Search Results - "Lee, Nicholas C. O."

Highly efficient CRISPR/Cas9-mediated TAR cloning of genes and chromosomal loci from complex genomes in yeast by Lee, Nicholas C O, Larionov, Vladimir, Kouprina, Natalay

“…Transformation-associated recombination (TAR) protocol allowing the selective isolation of full-length genes complete with their distal enhancer regions and…”
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Development of a novel HAC-based "gain of signal" quantitative assay for measuring chromosome instability (CIN) in cancer cells by Kim, Jung-Hyun, Lee, Hee-Sheung, Lee, Nicholas C O, Goncharov, Nikolay V, Kumeiko, Vadim, Masumoto, Hiroshi, Earnshaw, William C, Kouprina, Natalay, Larionov, Vladimir

“…Accumulating data indicates that chromosome instability (CIN) common to cancer cells can be used as a target for cancer therapy. At present the rate of…”
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A portable BRCA1-HAC (human artificial chromosome) module for analysis of BRCA1 tumor suppressor function by Kononenko, Artem V, Bansal, Ruchi, Lee, Nicholas C O, Grimes, Brenda R, Masumoto, Hiroshi, Earnshaw, William C, Larionov, Vladimir, Kouprina, Natalay

“…BRCA1 is involved in many disparate cellular functions, including DNA damage repair, cell-cycle checkpoint activation, gene transcriptional regulation, DNA…”
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Method to Assemble Genomic DNA Fragments or Genes on Human Artificial Chromosome with Regulated Kinetochore Using a Multi-Integrase System by Lee, Nicholas C. O, Kim, Jung-Hyun, Petrov, Nikolai S, Lee, Hee-Sheung, Masumoto, Hiroshi, Earnshaw, William C, Larionov, Vladimir, Kouprina, Natalay

“…The production of cells capable of carrying multiple transgenes to Mb-size genomic loci has multiple applications in biomedicine and biotechnology. In order to…”
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Kinetochore assembly and heterochromatin formation occur autonomously in Schizosaccharomyces pombe by Brown, William R. A., Thomas, Geraint, Lee, Nicholas C. O., Blythe, Martin, Liti, Gianni, Warringer, Jonas, Loose, Matthew W.

“…Kinetochores in multicellular eukaryotes are usually associated with heterochromatin. Whether this heterochromatin simply promotes the cohesion necessary for…”
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A new assay for measuring chromosome instability (CIN) and identification of drugs that elevate CIN in cancer cells by Lee, Hee-Sheung, Lee, Nicholas C O, Grimes, Brenda R, Samoshkin, Alexander, Kononenko, Artem V, Bansal, Ruchi, Masumoto, Hiroshi, Earnshaw, William C, Kouprina, Natalay, Larionov, Vladimir

“…Aneuploidy is a feature of most cancer cells that is often accompanied by an elevated rate of chromosome mis-segregation termed chromosome instability (CIN)…”
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Moving toward a higher efficiency of microcell-mediated chromosome transfer by Liskovykh, Mikhail, Lee, Nicholas Co, Larionov, Vladimir, Kouprina, Natalay

“…Microcell-mediated chromosome transfer (MMCT) technology enables individual mammalian chromosomes, megabase-sized chromosome fragments, or mammalian artificial…”
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Protecting a transgene expression from the HAC-based vector by different chromatin insulators by Lee, Nicholas CO, Kononenko, Artem V, Lee, Hee-Sheung, Tolkunova, Elena N, Liskovykh, Mikhail A, Masumoto, Hiroshi, Earnshaw, William C, Tomilin, Alexey N, Larionov, Vladimir, Kouprina, Natalay

“…Human artificial chromosomes (HACs) are vectors that offer advantages of capacity and stability for gene delivery and expression. Several studies have even…”
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Exclusion of the 750-kb genetically unstable region at Xq27 as a candidate locus for prostate malignancy in HPCX1-linked families by Kouprina, Natalay, Lee, Nicholas C. O., Pavlicek, Adam, Samoshkin, Alexander, Kim, Jung-Hyun, Lee, Hee-Sheung, Varma, Sudhir, Reinhold, William C., Otstot, John, Solomon, Greg, Davis, Sean, Meltzer, Paul S., Schleutker, Johanna, Larionov, Vladimir

“…Several linkage studies provided evidence for the presence of the hereditary prostate cancer locus, HPCX1, at Xq27‐q28. The strongest linkage peak of prostate…”
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Effects of Anticancer Drugs on Chromosome Instability and New Clinical Implications for Tumor-Suppressing Therapies by Lee, Hee-Sheung, Lee, Nicholas C O, Kouprina, Natalay, Kim, Jung-Hyun, Kagansky, Alex, Bates, Susan, Trepel, Jane B, Pommier, Yves, Sackett, Dan, Larionov, Vladimir

