Search Results - "Leimgruber, Richard M"

SD0006: a potent, selective and orally available inhibitor of p38 kinase by Burnette, Barry L, Selness, Shaun, Devraj, Raj, Jungbluth, Gail, Kurumbail, Ravi, Stillwell, Loreen, Anderson, Gary, Mnich, Stephen, Hirsch, Jeffrey, Compton, Robert, De Ciechi, Pamela, Hope, Heidi, Hepperle, Michael, Keith, Robert H, Naing, Win, Shieh, Huey, Portanova, Joseph, Zhang, Yan, Zhang, Jian, Leimgruber, Richard M, Monahan, Joseph

“…SD0006 is a diarylpyrazole that was prepared as an inhibitor of p38 kinase-alpha (p38alpha). In vitro, SD0006 was selective for p38alpha kinase over 50 other…”
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Development of improved cell lysis, solubilization and imaging approaches for proteomic analyses by Leimgruber, Richard M., Malone, James P., Radabaugh, Melissa R., LaPorte, Michelle L., Violand, Bernard N., Monahan, Joseph B.

“…Analysis of complex biochemical processes at the level of the proteome requires methods that quantitatively solubilize cytosolic and membrane bound proteins…”
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Safety assessment of the neomycin phosphotransferase II (NPTII) protein by Fuchs, R.L, Ream, J.E, Hammond, B.G, Naylor, M.W, Leimgruber, R.M, Berberich, S.A

“…Two approaches were used to assess the safety of the NPTII protein for human consumption using purified E. coli produced NPTII protein that was shown to be…”
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Multiple conformations of a human interleukin‐3 variant by Feng, Yiqing, Hood, William F., Forgey, Robert W., Abegg, Ann L., Caparon, Marie H., Thiele, Barrett R., Leimgruber, Richard M., Mcwherter, Charles A.

“…Interleukin‐3 (IL‐3) is a cytokine that stimulates the proliferation and differentiation of hematopoietic cells. The hyperactive hIL‐3 variant SC‐55494 was…”
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Purification and characterization of microbially expressed neomycin phosphotransferase II (NPTII) protein and its equivalence to the plant expressed protein by Fuchs, R.L, Heeren, R.A, Gustafson, M.E, Rogan, G.J, Bartnicki, D.E, Leimgruber, R.M, Finn, R.F, Hershman, A, Berberich, S.A

“…The gene encoding neomycin phosphotransferase II (NPTII) has been used routinely as a selectable marker in the production of genetically engineered crops. To…”
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Safety Assessment of 1-Aminocyclopropane-1-carboxylic Acid Deaminase Protein Expressed in Delayed Ripening Tomatoes by Reed, Andrew J, Kretzmer, Keith A, Naylor, Mark W, Finn, Rory F, Magin, Kimberly M, Hammond, Bruce G, Leimgruber, Richard M, Rogers, Stephen G, Fuchs, Roy L

“…Tomato plants with delayed fruit ripening have been produced by stable insertion of the gene encoding the 1-aminocyclopropane-1-carboxylic acid deaminase…”
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High Level Expression and Refolding of Mouse Interleukin 4 Synthesized in Escherichia coli(∗) by Leimgruber, Richard M., Bishop, Bruce F., Levine, Alan D., Rangwala, Shaukat H., Horn, Nancy A., Peel, Michelle A., Matthews, Brian K., Manning, Julia A., Olins, Peter O.

“…Mouse Interleukin 4 is a 20-kDa glycoprotein, synthesized by activated T lymphocytes and mast cells, which regulates the growth and/or differentiation of a…”
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Purification and Biochemical Comparison of 1-Aminocyclopropane-1-carboxylic Acid Deaminase Proteins Expressed in Delayed Ripening Tomato and Escherichia coli:  Studies for a Food Safety Assessment by Finn, Rory F, Leimgruber, Richard M, Boyle, Denis M, Jennings, Michael G, Kimack, Nancy M, Smith, Christine E, Bishop, Bruce F, Frazier, Ronald B, Magin, Kimberly M, Fuchs, Roy L, Reed, Andrew J

“…Tomato plants with delayed fruit ripening have been developed by introduction of the gene encoding the 1-aminocyclopropane-1-carboxylic acid deaminase (ACCd)…”
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Secretion of active kringle-2-serine protease in Escherichia coli by Obukowicz, Mark G, Gustafson, Mark E, Junger, Kurt D, Leimgruber, Richard M, Wittwer, Arthur J, Wun, Tze Chein, Warren, Thomas G, Bishop, Bruce F, Mathis, Karl J

“…Active human tissue plasminogen activator variant kringle-2-serine protease (K2 + SP domains; referred to as MB1004) was synthesized as a secreted protein in…”
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Practical aspects of fluorescent staining for proteomic applications by Malone, James P., Radabaugh, Melissa R., Leimgruber, Richard M., Gerstenecker, Gary S.

