Ultrastructural Analysis of DNA Complexes During Transfection and Intracellular Transport

Ultrastructural Analysis of DNA Complexes During Transfection and Intracellular Transport

We present a simple method based on transmission electron microscopy that allows investigation of the early steps of polyplex-mediated transfection without the use of labeled DNA. The ultrastructural analysis showed internalization of 0.2–1-μm aggregates composed of 30–50-nm subunits. In addition, n...

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Bibliographic Details
Published in:The journal of histochemistry and cytochemistry Vol. 51; no. 9; pp. 1237 - 1240
Main Authors: Cartier, Regis, Velinova, Maria, Lehman, Cathleen, Erdmann, Bettina, Reszka, Regina
Format: Journal Article
Language:English
Published: Los Angeles, CA Histochemical Soc 01-09-2003
SAGE Publications
Subjects:
Biological Transport
Gene Transfer Techniques
Genes, Reporter
Humans
Luciferases - genetics
Luciferases - metabolism
Microscopy, Electron
Peptides - chemistry
Peptides - genetics
Peptides - metabolism
Plasmids - chemistry
Plasmids - metabolism
Plasmids - ultrastructure
Transfection
Tumor Cells, Cultured
gene transfer
NLS
transfection mechanism
polyplexes
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Summary:We present a simple method based on transmission electron microscopy that allows investigation of the early steps of polyplex-mediated transfection without the use of labeled DNA. The ultrastructural analysis showed internalization of 0.2–1-μm aggregates composed of 30–50-nm subunits. In addition, new details of the internalization process were revealed, suggesting an unspecific cell entry mechanism of large DNA aggregates.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0022-1554
1551-5044
DOI:10.1177/002215540305100914