Environmental Xenoestrogens Super-Activate a Variant Murine ER Beta in Cholangiocytes

High systemic levels of oestrogens are cholestatic and primary biliary cholangitis (PBC)-which is characterized by hepatic ductular inflammation-is thought to be triggered by exposure to xenobiotics such as those around landfill sites. Xenoestrogens may be a component of this chemical trigger. We th...

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Published in:	Toxicological sciences Vol. 156; no. 1; pp. 54 - 71
Main Authors:	Meyer, Stephanie K, Probert, Philip M E, Lakey, Anne K, Leitch, Alastair C, Blake, Lynsay I, Jowsey, Paul A, Cooke, Martin P, Blain, Peter G, Wright, Matthew C
Format:	Journal Article
Language:	English
Published:	United States Oxford University Press 01-03-2017
Subjects:	Alternative Splicing Animals Bile Ducts - cytology Bile Ducts - drug effects Bile Ducts - metabolism Bile Ducts - pathology Cell Line Cell Line, Tumor Cells, Cultured Cholestasis - chemically induced Cholestasis - metabolism Cholestasis - pathology Cholestasis - prevention & control Environmental Estrogens and Receptor Activation In Bile Duct Epithelium Estrogen Receptor alpha - agonists Estrogen Receptor alpha - antagonists & inhibitors Estrogen Receptor alpha - genetics Estrogen Receptor alpha - metabolism Estrogen Receptor Antagonists - pharmacology Estrogen Receptor beta - agonists Estrogen Receptor beta - antagonists & inhibitors Estrogen Receptor beta - genetics Estrogen Receptor beta - metabolism Estrogens - chemistry Estrogens - isolation & purification Estrogens - toxicity Female Genes, Reporter - drug effects Humans Kinetics Male Mice Mice, Nude Recombinant Proteins - chemistry Recombinant Proteins - metabolism Soil Pollutants - antagonists & inhibitors Soil Pollutants - isolation & purification Soil Pollutants - toxicity United Kingdom Waste Disposal Facilities nuclear receptor estrogen cholestasis liver ER biliary
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Summary:	High systemic levels of oestrogens are cholestatic and primary biliary cholangitis (PBC)-which is characterized by hepatic ductular inflammation-is thought to be triggered by exposure to xenobiotics such as those around landfill sites. Xenoestrogens may be a component of this chemical trigger. We therefore hypothesized that xenoestrogens are present at higher levels in the proximity of landfill sites. To test this hypothesis, soil samples were collected, extracts prepared and biological oestrogenic activity examined using cell-based reporter gene assays. Extracts from several sample sites around a landfill site contained a chemical(s) which activated the human ERα in a dose-dependent manner. Extracts from 3 separate control sampling sites were absent of any detectable activity. The mouse ERα and 2 variant mouse ERβ cDNAs were cloned and extracts from sample sites around a landfill site also activated these receptors. One variant murine ERβ was constitutively active when expressed in cholangiocytes, was readily inactivated by ICI182780 and activated in a dose-responsive, ICI182780-inhibitable manner by oestrogen. However, when this receptor was activated by extracts from landfill site soils, ICI182780 failed to antagonize activation. ERβ was readily detectable in murine cholangiocytes and exposing mice acutely to a pooled ER activating soil extracts also gave rise to a mild cholestatic injury. These data indicate that the environment around landfill sites may contain higher levels of xenoestrogens; that these chemicals have "super-activating" characteristics with a variant ERβ and therefore these chemicals could be a component of a xenobiotic insult that triggers PBC.
ISSN:	1096-6080 1096-0929
DOI:	10.1093/toxsci/kfw234