“…SYPRO Orange and SYPRO Ruby staining methods, modified for use with large‐format two dimensional (2‐D) gels, are compared to the manufacturer's recommended…”
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The glycoprotease of Pasteurella haemolytica A1 eliminates binding of myeloid cells to P-selectin but not to E-selectin by Steininger, C N, Eddy, C A, Leimgruber, R M, Mellors, A, Welply, J K

“…HL-60 cells and neutrophils treated with the glycoprotease from Pasteurella haemolytica A1, an enzyme which is specific for O-sialoglycoproteins, were found to…”
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Characterization of glycosylated bovine placental lactogen and the effect of enzymatic deglycosylation on receptor binding and biological activity by Byatt, J C, Welply, J K, Leimgruber, R M, Collier, R J

“…Bovine placental lactogen (bPL) is a glycoprotein hormone that has both somatogenic and lactogenic properties. Purified preparations of the hormone contain…”
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Functional Analysis of Protein N-Myristoylation: Metabolic Labeling Studies Using Three Oxygen-Substituted Analogs of Myristic Acid and Cultured Mammalian Cells Provide Evidence for Protein-Sequence-Specific Incorporation and Analog-Specific Redistribution by Johnson, D. Russell, Cox, Adrienne D., Solski, Patricia A., Devadas, Balekudru, Adams, Steven P., Leimgruber, Richard M., Heuckeroth, Robert O., Buss, Janice E., Gordon, Jeffrey I.

“…Covalent attachment of myristic acid (C14:0) to the NH2-terminal glycine residue of a number of cellular, viral, and oncogene-encoded proteins is essential for…”
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Renaturation and purification of human tissue factor pathway inhibitor expressed in recombinant E. coli by Gustafson, M E, Junger, K D, Wun, T C, Foy, B A, Diaz-Collier, J A, Welsch, D J, Obukowicz, M G, Bishop, B F, Bild, G S, Leimgruber, R M

“…Tissue factor pathway inhibitor is an inhibitor of the extrinsic coagulation pathway. Evaluation of the pharmacological effects of tissue factor pathway…”
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Comparison of recombinant tissue factor pathway inhibitors expressed in human SK hepatoma, mouse C127, baby hamster kidney, and Chinese hamster ovary cells by Wun, T C, Kretzmer, K K, Palmier, M O, Day, K C, Huang, M D, Welsch, D J, Lewis, C, Wolfe, R A, Zobel, J F, Lange, G W

“…Recombinant tissue factor pathway inhibitor (rTFPI) has been expressed in four mammalian expression systems using human SK hepatoma, mouse C127, baby hamster…”
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Large-Scale Production of HIV-1 Protease from Escherichia coli Using Selective Extraction and Membrane Fractionation by Gustafson, M.E., Junger, K.D., Foy, B.A., Baez, J.A., Bishop, B.F., Rangwala, S.H., Michener, M.L., Leimgruber, R.M., Houseman, K.A., Mueller, R.A., Matthews, B.K., Olins, P.O., Grabner, R.W., Hershman, A.

“…Human immunodeficiency virus type 1 (HIV-1) protease was expressed in Escherichia coli as a fusion protein with the N-terminal sequence of IGF-2. The protein…”
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Crystallization of a chymotrypsin inhibitor from Erythrina caffra seeds by Shieh, H S, Leimgruber, N K, Palmier, M O, Wun, T C, Leimgruber, R M, Abdel-Meguid, S S

“…Crystals of a chymotrypsin inhibitor from Erythrina caffra seeds have been grown out of lithium sulfate, by the hanging drop method of vapor diffusion. The…”
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Irreversible inhibition by acetaldehyde of cholecystokinin-induced amylase secretion from isolated rat pancreatic acini by Sankaran, H, Lewin, M B, Wong, A, Deveney, C W, Wendland, M F, Leimgruber, R M, Geokas, M C

“…Acetaldehyde inhibited both amylase secretion induced by maximal concentrations (300 pM) of cholecystokinin octapeptide and the binding of radioiodinated…”
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Tightly-bound ATP and ADP in reconstituted submitochondrial particles by Leimgruber, R M, Senior, A E

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Accessibility of the alpha chains in membrane-bound and solubilized bacterial ATPase to chymotryptic cleavage by Leimgruber, R M, Jensen, C, Abrams, A

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