Search Results - "Lei, S."

A CRISPR–dCas Toolbox for Genetic Engineering and Synthetic Biology by Xu, Xiaoshu, Qi, Lei S.

“…Programmable control of gene expression is essential to understanding gene function, engineering cellular behaviors, and developing therapeutics. Beyond the…”
Get full text

CRISPR Cas9 in Genome Editing and Beyond by Wang, Haifeng, La Russa, Marie, Qi, Lei S

“…The Cas9 protein (CRISPR-associated protein 9), derived from type II CRISPR (clustered regularly interspaced short palindromic repeats) bacterial immune…”
Get full text

Engineered miniature CRISPR-Cas system for mammalian genome regulation and editing by Xu, Xiaoshu, Chemparathy, Augustine, Zeng, Leiping, Kempton, Hannah R., Shang, Stephen, Nakamura, Muneaki, Qi, Lei S.

“…Compact and versatile CRISPR-Cas systems will enable genome engineering applications through high-efficiency delivery in a wide variety of contexts. Here, we…”
Get full text

The New State of the Art: Cas9 for Gene Activation and Repression by La Russa, Marie F., Qi, Lei S.

“…CRISPR-Cas9 technology has rapidly changed the landscape for how biologists and bioengineers study and manipulate the genome. Derived from the bacterial…”
Get full text

Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation by Gilbert, Luke A., Horlbeck, Max A., Adamson, Britt, Villalta, Jacqueline E., Chen, Yuwen, Whitehead, Evan H., Guimaraes, Carla, Panning, Barbara, Ploegh, Hidde L., Bassik, Michael C., Qi, Lei S., Kampmann, Martin, Weissman, Jonathan S.

“…While the catalog of mammalian transcripts and their expression levels in different cell types and disease states is rapidly expanding, our understanding of…”
Get full text

CRISPR technologies for precise epigenome editing by Nakamura, Muneaki, Gao, Yuchen, Dominguez, Antonia A., Qi, Lei S.

“…The epigenome involves a complex set of cellular processes governing genomic activity. Dissecting this complexity necessitates the development of tools capable…”
Get full text

The causal role of gut microbiota in development of osteoarthritis by Yu, X.-H., Yang, Y.-Q., Cao, R.-R., Bo, L., Lei, S.-F.

“…There is considerable evidence for relationship between gut microbiota and osteoarthritis (OA), but no studies have investigated their causal relationship…”
Get full text

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes by Gilbert, Luke A., Larson, Matthew H., Morsut, Leonardo, Liu, Zairan, Brar, Gloria A., Torres, Sandra E., Stern-Ginossar, Noam, Brandman, Onn, Whitehead, Evan H., Doudna, Jennifer A., Lim, Wendell A., Weissman, Jonathan S., Qi, Lei S.

“…The genetic interrogation and reprogramming of cells requires methods for robust and precise targeting of genes for expression or repression. The…”
Get full text

Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System by Chen, Baohui, Gilbert, Luke A., Cimini, Beth A., Schnitzbauer, Joerg, Zhang, Wei, Li, Gene-Wei, Park, Jason, Blackburn, Elizabeth H., Weissman, Jonathan S., Qi, Lei S., Huang, Bo

“…The spatiotemporal organization and dynamics of chromatin play critical roles in regulating genome function. However, visualizing specific, endogenous genomic…”
Get full text

Beyond editing: repurposing CRISPR–Cas9 for precision genome regulation and interrogation by Dominguez, Antonia A., Lim, Wendell A., Qi, Lei S.

“…Key Points Beyond gene editing, the CRISPR–Cas9 technology offers a versatile sequence-specific gene regulation 'toolset', by utilizing the nuclease-deficient…”
Get full text

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression by Qi, Lei S., Larson, Matthew H., Gilbert, Luke A., Doudna, Jennifer A., Weissman, Jonathan S., Arkin, Adam P., Lim, Wendell A.

“…Targeted gene regulation on a genome-wide scale is a powerful strategy for interrogating, perturbing, and engineering cellular systems. Here, we develop a…”
Get full text

CRISPR-Based Chromatin Remodeling of the Endogenous Oct4 or Sox2 Locus Enables Reprogramming to Pluripotency by Liu, Peng, Chen, Meng, Liu, Yanxia, Qi, Lei S., Ding, Sheng

“…Generation of induced pluripotent stem cells typically requires the ectopic expression of transcription factors to reactivate the pluripotency network…”
Get full text

Applications of CRISPR Genome Engineering in Cell Biology by Wang, Fangyuan, Qi, Lei S

“…Recent advances in genome engineering are starting a revolution in biological research and translational applications. The clustered regularly interspaced…”
Get full text

Small Molecules Enhance CRISPR Genome Editing in Pluripotent Stem Cells by Yu, Chen, Liu, Yanxia, Ma, Tianhua, Liu, Kai, Xu, Shaohua, Zhang, Yu, Liu, Honglei, La Russa, Marie, Xie, Min, Ding, Sheng, Qi, Lei S.

“…The bacterial CRISPR-Cas9 system has emerged as an effective tool for sequence-specific gene knockout through non-homologous end joining (NHEJ), but it remains…”
Get full text

A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging by Tanenbaum, Marvin E., Gilbert, Luke A., Qi, Lei S., Weissman, Jonathan S., Vale, Ronald D.

“…Signals in many biological processes can be amplified by recruiting multiple copies of regulatory proteins to a site of action. Harnessing this principle, we…”
Get full text

Development of CRISPR as an Antiviral Strategy to Combat SARS-CoV-2 and Influenza by Abbott, Timothy R., Dhamdhere, Girija, Liu, Yanxia, Lin, Xueqiu, Goudy, Laine, Zeng, Leiping, Chemparathy, Augustine, Chmura, Stephen, Heaton, Nicholas S., Debs, Robert, Pande, Tara, Endy, Drew, La Russa, Marie F., Lewis, David B., Qi, Lei S.

“…The coronavirus disease 2019 (COVID-19) pandemic, caused by the SARS-CoV-2 virus, has highlighted the need for antiviral approaches that can target emerging…”
Get full text

CRISPR-ERA: a comprehensive design tool for CRISPR-mediated gene editing, repression and activation by Liu, Honglei, Wei, Zheng, Dominguez, Antonia, Li, Yanda, Wang, Xiaowo, Qi, Lei S

“…The CRISPR/Cas9 system was recently developed as a powerful and flexible technology for targeted genome engineering, including genome editing (altering the…”
Get full text

Advances in CRISPR therapeutics by Chavez, Michael, Chen, Xinyi, Finn, Paul B., Qi, Lei S.

“…The clustered regularly interspaced short palindromic repeats (CRISPR) renaissance was catalysed by the discovery that RNA-guided prokaryotic CRISPR-associated…”
Get full text

CRISPR-mediated live imaging of genome editing and transcription by Wang, Haifeng, Nakamura, Muneaki, Abbott, Timothy R, Zhao, Dehua, Luo, Kaiwen, Yu, Cordelia, Nguyen, Cindy M, Lo, Albert, Daley, Timothy P, La Russa, Marie, Liu, Yanxia, Qi, Lei S

“…We report a robust, versatile approach called CRISPR live-cell fluorescent in situ hybridization (LiveFISH) using fluorescent oligonucleotides for genome…”
Get full text

The realization of CRISPR gene therapy by Ma, Yitong, Qi, Lei S.

“…The inaugural CRISPR-based drug Casgevy has been approved by several medical agencies, with other CRISPR-based therapies currently in clinical trials. Although…”
Get full text