Search Results - "Lesich, Kathleen A."

Functional anatomy of the mammalian sperm flagellum by Lindemann, Charles B., Lesich, Kathleen A.

“…The eukaryotic flagellum is the organelle responsible for the propulsion of the male gamete in most animals. Without exception, sperm of all mammalian species…”
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Flagellar and ciliary beating: the proven and the possible by Lindemann, Charles B, Lesich, Kathleen A

“…The working mechanism of the eukaryotic flagellar axoneme remains one of nature's most enduring puzzles. The basic mechanical operation of the axoneme is now a…”
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The many modes of flagellar and ciliary beating: Insights from a physical analysis by Lindemann, Charles B., Lesich, Kathleen A.

“…The mechanism that allows the axoneme of eukaryotic cilia and flagella to produce both helical and planar beating is an enduring puzzle. The nine outer…”
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The geometric clutch at 20: stripping gears or gaining traction? by Lindemann, Charles B, Lesich, Kathleen A

“…It has been 20 years since the geometric clutch (GC) hypothesis was first proposed. The core principle of the GC mechanism is fairly simple. When the axoneme…”
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The physiological role of ADP and Mg2+ in maintaining a stable beat cycle in bull sperm by Lindemann, Charles B., dePinho, Tania G., Lesich, Kathleen A.

“…Sperm flagella derive their motive power from the motor protein dynein. In this study, we show that maintenance of the flagellar beat cycle in…”
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The effects of Ca2+ and ADP on dynein switching during the beat cycle of reactivated bull sperm models by Lesich, Kathleen A., dePinho, Tania G., Dionne, Benjamin J., Lindemann, Charles B.

“…Calcium regulation of flagellar motility is the basis for chemotaxis, phototaxis, and hyperactivation responses in eukaryotic flagellates and spermatozoa. Ca2+…”
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The mechanics of cilia and flagella: What we know and what we need to know by Lindemann, Charles B., Lesich, Kathleen A.

“…In this review, we provide a condensed overview of what is currently known about the mechanical functioning of the flagellar/ciliary axoneme. We also present a…”
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Ultrastructural evidence that motility changes caused by variations in ATP, Mg2+, and ADP correlate to conformational changes in reactivated bull sperm axonemes by Lesich, Kathleen A., de Pinho, Tania G., Dang, Loan, Lindemann, Charles B.

“…We report the results of an ultrastructural study of Triton X‐100‐extracted, Mg‐adenosine triphosphate (ATP)‐reactivated bull sperm. We utilized a rapid…”
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Mechanics of the eukaryotic flagellar axoneme: Evidence for structural distortion during bending by Lesich, Kathleen A., dePinho, Tania G., Pelle, Dominic W., Lindemann, Charles B.

“…The sliding doublet mechanism is the established explanation that allows us to understand the process of ciliary and flagellar bending. In this study, we apply…”
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The Calcium Response of Mouse Sperm Flagella: Role of Calcium Ions in the Regulation of Dynein Activity by LESICH, Kathleen A, KELSCH, Courtney B, PONICHTER, Kristen L, DIONNE, Benjamin J, DANG, Loan, LINDEMANN, Charles B

“…Triton X-100-extracted mouse sperm treated with 0.1 mM ATP and 1.0 mM Ca(2+) exhibit an extremely coiled configuration that has been previously described as a…”
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Insights into the Mechanism of ADP Action on Flagellar Motility Derived from Studies on Bull Sperm by Lesich, Kathleen A., Pelle, Dominic W., Lindemann, Charles B.

“…Adenosine diphosphate (ADP) is known to have interesting effects on flagellar motility. Permeabilized and reactivated bull sperm exhibit a marked reduction in…”
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The Counterbend Phenomenon in Dynein-Disabled Rat Sperm Flagella and What It Reveals about the Interdoublet Elasticity by Lindemann, Charles B., Macauley, Lisa J., Lesich, Kathleen A.

“…Rat sperm that have been rendered passive by disabling the dynein motors with 50 μM sodium metavanadate and 0.1 mM ATP exhibit an interesting response to…”
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Functional Deficiencies and a Reduced Response to Calcium in the Flagellum of Mouse Sperm Lacking SPAG16L by Lesich, Kathleen A, Zhang, Zhibing, Kelsch, Courtney B, Ponichter, Kristen L, Strauss, Jerome F. III, Lindemann, Charles B

“…The Spag16L gene codes for a protein that is localized to the central apparatus which is essential for normal sperm motility and male fertility. Sperm from…”
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The Calcium Response of Mouse Sperm Flagella: Role of Calcium Ions in the Regulation of Dynein Activity1 by Lesich, Kathleen A, Kelsch, Courtney B, Ponichter, Kristen L, Dionne, Benjamin J, Dang, Loan, Lindemann, Charles B

“…Triton X-100-extracted mouse sperm treated with 0.1 mM ATP and 1.0 mM Ca2+ exhibit an extremely coiled configuration that has been previously described as a…”
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Detergent-extracted models for the study of cilia or flagella by Lindemann, Charles B, Lesich, Kathleen A

“…Methods for using non-ionic detergents to produce demembranated and reactivated cilia and flagella are described in detail. Demembranated and reactivated cell…”
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Non-Processive Force Generation by Mammalian Axonemal Dynein In Situ on Doublet Microtubules by Lorch, David P., Lesich, Kathleen A., Lindemann, Charles B., Hunt, Alan J.

“…We utilize optical tweezers to examine displacements produced by small numbers of dynein motors located in situ on doublet microtubules from disintegrated…”
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Mechanical properties of the passive sea urchin sperm flagellum by Pelle, Dominic W., Brokaw, Charles J., Lesich, Kathleen A., Lindemann, Charles B.

“…In this study we used Triton X‐100 extracted sea urchin spermatozoa to investigate the mechanical behavior of the basic 9+2 axoneme. The dynein motors were…”
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Functional Deficiencies and a Reduced Response to Calcium in the Flagellum of Mouse Sperm Lacking SPAG16L1 by Lesich, Kathleen A, Zhang, Zhibing, Kelsch, Courtney B, Ponichter, Kristen L, Strauss, Jerome F, Lindemann, Charles B

“…The Spag16L gene codes for a protein that is localized to the central apparatus which is essential for normal sperm motility and male fertility. Sperm from…”
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Direct measurement of the passive stiffness of rat sperm and implications to the mechanism of the calcium response by Schmitz-Lesich, Kathleen A., Lindemann, Charles B.

“…Glass microprobes were used to measure the stiffness of the flagella of Triton X‐100‐extracted rat sperm models. The sperm models were treated with 50 μM…”
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The physiological role of ADP and M g 2+ in maintaining a stable beat cycle in bull sperm by Lindemann, Charles B., dePinho, Tania G., Lesich, Kathleen A.

“…Sperm flagella derive their motive power from the motor protein dynein. In this study, we show that maintenance of the flagellar beat cycle in…”
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