Search Results - "Lehnart, Stephan E."

Glyoxal as an alternative fixative to formaldehyde in immunostaining and super‐resolution microscopy by Richter, Katharina N, Revelo, Natalia H, Seitz, Katharina J, Helm, Martin S, Sarkar, Deblina, Saleeb, Rebecca S, D'Este, Elisa, Eberle, Jessica, Wagner, Eva, Vogl, Christian, Lazaro, Diana F, Richter, Frank, Coy‐Vergara, Javier, Coceano, Giovanna, Boyden, Edward S, Duncan, Rory R, Hell, Stefan W, Lauterbach, Marcel A, Lehnart, Stephan E, Moser, Tobias, Outeiro, Tiago F, Rehling, Peter, Schwappach, Blanche, Testa, Ilaria, Zapiec, Bolek, Rizzoli, Silvio O

“…Paraformaldehyde (PFA) is the most commonly used fixative for immunostaining of cells, but has been associated with various problems, ranging from loss of…”
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The molecular and functional identities of atrial cardiomyocytes in health and disease by Brandenburg, Sören, Arakel, Eric C., Schwappach, Blanche, Lehnart, Stephan E.

“…Atrial cardiomyocytes are essential for fluid homeostasis, ventricular filling, and survival, yet their cell biology and physiology are incompletely…”
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Modulation of the ryanodine receptor and intracellular calcium by Zalk, Ran, Lehnart, Stephan E, Marks, Andrew R

“…Ryanodine receptors (RyRs)/Ca2+ release channels, on the endoplasmic and sarcoplasmic reticulum of most cell types, are required for intracellular Ca2+ release…”
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Deconstructing sarcomeric structure–function relations in titin-BioID knock-in mice by Rudolph, Franziska, Fink, Claudia, Hüttemeister, Judith, Kirchner, Marieluise, Radke, Michael H., Lopez Carballo, Jacobo, Wagner, Eva, Kohl, Tobias, Lehnart, Stephan E., Mertins, Philipp, Gotthardt, Michael

“…Proximity proteomics has greatly advanced the analysis of native protein complexes and subcellular structures in culture, but has not been amenable to study…”
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3D Super-Resolution Nuclear Q-FISH Imaging Reveals Cell-Cycle-Related Telomere Changes by Pochechueva, Tatiana V, Schwenzer, Niko, Kohl, Tobias, Brandenburg, Sören, Kaltenecker, Gesa, Wollnik, Bernd, Lehnart, Stephan E

“…We present novel workflows for Q-FISH nanoscopy with the potential for prognostic applications and resolving novel chromatin compaction changes…”
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Optogenetic determination of the myocardial requirements for extrasystoles by cell type-specific targeting of ChannelRhodopsin-2 by Zaglia, Tania, Pianca, Nicola, Borile, Giulia, Da Broi, Francesca, Richter, Claudia, Campione, Marina, Lehnart, Stephan E., Luther, Stefan, Corrado, Domenico, Miquerol, Lucile, Mongillo, Marco

“…Extrasystoles lead to several consequences, ranging from uneventful palpitations to lethal ventricular arrhythmias, in the presence of pathologies, such as…”
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SAP97 and Dystrophin Macromolecular Complexes Determine Two Pools of Cardiac Sodium Channels Nav1.5 in Cardiomyocytes by Petitprez, Séverine, Zmoos, Anne-Flore, Ogrodnik, Jakob, Balse, Elise, Raad, Nour, El-Haou, Said, Albesa, Maxime, Bittihn, Philip, Luther, Stefan, Lehnart, Stephan E, Hatem, Stéphane N, Coulombe, Alain, Abriel, Hugues

“…RATIONALE:The cardiac sodium channel Nav1.5 plays a key role in excitability and conduction. The 3 last residues of Nav1.5 (Ser-Ile-Val) constitute a…”
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Resolving titin’s lifecycle and the spatial organization of protein turnover in mouse cardiomyocytes by Rudolph, Franziska, Hüttemeister, Judith, da Silva Lopes, Katharina, Jüttner, René, Yu, Lily, Bergmann, Nora, Friedrich, Dhana, Preibisch, Stephan, Wagner, Eva, Lehnart, Stephan E., Gregorio, Carol C., Gotthardt, Michael

“…Cardiac protein homeostasis, sarcomere assembly, and integration of titin as the sarcomeric backbone are tightly regulated to facilitate adaptation and repair…”
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Superresolution Modeling of Calcium Release in the Heart by Walker, Mark A., Williams, George S.B., Kohl, Tobias, Lehnart, Stephan E., Jafri, M. Saleet, Greenstein, Joseph L., Lederer, W.J., Winslow, Raimond L.

“…Stable calcium-induced calcium release (CICR) is critical for maintaining normal cellular contraction during cardiac excitation-contraction coupling. The…”
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Editorial: Cardiac optogenetics: Using light to observe and excite the heart by Bruegmann, Tobias, Smith, Godfrey L., Lehnart, Stephan E.

