest protection Plant Diseases - virology Plant viruses and viroids Transmission electron microscopy Virology Virus Internalization Viruses Zea mays - virology France Malpighian Tubule Alimentary Canal Maize Streak Virus Tomato Yellow Leaf Curl Virus Midgut Epithelial Cell Virus Geminiviridae Plant pathogen Maize streak virus Vector Mastrevirus
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Maize streak virus (MSV, Mastrevirus, Geminiviridae) is persistently transmitted by Cicadulina mbila, apparently without propagation in its leafhopper vector. MSV was shown earlier by quantitative PCR to accumulate in the alimentary canal of C. mbila. We examined the alimentary canals of C. mbila leafhoppers that acquired MSV from diseased plants for various acquisition access periods (AAP) by immunofluorescence confocal laser scanning microscopy (iCLSM) and by immunogold labelling transmission electron microscopy (iTEM). Following a 7-day AAP and a 7-day inoculation period (IP) on healthy seedlings, MSV was detected by iCLSM mainly in the filter chamber and anterior midgut. Using iTEM, large accumulations of MSV particles, usually enclosed in membranous vesicles, were detected only in cells of the midgut, inside and outside the filter chamber, following 14- or 30-day AAPs, and also following 7-day AAP and 7-day IP on healthy plants. No virus was detected in the control non-vector species C. chinaï. Coated pits or vesicles, typical of clathrin-mediated endocytosis, were not observed. We discuss an alternative endocytosis pathway and suggest that the MSV accumulations are stored in endosomes in the midgut epithelial cells.
Bibliography:	http://dx.doi.org/10.1007/s00705-008-0308-2 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0304-8608 1432-8798
DOI:	10.1007/s00705-008-0308-2