Search Results - "Leger, Adrien"

RNA modifications detection by comparative Nanopore direct RNA sequencing by Leger, Adrien, Amaral, Paulo P., Pandolfini, Luca, Capitanchik, Charlotte, Capraro, Federica, Miano, Valentina, Migliori, Valentina, Toolan-Kerr, Patrick, Sideri, Theodora, Enright, Anton J., Tzelepis, Konstantinos, van Werven, Folkert J., Luscombe, Nicholas M., Barbieri, Isaia, Ule, Jernej, Fitzgerald, Tomas, Birney, Ewan, Leonardi, Tommaso, Kouzarides, Tony

“…RNA molecules undergo a vast array of chemical post-transcriptional modifications (PTMs) that can affect their structure and interaction properties. In recent…”
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pycoMeth: a toolbox for differential methylation testing from Nanopore methylation calls by Snajder, Rene, Leger, Adrien, Stegle, Oliver, Bonder, Marc Jan

“…We present pycoMeth, a toolbox to store, manage and analyze DNA methylation calls from long-read sequencing data obtained using the Oxford Nanopore…”
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The Medaka Inbred Kiyosu-Karlsruhe (MIKK) panel by Fitzgerald, Tomas, Brettell, Ian, Leger, Adrien, Wolf, Nadeshda, Kusminski, Natalja, Monahan, Jack, Barton, Carl, Herder, Cathrin, Aadepu, Narendar, Gierten, Jakob, Becker, Clara, Hammouda, Omar T, Hasel, Eva, Lischik, Colin, Lust, Katharina, Sokolova, Natalia, Suzuki, Risa, Tsingos, Erika, Tavhelidse, Tinatini, Thumberger, Thomas, Watson, Philip, Welz, Bettina, Khouja, Nadia, Naruse, Kiyoshi, Birney, Ewan, Wittbrodt, Joachim, Loosli, Felix

“…Unraveling the relationship between genetic variation and phenotypic traits remains a fundamental challenge in biology. Mapping variants underlying complex…”
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Genomic variations and epigenomic landscape of the Medaka Inbred Kiyosu-Karlsruhe (MIKK) panel by Leger, Adrien, Brettell, Ian, Monahan, Jack, Barton, Carl, Wolf, Nadeshda, Kusminski, Natalja, Herder, Cathrin, Aadepu, Narendar, Becker, Clara, Gierten, Jakob, Hammouda, Omar T, Hasel, Eva, Lischik, Colin, Lust, Katharina, Sokolova, Natalia, Suzuki, Risa, Tavhelidse, Tinatini, Thumberger, Thomas, Tsingos, Erika, Watson, Philip, Welz, Bettina, Naruse, Kiyoshi, Loosli, Felix, Wittbrodt, Joachim, Birney, Ewan, Fitzgerald, Tomas

“…The teleost medaka (Oryzias latipes) is a well-established vertebrate model system, with a long history of genetic research, and multiple high-quality…”
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Identification of miRNAs Involved in Reprogramming Acinar Cells into Insulin Producing Cells by Teichenne, Joan, Morró, Meritxell, Casellas, Alba, Jimenez, Veronica, Tellez, Noelia, Leger, Adrien, Bosch, Fatima, Ayuso, Eduard

“…Reprogramming acinar cells into insulin producing cells using adenoviral (Ad)-mediated delivery of Pdx1, Ngn3 and MafA (PNM) is an innovative approach for the…”
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Genetics instability of wtAAV2 genome and AAV promoter activities in the Baculovirus/Sf9 cells system by Savy, Adrien, Kaikkonen, Minna U, Léger, Adrien, Dickx, Yohann, Galibert, Lionel, Merten, Otto-Wilhelm

“…The human Adeno-Associated Virus serotype 2 (wtAAV2) is a common non-pathological virus and its recombinant form (rAAV) is widely used as gene therapy vector…”
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Advanced Characterization of DNA Molecules in rAAV Vector Preparations by Single-stranded Virus Next-generation Sequencing by Lecomte, Emilie, Tournaire, Benoît, Cogné, Benjamin, Dupont, Jean-Baptiste, Lindenbaum, Pierre, Martin-Fontaine, Mélanie, Broucque, Frédéric, Robin, Cécile, Hebben, Matthias, Merten, Otto-Wilhelm, Blouin, Véronique, François, Achille, Redon, Richard, Moullier, Philippe, Léger, Adrien

“…Recent successful clinical trials with recombinant adeno-associated viral vectors (rAAVs) have led to a renewed interest in gene therapy. However, despite…”
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AAV8 locoregional delivery induces long-term expression of an immunogenic transgene in macaques despite persisting local inflammation by Gernoux, Gwladys, Guilbaud, Mickaël, Devaux, Marie, Journou, Malo, Pichard, Virginie, Jaulin, Nicolas, Léger, Adrien, Le Duff, Johanne, Deschamps, Jack-Yves, Le Guiner, Caroline, Moullier, Philippe, Cherel, Yan, Adjali, Oumeya

“…Adeno-associated virus (AAV) vectors are considered efficient vectors for gene transfer, as illustrated by recent successful clinical trials targeting retinal…”
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Accurate expression quantification from nanopore direct RNA sequencing with NanoCount by Gleeson, Josie, Leger, Adrien, Prawer, Yair D J, Lane, Tracy A, Harrison, Paul J, Haerty, Wilfried, Clark, Michael B

“…Abstract Accurately quantifying gene and isoform expression changes is essential to understanding cell functions, differentiation and disease. Sequencing…”
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Chemical and photochemical error rates in light-directed synthesis of complex DNA libraries by Lietard, Jory, Leger, Adrien, Erlich, Yaniv, Sadowski, Norah, Timp, Winston, Somoza, Mark M

