Search Results - "Lenarcic, Brigita"

An allosteric site enables fine‐tuning of cathepsin K by diverse effectors by Novinec, Marko, Rebernik, Mateja, Lenarčič, Brigita

“…The cysteine peptidase cathepsin K is a potent collagenolytic enzyme and a promising target for the treatment of osteoporosis. Here, we characterize its…”
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Probing the activity modification space of the cysteine peptidase cathepsin K with novel allosteric modifiers by Novinec, Marko, Lenarčič, Brigita, Baici, Antonio

“…Targeting allosteric sites is gaining increasing recognition as a strategy for modulating the activity of enzymes, especially in drug design. Here we…”
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Cysteine Cathepsin Activity Regulation by Glycosaminoglycans by Novinec, Marko, Turk, Boris, Lenarcic, B.

“…Cysteine cathepsins are a group of enzymes normally found in the endolysosomes where they are primarily involved in intracellular protein turnover but also…”
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The Central Region of Testican-2 Forms a Compact Core and Promotes Cell Migration by Krajnc, Anja, Gaber, Aljaž, Lenarčič, Brigita, Pavšič, Miha

“…Testicans are modular proteoglycans of the extracellular matrix of various tissues where they contribute to matrix integrity and exert cellular effects like…”
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Papain-like peptidases: structure, function, and evolution by Novinec Marko, Lenarčič Brigita

“…Papain-like cysteine peptidases are a diverse family of peptidases found in most known organisms. In eukaryotes, they are divided into multiple evolutionary…”
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The catalytic domain of cathepsin C (dipeptidyl-peptidase I) alone is a fully functional endoprotease by Rebernik, Mateja, Lenarčič, Brigita, Novinec, Marko

“…Cathepsin C is a tetrameric lysosomal protease that acts as a dipeptidyl-peptidase due to the presence of the exclusion domain that is unique among papain-like…”
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Conformational plasticity and evolutionary analysis of the myotilin tandem Ig domains by Puž, Vid, Pavšič, Miha, Lenarčič, Brigita, Djinović-Carugo, Kristina

“…Myotilin is a component of the sarcomere where it plays an important role in organisation and maintenance of Z-disk integrity. This involves direct binding to…”
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Expression and purification of active human 17β-Hydroxysteroid dehydrogenase type 1 from Escherichia coli by Bekić, Sofija S, Plavša, Jovana J, Pavšič, Miha, Lenarčič, Brigita, Petri, Edward T, Ćelić, Andjelka S

“…Breast cancer cell growth is often dependent on the presence of steroidal hormones. The 17β-hydroxysteroid dehydrogenase type 1 isoform (17βHSD1) catalyzes…”
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Clusterin is a specific stabilizer and liberator of extracellular cathepsin K by Novinec, Marko, Lenarčič, Brigita, Baici, Antonio

“…► We have identified clusterin as a cathepsin K-binding protein. ► Clusterin stabilizes cathepsin K at physiological plasma pH. ► Clusterin does not directly…”
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The cytosolic tail of the tumor marker protein Trop2 - a structural switch triggered by phosphorylation by Pavšič, Miha, Ilc, Gregor, Vidmar, Tilen, Plavec, Janez, Lenarčič, Brigita

“…Trop2 is a transmembrane signaling glycoprotein upregulated in stem and carcinoma cells. Proliferation-enhancing signaling involves regulated intramembrane…”
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A simple and efficient protocol for the production of recombinant cathepsin V and other cysteine cathepsins in soluble form in Escherichia coli by Novinec, Marko, Pavšič, Miha, Lenarčič, Brigita

“…► We have produced recombinant procathepsin V in soluble form in Escherichia coli. ► The zymogen was activated autocatalytically at 30°C. ► The protocol also…”
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Identification of new peptide amides as selective cathepsin L inhibitors: The first step towards selective irreversible inhibitors? by Torkar, Ana, Lenarčič, Brigita, Lah, Tamara, Dive, Vincent, Devel, Laurent

“…A small library of peptide amides was designed to profile the cathepsin L active site. Within the cathepsin family of cysteine proteases, the first round of…”
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Interaction between Human Cathepsins K, L, and S and Elastins: MECHANISM OF ELASTINOLYSIS AND INHIBITION BY MACROMOLECULAR INHIBITORS by Novinec, Marko, Grass, Robert N, Stark, Wendelin J, Turk, Vito, Baici, Antonio, Lenarčič, Brigita

“…Proteolytic degradation of elastic fibers is associated with a broad spectrum of pathological conditions such as atherosclerosis and pulmonary emphysema. We…”
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Cathepsin K: a unique collagenolytic cysteine peptidase by Novinec, Marko, Lenarčič, Brigita

“…Cathepsin K has emerged as a promising target for the treatment of osteoporosis in recent years. Initially identified as a papain-like cysteine peptidase…”
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Diversity and evolution of the thyroglobulin type-1 domain superfamily by Novinec, Marko, Kordis, Dusan, Turk, Vito, Lenarcic, Brigita

“…Multidomain proteins are gaining increasing consideration for their puzzling, flexible utilization in nature. The presence of the characteristic thyroglobulin…”
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Cytotoxic and peptidase inhibitory activities of selected non-hepatotoxic cyclic peptides from cyanobacteria by Bubik, Anja, Sedmak, Bojan, Novinec, Marko, Lenarcic, Brigita, Lah, Tamara T

“…Toxic cyanobacterial blooms are a rich source of metabolites having a variety of biological activities. Two main groups of cyclic peptides, depsipeptides and…”
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Thyroglobulin Type-1 Domains in Equistatin Inhibit Both Papain-like Cysteine Proteinases and Cathepsin D by Lenarcic, Brigita, Turk, Vito

“…Equistatin from sea anemone is a protein composed of three thyroglobulin-type 1 domains known to inhibit papain-like cysteine proteinases, papain, and…”
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Recombinant human SMOCs produced by in vitro refolding: Calcium-binding properties and interactions with serum proteins by Novinec, Marko, Kovačič, Lidija, Škrlj, Nives, Turk, Vito, Lenarčič, Brigita

“…Secreted modular calcium-binding (SMOC) proteins are little known members of the BM-40 family of matricellular proteins. SMOC-1 is localized in basement…”
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Expression, crystallization and preliminary X-ray characterization of the human epithelial cell-adhesion molecule ectodomain by Pavšič, Miha, Lenarčič, Brigita

“…The epithelial cell‐adhesion molecule (EpCAM; CD326) is a transmembrane glycoprotein involved in epithelial cell–cell adhesion, cell proliferation and…”
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A novel allosteric mechanism in the cysteine peptidase cathepsin K discovered by computational methods by Novinec, Marko, Korenč, Matevž, Caflisch, Amedeo, Ranganathan, Rama, Lenarčič, Brigita, Baici, Antonio

“…Allosteric modifiers have the potential to fine-tune enzyme activity. Therefore, targeting allosteric sites is gaining increasing recognition as a strategy in…”
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