Biodegradable Polymer Scaffolds for Tissue Engineering

Biodegradable Polymer Scaffolds for Tissue Engineering

Synthetic polymer scaffolds designed for cell transplantation were reproducibly made on a large scale and studied with respect to biocompatibility, structure and biodegradation rate. Polyglycolic acid (PGA) was extruded and oriented to form 13 microns diameter fibers with desired tenacity. Textile p...

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Bibliographic Details
Published in:Bio/technology (New York, N.Y. 1983) Vol. 12; no. 7; pp. 689 - 693
Main Authors: Freed, Lisa E, Vunjak-Novakovic, Gordana, Biron, Robert J, Eagles, Dana B, Lesnoy, Daniel C, Barlow, Sandra K, Langer, Robert
Format: Journal Article
Language:English
Published: New York, NY Nature Publications 01-07-1994
Subjects:
Animal cells
Biocompatible Materials
Biodegradation, Environmental
Biological and medical sciences
Biotechnology
Cartilage - chemistry
Cartilage - cytology
Cartilage - physiology
Cell Transplantation
Cells, Cultured
Collagen - analysis
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Glycosaminoglycans - analysis
Methods. Procedures. Technologies
Miscellaneous
Polyglycolic Acid - chemistry
Polyglycolic Acid - metabolism
Polymers - chemistry
Polymers - metabolism
Prostheses and Implants
Regeneration
Biodegradation
Culture medium
Cartilage
Cell culture
Chondrocyte
Implant
Animal
Polymer
Transplantation
Glycolic acid polymer
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Summary:Synthetic polymer scaffolds designed for cell transplantation were reproducibly made on a large scale and studied with respect to biocompatibility, structure and biodegradation rate. Polyglycolic acid (PGA) was extruded and oriented to form 13 microns diameter fibers with desired tenacity. Textile processing techniques were used to produce fibrous scaffolds with a porosity of 97% and sufficient structural integrity to maintain their dimensions when seeded with isolated cartilage cells (chondrocytes) and cultured in vitro at 37 degrees C for 8 weeks. Cartilaginous tissue consisting of glycosaminoglycan and collagen was regenerated in the shape of the original PGA scaffold. The resulting cell-polymer constructs were the largest grown in vitro to date (1 cm diameter x 0.35 cm thick). Construct mass was accurately predicted by accounting for accumulation of tissue components and scaffold degradation. The scaffold induced chondrocyte differentiation with respect to morphology and phenotype and represents a model cell culture substrate that may be useful for a variety of tissue engineering applications.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
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ISSN:0733-222X
2331-3684
DOI:10.1038/nbt0794-689