“…Abstract Nucleic acid microarrays are the only tools that can supply very large oligonucleotide libraries, cornerstones of the nascent fields of de novo gene…”
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RNA-Seq Analysis of an Antisense Sequence Optimized for Exon Skipping in Duchenne Patients Reveals No Off-Target Effect by Domenger, Claire, Allais, Marine, François, Virginie, Léger, Adrien, Lecomte, Emilie, Montus, Marie, Servais, Laurent, Voit, Thomas, Moullier, Philippe, Audic, Yann, Le Guiner, Caroline

“…Non-coding uridine-rich small nuclear RNAs (UsnRNAs) have emerged in recent years as effective tools for exon skipping for the treatment of Duchenne muscular…”
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pycoQC, interactive quality control for Oxford Nanopore Sequencing by Leger, Adrien, Leonardi, Tommaso

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Accurate Identification and Quantification of DNA Species by Next-Generation Sequencing in Adeno-Associated Viral Vectors Produced in Insect Cells by Penaud-Budloo, Magalie, Lecomte, Emilie, Guy-Duché, Aurélien, Saleun, Sylvie, Roulet, Alain, Lopez-Roques, Céline, Tournaire, Benoît, Cogné, Benjamin, Léger, Adrien, Blouin, Véronique, Lindenbaum, Pierre, Moullier, Philippe, Ayuso, Eduard

“…Recombinant adeno-associated viral (rAAV) vectors have proven excellent tools for the treatment of many genetic diseases and other complex diseases. However,…”
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Single-Stranded DNA Virus Sequencing (SSV-Seq) for Characterization of Residual DNA and AAV Vector Genomes by Lecomte, Emilie, Leger, Adrien, Penaud-Budloo, Magalie, Ayuso, Eduard

“…With the success of clinical trials using recombinant adeno-associated viral vectors (rAAV), regulatory agencies ask for a more comprehensive characterization…”
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Adeno-associated viral vector-mediated transgene expression is independent of DNA methylation in primate liver and skeletal muscle by Léger, Adrien, Le Guiner, Caroline, Nickerson, Michael L, McGee Im, Kate, Ferry, Nicolas, Moullier, Philippe, Snyder, Richard O, Penaud-Budloo, Magalie

“…Recombinant adeno-associated viral (rAAV) vectors can support long-term transgene expression in quiescent tissues. Intramuscular (i.m.) administration of a…”
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Nanopore ReCappable sequencing maps SARS-CoV-2 5′ capping sites and provides new insights into the structure of sgRNAs by Ugolini, Camilla, Mulroney, Logan, Leger, Adrien, Castelli, Matteo, Criscuolo, Elena, Williamson, Maia Kavanagh, Davidson, Andrew D, Almuqrin, Abdulaziz, Giambruno, Roberto, Jain, Miten, Frigè, Gianmaria, Olsen, Hugh, Tzertzinis, George, Schildkraut, Ira, Wulf, Madalee G, Corrêa, Ivan R, Ettwiller, Laurence, Clementi, Nicola, Clementi, Massimo, Mancini, Nicasio, Birney, Ewan, Akeson, Mark, Nicassio, Francesco, Matthews, David A, Leonardi, Tommaso

“…Abstract The SARS-CoV-2 virus has a complex transcriptome characterised by multiple, nested subgenomic RNAsused to express structural and accessory proteins…”
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Short-lived recombinant adeno-associated virus transgene expression in dystrophic muscle is associated with oxidative damage to transgene mRNA by Dupont, Jean-Baptiste, Tournaire, Benoit, Georger, Christophe, Marolleau, Béatrice, Jeanson-Leh, Laurence, Ledevin, Mireille, Lindenbaum, Pierre, Lecomte, Emilie, Cogné, Benjamin, Dubreil, Laurence, Larcher, Thibaut, Gjata, Bernard, Van Wittenberghe, Laetitia, Le Guiner, Caroline, Penaud-Budloo, Magalie, Snyder, Richard O, Moullier, Philippe, Léger, Adrien

“…Preclinical gene therapy strategies using recombinant adeno-associated virus (AAV) vectors in animal models of Duchenne muscular dystrophy have shown dramatic…”
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Long-read sequencing of diagnosis and post-therapy medulloblastoma reveals complex rearrangement patterns and epigenetic signatures by Rausch, Tobias, Snajder, Rene, Leger, Adrien, Simovic, Milena, Giurgiu, Mădălina, Villacorta, Laura, Henssen, Anton G., Fröhling, Stefan, Stegle, Oliver, Birney, Ewan, Bonder, Marc Jan, Ernst, Aurelie, Korbel, Jan O.

“…Cancer genomes harbor a broad spectrum of structural variants (SVs) driving tumorigenesis, a relevant subset of which escape discovery using short-read…”
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NGS library preparation may generate artifactual integration sites of AAV vectors by Cogné, Benjamin, Snyder, Richard, Lindenbaum, Pierre, Dupont, Jean-Baptiste, Redon, Richard, Moullier, Philippe, Leger, Adrien

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Identification of miRNAs Involved in Reprogramming Acinar Cells into Insulin Producing Cells by Teichenne, Joan, Morró, Meritxell, Casellas, Alba, Jimenez, Veronica, Téllez i Besolí, Noèlia, Leger, Adrien, Bosch, Fatima, Ayuso, Eduard

“…Reprogramming acinar cells into insulin producing cells using adenoviral (Ad)-mediated delivery of Pdx1, Ngn3 and MafA (PNM) is an innovative approach for the…”
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