Search Results - "Lee, Nahum"
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Heterologous expression of tyrosinase (MelC2) from Streptomyces avermitilis MA4680 in E. coli and its application for ortho-hydroxylation of resveratrol to produce piceatannol
Published in Applied microbiology and biotechnology (01-10-2015)“…Recombinant tyrosinase from Streptomyces avermitilis MA4680, MelC2 (gi:499291317), was heterologously expressed in Escherichia coli BL21 (DE3). The expression…”
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Novel Sensor Platform Based on Aptamer-Conjugated Polypyrrole Nanotubes for Label-Free Electrochemical Protein Detection
Published in Chembiochem : a European journal of chemical biology (03-03-2008)“…We first present a simple yet versatile strategy for the functionalization of polymer nanotubes in a controlled fashion. Carboxylic-acid-functionalized…”
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Identification of the specific electron transfer proteins, ferredoxin, and ferredoxin reductase, for CYP105D7 in Streptomyces avermitilis MA4680
Published in Applied microbiology and biotechnology (01-06-2014)“…It was previously proposed that regiospecific hydroxylation of daidzein at 3′-position is mediated by cytochrome P450 hydroxylase (CYP105D7) in the presence of…”
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Regioselective hydroxylation of daidzein using P450 (CYP105D7) from Streptomyces avermitilis MA4680
Published in Biotechnology and bioengineering (01-03-2010)“…Regiospecific 3′‐hydroxylation reaction of daidzein was performed with CYP105D7 from Streptomyces avermitilis MA4680 expressed in Escherichia coli. The…”
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Screening of bacterial cytochrome P450s responsible for regiospecific hydroxylation of (iso)flavonoids
Published in Enzyme and microbial technology (07-04-2011)“…Screening of cytochrome P450 monoxygenases responsible for the regiospecific hydroxylation of flavones, isoflavones and chalcones was attempted using a P450…”
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Regio-specific hydroxylation of isoflavonoids using bacterial cytochrome P450s
Published in Journal of bioscience and bioengineering (01-11-2009)Get full text
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Regioselective Hydroxylation of trans-Resveratrol via Inhibition of Tyrosinase from Streptomyces avermitilis MA4680
Published in ACS chemical biology (19-10-2012)“…Secreted tyrosinase from melanin-forming Streptomyces avermitilis MA4680 was involved in both ortho-hydroxylation and further oxidation of trans-resveratrol,…”
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Effect of Extracellular Tyrosinase on the Expression Level of P450, Fpr, and Fdx and Ortho-hydroxylation of Daidzein in Streptomyces avermitilis
Published in Applied biochemistry and biotechnology (01-03-2018)“…We have reported that the expression of CYP105D7 in Streptomyces avermitilis produces 112.5 mg L −1 of 7,3′,4′-trihydroxyisoflavone (3'ODI) in 15 h of the…”
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Protein engineering of α2,3/2,6-sialyltransferase to improve the yield and productivity of in vitro sialyllactose synthesis
Published in Glycobiology (Oxford) (01-02-2014)“…In the large-quantity production of α2,3- and α2,6-sialyllactose (Neu5Ac(α2,3)Galβ1,4Glc (3'-SL) and Neu5Ac(α2,6)Galβ1,4Glc (6'-SL)) using sialyltransferases…”
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Rational design of modular allosteric aptamer sensor for label-free protein detection
Published in Biosensors & bioelectronics (15-01-2013)“…An aptamer can be redesigned to new functional molecules by conjugating with other oligonucleotides. However, it requires experimental trials to optimize the…”
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Bioconjugation of l-3,4-Dihydroxyphenylalanine Containing Protein with a Polysaccharide
Published in Bioconjugate chemistry (20-04-2011)“…We describe the simple bioconjugation strategy in combination of periodate chemistry and unnatural amino acid incorporation. The residue specific incorporation…”
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Integrating cell-free biosyntheses of heme prosthetic group and apoenzyme for the synthesis of functional P450 monooxygenase
Published in Biotechnology and bioengineering (01-04-2013)“…Harnessing the isolated protein synthesis machinery, cell‐free protein synthesis reproduces the cellular process of decoding genetic information in…”
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Biomolecular detection with a thin membrane transducer
Published in Lab on a chip (01-01-2008)“…We present a thin membrane transducer (TMT) that can detect nucleic acid based biomolecular reactions including DNA hybridization and protein recognition by…”
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Novel iron-sulfur containing NADPH-Reductase from Nocardia farcinica IFM10152 and fusion construction with CYP51 lanosterol demethylase
Published in Biotechnology and bioengineering (01-03-2012)“…CYP51, a sterol 14α‐demethylase, is one of the key enzymes involved in sterol biosynthesis and requires electrons transferred from its redox partners. A unique…”
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Analysis of recurrence in lung adenocarcinoma with spread through air spaces
Published in The Journal of thoracic and cardiovascular surgery (01-11-2023)“…Spread through air spaces is defined as tumor cells in air spaces away from the edge of tumor in lung carcinoma. It is associated with higher locoregional…”
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A-ring ortho-specific monohydroxylation of daidzein by cytochrome P450s of Nocardia farcinica IFM10152
Published in Biotechnology journal (01-11-2009)“…The bioconversion of the isoflavonoid daidzein using whole cell Nocardia farcinica IFM10152 showed two kinds of major metabolic modifications, i.e…”
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NORTHWEST TERRITORIES: An outbreak of anthrax
Published in Canadian veterinary journal (01-01-2007)Get full text
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Northwest Territories. An outbreak of anthrax (Bacillus anthracis) in free-roaming bison in the Northwest Territories, June-July 2006
Published in Canadian veterinary journal (01-01-2007)Get full text
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