Search Results - "Lee, Nicholas Co"

Moving toward a higher efficiency of microcell-mediated chromosome transfer by Liskovykh, Mikhail, Lee, Nicholas Co, Larionov, Vladimir, Kouprina, Natalay

“…Microcell-mediated chromosome transfer (MMCT) technology enables individual mammalian chromosomes, megabase-sized chromosome fragments, or mammalian artificial…”
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Rapid generation of long tandem DNA repeat arrays by homologous recombination in yeast to study their function in mammalian genomes by Noskov, Vladimir N, Lee, Nicholas Co, Larionov, Vladimir, Kouprina, Natalay

“…We describe here a method to rapidly convert any desirable DNA fragment, as small as 100 bp, into long tandem DNA arrays up to 140 kb in size that are inserted…”
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Protecting a transgene expression from the HAC-based vector by different chromatin insulators by Lee, Nicholas CO, Kononenko, Artem V, Lee, Hee-Sheung, Tolkunova, Elena N, Liskovykh, Mikhail A, Masumoto, Hiroshi, Earnshaw, William C, Tomilin, Alexey N, Larionov, Vladimir, Kouprina, Natalay

“…Human artificial chromosomes (HACs) are vectors that offer advantages of capacity and stability for gene delivery and expression. Several studies have even…”
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Highly efficient CRISPR/Cas9-mediated TAR cloning of genes and chromosomal loci from complex genomes in yeast by Lee, Nicholas C O, Larionov, Vladimir, Kouprina, Natalay

“…Transformation-associated recombination (TAR) protocol allowing the selective isolation of full-length genes complete with their distal enhancer regions and…”
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A portable BRCA1-HAC (human artificial chromosome) module for analysis of BRCA1 tumor suppressor function by Kononenko, Artem V, Bansal, Ruchi, Lee, Nicholas C O, Grimes, Brenda R, Masumoto, Hiroshi, Earnshaw, William C, Larionov, Vladimir, Kouprina, Natalay

“…BRCA1 is involved in many disparate cellular functions, including DNA damage repair, cell-cycle checkpoint activation, gene transcriptional regulation, DNA…”
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Generation of a conditionally self-eliminating HAC gene delivery vector through incorporation of a tTA super(VP64) expression cassette by Kononenko, Artem V, Lee, Nicholas CO, Liskovykh, Mikhail, Masumoto, Hiroshi, Earnshaw, William C, Larionov, Vladimir, Kouprina, Natalay

“…Human artificial chromosome (HAC)-based vectors represent an alternative technology for gene delivery and expression with a potential to overcome the problems…”
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Effects of Anticancer Drug on Chromosome Instability (CIN) and New Clinical Implications for Tumor-Suppressing Therapies by Lee, Hee-Sheung, Lee, Nicholas CO, Kouprina, Natalay, Kim, Jung-Hyun, Kagansky, Alex, Bates, Susan, Trepel, Jane B., Pommier, Yves, Sackett, Dan, Larionov, Vladimir

“…Whole-chromosomal instability (CIN), manifested as unequal chromosome distribution during cell division, is a distinguishing feature of most cancer types. CIN…”
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A new assay for measuring chromosome instability (CIN) and identification of drugs that elevate CIN in cancer cells by Lee, Hee-Sheung, Lee, Nicholas C O, Grimes, Brenda R, Samoshkin, Alexander, Kononenko, Artem V, Bansal, Ruchi, Masumoto, Hiroshi, Earnshaw, William C, Kouprina, Natalay, Larionov, Vladimir

“…Aneuploidy is a feature of most cancer cells that is often accompanied by an elevated rate of chromosome mis-segregation termed chromosome instability (CIN)…”
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Exclusion of the 750-kb genetically unstable region at Xq27 as a candidate locus for prostate malignancy in HPCX1-linked families by Kouprina, Natalay, Lee, Nicholas C. O., Pavlicek, Adam, Samoshkin, Alexander, Kim, Jung-Hyun, Lee, Hee-Sheung, Varma, Sudhir, Reinhold, William C., Otstot, John, Solomon, Greg, Davis, Sean, Meltzer, Paul S., Schleutker, Johanna, Larionov, Vladimir

“…Several linkage studies provided evidence for the presence of the hereditary prostate cancer locus, HPCX1, at Xq27‐q28. The strongest linkage peak of prostate…”
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Site-specific recombination in Schizosaccharomyces pombe and systematic assembly of a 400kb transgene array in mammalian cells using the integrase of Streptomyces phage ϕBT1 by Xu, Zhengyao, Lee, Nicholas C. O., Dafhnis-Calas, Felix, Malla, Sunir, Smith, Margaret C. M., Brown, William R. A.

“…We have established the integrase of the Streptomyces phage ϕBT1 as a tool for eukaryotic genome manipulation. We show that the ϕBT1 integrase promotes…”
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Site-specific recombination in Schizosaccharomyces pombe and systematic assembly of a 400kb transgene array in mammalian cells using the integrase of Streptomyces phage {phi}BT1 by Xu, Zhengyao, Lee, Nicholas C.O, Dafhnis-Calas, Felix, Malla, Sunir, Smith, Margaret C.M, Brown, William R.A

“…We have established the integrase of the Streptomyces phage {phi}BT1 as a tool for eukaryotic genome manipulation. We show that the {phi}BT1 integrase promotes…”
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