“…Whole chromosomal instability (CIN), manifested as unequal chromosome distribution during cell division, is a distinguishing feature of most cancer types. CIN…”
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Assembly of Multiple Full-Size Genes or Genomic DNA Fragments on Human Artificial Chromosomes Using the Iterative Integration System by Lee, Nicholas C O, Petrov, Nikolai S, Larionov, Vladimir, Kouprina, Natalay

“…Human artificial chromosomes (HACs) are gene delivery vectors that have been used for decades for gene functional studies. HACs have several advantages over…”
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Serine recombinases as tools for genome engineering by Brown, William R.A., Lee, Nicholas C.O., Xu, Zhengyao, Smith, Margaret C.M.

“…The serine recombinases differ mechanistically from the tyrosine recombinases and include proteins such as ϕC31 integrase which, unlike Cre and Flp, promote…”
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Re-engineering an alphoid(tetO)-HAC-based vector to enable high-throughput analyses of gene function by Kononenko, Artem V, Lee, Nicholas C O, Earnshaw, William C, Kouprina, Natalay, Larionov, Vladimir

“…Human artificial chromosome (HAC)-based vectors represent an alternative technology for gene delivery and expression with a potential to overcome the problems…”
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Site-specific recombination in Schizosaccharomyces pombe and systematic assembly of a 400kb transgene array in mammalian cells using the integrase of Streptomyces phage ϕBT1 by Xu, Zhengyao, Lee, Nicholas C. O., Dafhnis-Calas, Felix, Malla, Sunir, Smith, Margaret C. M., Brown, William R. A.

“…We have established the integrase of the Streptomyces phage ϕBT1 as a tool for eukaryotic genome manipulation. We show that the ϕBT1 integrase promotes…”
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Rapid generation of long tandem DNA repeat arrays by homologous recombination in yeast to study their function in mammalian genomes by Noskov, Vladimir N, Lee, Nicholas Co, Larionov, Vladimir, Kouprina, Natalay

“…We describe here a method to rapidly convert any desirable DNA fragment, as small as 100 bp, into long tandem DNA arrays up to 140 kb in size that are inserted…”
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From selective full-length genes isolation by TAR cloning in yeast to their expression from HAC vectors in human cells by Kouprina, Natalay, Lee, Nicholas C O, Kononenko, Artem V, Samoshkin, Alexander, Larionov, Vladimir

“…Transformation-associated recombination (TAR) cloning allows selective isolation of full-length genes and genomic loci as large circular Yeast Artificial…”
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Site-specific recombination in Schizosaccharomyces pombe and systematic assembly of a 400kb transgene array in mammalian cells using the integrase of Streptomyces phage {phi}BT1 by Xu, Zhengyao, Lee, Nicholas C.O, Dafhnis-Calas, Felix, Malla, Sunir, Smith, Margaret C.M, Brown, William R.A

“…We have established the integrase of the Streptomyces phage {phi}BT1 as a tool for eukaryotic genome manipulation. We show that the {phi}BT1 integrase promotes…”
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Re-engineering an alphoidtetO-HAC-based vector to enable high-throughput analyses of gene function by Kononenko, Artem V., Lee, Nicholas C. O., Earnshaw, William C., Kouprina, Natalay, Larionov, Vladimir

“…Human artificial chromosome (HAC)-based vectors represent an alternative technology for gene delivery and expression with a potential to overcome the problems…”
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Kinetochore assembly and heterochromatin formation occur autonomously in Schizosaccharomyces pombe by Brown, William R A, Thomas, Geraint, Lee, Nicholas C O, Blythe, Martin, Liti, Gianni, Warringer, Jonas, Loose, Matthew W

“…Kinetochores in multicellular eukaryotes are usually associated with heterochromatin. Whether this heterochromatin simply promotes the cohesion necessary for…”
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Analysis of the 9p21.3 sequence associated with coronary artery disease reveals a tendency for duplication in a CAD patient by Kouprina, Natalay, Liskovykh, Mikhail, Lee, Nicholas C O, Noskov, Vladimir N, Waterfall, Joshua J, Walker, Robert L, Meltzer, Paul S, Topol, Eric J, Larionov, Vladimir

“…Tandem segmental duplications (SDs) greater than 10 kb are widespread in complex genomes. They provide material for gene divergence and evolutionary…”
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