Molecular characteristics of Mycobacterium tuberculosis drug-resistant isolates from HIV- and HIV+ tuberculosis patients in Russia

High burden of drug-resistant (DR) tuberculosis (TB) is a significant threat to national TB control programs all over the world and in the Russian Federation. Different Mycobacterium tuberculosis (MTB) genotypes are hypothesized to have specific characteristics affecting TB control programs. For exa...

Full description

Saved in:

Bibliographic Details
Published in:	BMC microbiology Vol. 22; no. 1; p. 138
Main Authors:	Panova, Anna E, Vinokurov, Anatoliy S, Shemetova, Anastasiya A, Burmistrova, Irina A, Shulgina, Marina V, Samoilova, Anastasiya G, Vasilyeva, Irina A, Vakhrusheva, Diana V, Umpeleva, Tatiana V, Eremeeva, Nataliya I, Lavrenchuk, Leonid S, Golubeva, Lyudmila A, Danilova, Tatiana I, Vasilyeva, Tatiana B, Ugol'kova, Vera A, Sosova, Nataliya V, Lekhlyaider, Marina V, Gorshkova, Irina A, Romanova, Tatiana A
Format:	Journal Article
Language:	English
Published:	England BioMed Central Ltd 19-05-2022 BioMed Central BMC
Subjects:	Aminoglycosides Beijing Clinical isolates Control programs Distribution DNA sequencing Drug resistance Drug-resistance conferring mutations Epidemiology Ethambutol Fluoroquinolones Gene polymorphism Genetic aspects Genomes Genotype Genotype & phenotype Genotypes HIV Human immunodeficiency virus Identification and classification Isoniazid Laboratories Loci Methods Mutation Mutation rates Mycobacterium tuberculosis Natural history Nucleotide sequencing Patients Phylogeny Polymorphism Rifampin Strains (organisms) Tuberculosis Russia Beijing China Union of Soviet Socialist Republics > USSR China Beijing Mycobacterium tuberculosis Drug-resistance conferring mutations Genotypes
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	High burden of drug-resistant (DR) tuberculosis (TB) is a significant threat to national TB control programs all over the world and in the Russian Federation. Different Mycobacterium tuberculosis (MTB) genotypes are hypothesized to have specific characteristics affecting TB control programs. For example, Beijing strains are supposed to have higher mutation rates compared to strains of other genotypes and subsequently higher capability to develop drug-resistance. Clinical MTB isolates from HIV- and HIV+ patients from four regions of Russia were analyzed for genotypes and mutations conferring resistance to Isoniazid, Rifampicin, Ethambutol, aminoglycosides, and fluoroquinolones. Analysis of genotypes and polymorphism of genomic loci according to the HIV status of the patients - sources of MTB isolates were performed. Studied MTB isolates from HIV- TB patients belonged to 15 genotypes and from HIV + TB patients - to 6 genotypes. Beijing clinical isolates dominated in HIV- (64,7%) and HIV+ (74,4%) groups. Other isolates were of LAM (including LAM1 and LAM9), Ural, and 4 minor groups of genotypes (including 5 subclones T). The spectrum of genotypes in the HIV- group was broader than in the HIV+ group. PR of B0/W148 Beijing was significantly lower than of other Beijing genotypes in susceptible and MDR-XDR isolates. Rates of isolates belonging to non-Beijing genotypes were higher than Beijing in susceptible isolates from HIV- patients. Beijing genotype isolates prevailed in clinical isolates of all drug susceptibility profiles both from HIV- and HIV+ patients, although B0/W148 Beijing genotype did not dominate in this study. Genome loci and mutations polymorphisms were more pronounced in clinical isolates from HIV- patients, than from HIV+.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1471-2180 1471-2180
DOI:	10.1186/s12866-022-02553-7