Application of Raman Spectroscopy for Studying the Mechanisms of Neutrophil Activation by Carbon Nanotubes

Application of Raman Spectroscopy for Studying the Mechanisms of Neutrophil Activation by Carbon Nanotubes

Raman spectroscopy was used to determine mechanisms for neutrophil activation upon interaction with multi-walled carbon nanotubes (MWCNTs) possessing CO 2 H groups (MWCNTCO 2 H) and in complexes with DNA (MWCNT–CO 2 H/DNA). Short MWCNT–CO 2 H and MWCNT–CO 2 H/DNA (100–300 nm) were found to interact...

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Bibliographic Details
Published in:Journal of applied spectroscopy Vol. 88; no. 1; pp. 77 - 84
Main Authors: Golubewa, L. N., Kulahava, T. A., Leonik, Yu. S., Shuba, M. V., Semenkova, G. N.
Format: Journal Article
Language:English
Published: New York Springer US 01-03-2021
Springer
Springer Nature B.V
Subjects:
Analytical Chemistry
Atomic/Molecular Structure and Spectra
Carbon
Chlorine
Crystal structure
Crystals
Deoxyribonucleic acid
DNA
Free radicals
Multi wall carbon nanotubes
Muscle proteins
Nanotubes
Neutrophils
Oxidases
Physics
Physics and Astronomy
Raman spectroscopy
Spectrum analysis
Structure
phagocytosis
Raman spectroscopy
neutrophils
chemiluminescence
reactive oxygen species
multi-walled carbon nanotubes
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Summary:Raman spectroscopy was used to determine mechanisms for neutrophil activation upon interaction with multi-walled carbon nanotubes (MWCNTs) possessing CO 2 H groups (MWCNTCO 2 H) and in complexes with DNA (MWCNT–CO 2 H/DNA). Short MWCNT–CO 2 H and MWCNT–CO 2 H/DNA (100–300 nm) were found to interact with neutrophils, causing cytoskeleton reorganization, namely, activation of G-actin to give F-actin in cells. Raman spectroscopy showed that these CNTs are not phagocytized by neutrophils. MWCNT–CO 2 H enhances the generation of reactive oxygen and chlorine species by neutrophils as indicated by an increase in the NADPH–oxidase activity in a cell suspension. The interaction of MWCNT–CO 2 H/DNA leads to inhibition of the oxygen-activating ability of phagocytes and formation of neutrophilic extracellular traps (NET) with the inclusion of carbon nanostructures. The free radical products generated by neutrophils disrupt the crystal structure of MWCNTs.
ISSN:0021-9037
1573-8647
DOI:10.1007/s10812-021-01143-1