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Phosphorylation of the ryanodine receptor mediates the cardiac fight or flight response in mice by Shan, Jian, Kushnir, Alexander, Betzenhauser, Matthew J, Reiken, Steven, Li, Jingdong, Lehnart, Stephan E, Lindegger, Nicolas, Mongillo, Marco, Mohler, Peter J, Marks, Andrew R

“…During the classic "fight-or-flight" stress response, sympathetic nervous system activation leads to catecholamine release, which increases heart rate and…”
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Calpain cleavage of Junctophilin-2 generates a spectrum of calcium-dependent cleavage products and DNA-rich NT1-fragment domains in cardiomyocytes by Weninger, Gunnar, Pochechueva, Tatiana, El Chami, Dana, Luo, Xiaojing, Kohl, Tobias, Brandenburg, Sören, Urlaub, Henning, Guan, Kaomei, Lenz, Christof, Lehnart, Stephan E.

“…Calpains are calcium-activated neutral proteases involved in the regulation of key signaling pathways. Junctophilin-2 (JP2) is a Calpain-specific proteolytic…”
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Differential Cardiac Remodeling in Preload Versus Afterload by TOISCHER, Karl, ROKITA, Adam G, GREBE, Cornelia, PREUSS, Lena, GUPTA, Shamindra N, SCHMIDT, Kathie, LEHNART, Stephan E, KRÜGER, Martina, LINKE, Wolfgang A, BACKS, Johannes, REGITZ-ZAGROSEK, Vera, SCHÄFER, Katrin, UNSÖLD, Bernhard, FIELD, Loren J, MAIER, Lars S, HASENFUSS, Gerd, WUQIANG ZHU, KARARIGAS, Georgios, SOSSALLA, Samuel, REUTER, Sean P, BECKER, Alexander, TEUCHER, Nils, SEIDLER, Tim

“…Hemodynamic load regulates myocardial function and gene expression. We tested the hypothesis that afterload and preload, despite similar average load, result…”
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Endothelial p53 deletion improves angiogenesis and prevents cardiac fibrosis and heart failure induced by pressure overload in mice by Gogiraju, Rajinikanth, Xu, Xingbo, Bochenek, Magdalena L, Steinbrecher, Julia H, Lehnart, Stephan E, Wenzel, Philip, Kessel, Michael, Zeisberg, Elisabeth M, Dobbelstein, Matthias, Schäfer, Katrin

“…Cardiac dysfunction developing in response to chronic pressure overload is associated with apoptotic cell death and myocardial vessel rarefaction. We examined…”
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Remodeling of Ryanodine Receptor Complex Causes "Leaky" Channels: A Molecular Mechanism for Decreased Exercise Capacity by Bellinger, Andrew M., Reiken, Steven, Dura, Miroslav, Murphy, Peter W., Deng, Shi-Xian, Landry, Donald W., Nieman, David, Lehnart, Stephan E., Samaru, Mahendranauth, LaCampagne, Alain, Marks, Andrew R.

“…During exercise, defects in calcium (Ca²⁺) release have been proposed to impair muscle function. Here, we show that during exercise in mice and humans, the…”
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Ca2+/Calmodulin-Dependent Protein Kinase II Phosphorylation Regulates the Cardiac Ryanodine Receptor by Wehrens, Xander H.T, Lehnart, Stephan E, Reiken, Steven R, Marks, Andrew R

“…ABSTRACT—The cardiac ryanodine receptor (RyR2)/calcium release channel on the sarcoplasmic reticulum is required for muscle excitation-contraction coupling…”
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Intracellular calcium release and cardiac disease by Wehrens, Xander H T, Lehnart, Stephan E, Marks, Andrew R

“…Intracellular calcium release channels are present on sarcoplasmic and endoplasmic reticuli (SR, ER) of all cell types. There are two classes of these…”
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Transgenic Mice for Real-Time Visualization of cGMP in Intact Adult Cardiomyocytes by Götz, Konrad R, Sprenger, Julia U, Perera, Ruwan K, Steinbrecher, Julia H, Lehnart, Stephan E, Kuhn, Michaela, Gorelik, Julia, Balligand, Jean-Luc, Nikolaev, Viacheslav O

“…RATIONALE:3′,5′-Cyclic guanosine monophosphate (cGMP) is an important second messenger that regulates cardiac contractility and protects the heart from…”
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Phosphodiesterase 4D Deficiency in the Ryanodine-Receptor Complex Promotes Heart Failure and Arrhythmias by Lehnart, Stephan E., Wehrens, Xander H.T., Reiken, Steven, Warrier, Sunita, Belevych, Andriy E., Harvey, Robert D., Richter, Wito, Jin, S.-L. Catherine, Conti, Marco, Marks, Andrew R.

“…Phosphodiesterases (PDEs) regulate the local concentration of 3′,5′ cyclic adenosine monophosphate (cAMP) within cells. cAMP activates the cAMP-dependent…”
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Multiphoton Imaging of Ca2+ Instability in Acute Myocardial Slices from a RyR2R2474S Murine Model of Catecholaminergic Polymorphic Ventricular Tachycardia by Borile, Giulia, Zaglia, Tania, E. Lehnart, Stephan, Mongillo, Marco

“…Catecholaminergic Polymorphic Ventricular Tachycardia (CPVT) is a familial stress-induced arrhythmia syndrome, mostly caused by mutations in Ryanodine receptor…”
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