Search Results - "Lesnicki, G."

On-line monitoring and control of methanol concentration in shake-flask cultures of Pichia pastoris by Guarna, M.M, Lesnicki, G.J, Tam, B.M, Robinson, J, Radziminski, C.Z, Hasenwinkle, D, Boraston, A, Jervis, E, MacGillivray, R.T.A, Turner, R.F.B, Kolburn, D.G

“…The methylotrophic yeast Pichia pastoris can be used to express recombinant genes at high levels under the control of the methanol-inducible alcohol oxidase 1…”
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Effects of methanol concentration on expression levels of recombinant protein in fed-batch cultures of Pichia methanolica by Mayson, Brian E., Kilburn, Douglas G., Zamost, Bruce L., Raymond, Christopher K., Lesnicki, Gary J.

“…The methylotrophic yeast Pichia methanolica can be used to express recombinant genes at high levels under the control of the methanol‐inducible alcohol oxidase…”
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Expression Analysis of a Modified Factor X in Stably Transformed Insect Cell Lines by Pfeifer, T.A., Guarna, M.M., Kwan, E.M., Lesnicki, G., Theilmann, D.A., Grigliatti, T.A., Kilburn, D.G.

“…A modified Factor X protein was combined with a cellulose-binding domain tag and expressed in insect cell lines. The protein, CBDFX, was expressed and secreted…”
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On-line monitoring and control of methanol concentration in shake-flask cultures ofPichia pastoris by Guarna, M. M., Lesnicki, G. J., Tam, B. M., Robinson, J., Radziminski, C. Z., Hasenwinkle, D., Boraston, A., Jervis, E., MacGillivray, R. T. A., Turner, R. F. B., Kilburn, D. G.

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Production of a self-activating CBM-factor X fusion protein in a stable transformed Sf9 insect cell line using high cell density perfusion culture by GORENFLO, Volker M, PFEIFER, Tom A, LESNICKI, Gary, KWAN, Emily M, GRIGLIATTI, Thomas A, KILBURN, Douglas G, PIRET, James M

“…Factor Xa is a serine protease, whose high selectivity can be used to cleave protein tags from recombinant proteins. A fusion protein comprised of a…”
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Very high-level production and export in Escherichia coli of a cellulose binding domain for use in a generic secretion-affinity fusion system by Hasenwinkle, Diane, Jervis, Eric, Kops, Oliver, Liu, Chang, Lesnicki, Gary, Haynes, Charles A., Kilburn, Douglas G.

“…A novel expression vector pTugA, previously constructed in our laboratory, was modified to provide kanamycin resistance (pTugK) and used to direct the…”
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