Immunohistochemical identification of Toxoplasma gondii in tissues from Modified Agglutination Test positive sheep

Immunohistochemical identification of Toxoplasma gondii in tissues from Modified Agglutination Test positive sheep

Toxoplasma gondii is a zoonotic agent of great importance in veterinary and public health. The aim of this study was to identify T. gondii by IHC (immunohistochemistry) in different sheep tissues and to determine if an association exists between the results obtained by this method and those obtained...

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Bibliographic Details
Published in:Veterinary parasitology Vol. 191; no. 3-4; pp. 347 - 352
Main Authors: Silva, A.F., Oliveira, F.C.R., Leite, J.S., Mello, M.F.V., Brandão, F.Z., Leite, R.I.J.C.K., Frazão-Teixeira, E., Lilenbaum, W., Fonseca, A.B.M., Ferreira, A.M.R.
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 31-01-2013
Subjects:
agglutination tests
Agglutination Tests - standards
Agglutination Tests - veterinary
Animals
Antibodies, Protozoan - metabolism
bradyzoites
brain
Brain - parasitology
cross reaction
Diagnosis
diaphragm
Diaphragm - parasitology
heart
Heart - parasitology
histopathology
humans
immunohistochemistry
Immunohistochemistry - standards
Immunohistochemistry - veterinary
Infections
liver
Liver - parasitology
Ovine
parasites
public health
Sarcocystis
Sheep
Sheep Diseases - diagnosis
statistical analysis
titration
Toxoplasma
Toxoplasma gondii
Toxoplasmosis
Toxoplasmosis, Animal - diagnosis
Zoonosis
Ovine
Infections
Zoonosis
Diagnosis
Toxoplasmosis
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Summary:Toxoplasma gondii is a zoonotic agent of great importance in veterinary and public health. The aim of this study was to identify T. gondii by IHC (immunohistochemistry) in different sheep tissues and to determine if an association exists between the results obtained by this method and those obtained by the Modified Agglutination Test (MAT). Tissue specimens of twenty-six sheep seroreactive for T. gondii were selected for histopathological evaluation. The presence of T. gondii was investigated in brain, liver and heart samples by IHC and a possible anti-T. gondii antibody cross reactions with other parasites. McNemar's, Chi-square and Fisher's Exact Tests were applied for the statistical analysis of the results. The analysed tissues showed at least one of the following histopathological changes: mild-to-moderate congestion, focal polymorphonuclear inflammatory infiltrate and multifocal or focal mononuclear inflammatory infiltrate. Sarcocystis spp. were identified in the histological sections from both the heart and diaphragm tissues of 88.5% (23/26) of the animals. A total of 46.2% (12/26) of the T. gondii seroreactive sheep was also positive for T. gondii by IHC in at least one organ (brain, liver or heart). The liver IHC-positivity for T. gondii was statistically equivalent to the global individual IHC-positivity, according to McNemar's test. In addition, IHC allowed the detection of T. gondii in infected animals regardless of the titration observed in the MAT. The statistical difference observed between the three organs when comparing the low titration group, suggested that the heart might be the most suitable organ to detect T. gondii infection by IHC. The IHC results in this study revealed that almost half of MAT positive animals could serve as potential sources of infection for humans because bradyzoites were identified in different tissues, regardless of the MAT titration.
Bibliography:http://dx.doi.org/10.1016/j.vetpar.2012.09.022
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0304-4017
1873-2550
DOI:10.1016/j.vetpar.2012.09.022