Search Results - "Lee, Kelvin H"

The fickle CHO: a review of the causes, implications, and potential alleviation of the CHO cell line instability problem by Dahodwala, Hussain, Lee, Kelvin H

“…[Display omitted] •The CHO genome suffers from chromosomal rearrangements and other stability-related issues.•The CHO cell instability phenotype is…”
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A differentiating neural stem cell-derived astrocytic population mitigates the inflammatory effects of TNF-α and IL-6 in an iPSC-based blood-brain barrier model by Mantle, Jennifer L., Lee, Kelvin H.

“…Inflammation can be a risk factor for neurodegenerative diseases such as Alzheimer's disease (AD) and may also contribute to the progression of AD. Here, we…”
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High‐efficiency and multilocus targeted integration in CHO cells using CRISPR‐mediated donor nicking and DNA repair inhibitors by Hamaker, Nathaniel K., Lee, Kelvin H.

“…Efforts to leverage clustered regularly interspaced short palindromic repeats/CRISPR‐associated protein 9 (CRISPR/Cas9) for targeted genomic modifications in…”
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Biopharmaceutical Manufacturing: Historical Perspectives and Future Directions by Szkodny, Alana C, Lee, Kelvin H

“…This review describes key milestones related to the production of biopharmaceuticals-therapies manufactured using recombinant DNA technology. The market for…”
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Knockout of a difficult‐to‐remove CHO host cell protein, lipoprotein lipase, for improved polysorbate stability in monoclonal antibody formulations by Chiu, Josephine, Valente, Kristin N., Levy, Nicholas E., Min, Lie, Lenhoff, Abraham M., Lee, Kelvin H.

“…ABSTRACT While the majority of host cell protein (HCP) impurities are effectively removed in typical downstream purification processes, a small population of…”
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Tuning and Predicting Mesh Size and Protein Release from Step Growth Hydrogels by Rehmann, Matthew S, Skeens, Kelsi M, Kharkar, Prathamesh M, Ford, Eden M, Maverakis, Emanual, Lee, Kelvin H, Kloxin, April M

“…Hydrogel-based depots are of growing interest for release of biopharmaceuticals; however, a priori selection of hydrogel compositions that will retain proteins…”
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Antibody transcytosis across brain endothelial-like cells occurs nonspecifically and independent of FcRn by Ruano-Salguero, John S., Lee, Kelvin H.

“…The blood-brain barrier (BBB) hinders the brain delivery of therapeutic immunoglobulin γ (IgG) antibodies. Evidence suggests that IgG-specific processing…”
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Chromosome‐scale scaffolds for the Chinese hamster reference genome assembly to facilitate the study of the CHO epigenome by Hilliard, William, MacDonald, Madolyn L., Lee, Kelvin H.

“…The Chinese hamster genome serves as a reference genome for the study of Chinese hamster ovary (CHO) cells, the preferred host system for biopharmaceutical…”
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Minimum Transendothelial Electrical Resistance Thresholds for the Study of Small and Large Molecule Drug Transport in a Human in Vitro Blood–Brain Barrier Model by Mantle, Jennifer L, Min, Lie, Lee, Kelvin H

“…A human cell-based in vitro model that can accurately predict drug penetration into the brain as well as metrics to assess these in vitro models are valuable…”
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Applications of proteomic methods for CHO host cell protein characterization in biopharmaceutical manufacturing by Valente, Kristin N, Levy, Nicholas E, Lee, Kelvin H, Lenhoff, Abraham M

“…Chinese hamster ovary (CHO) cells are the most prevalent host organism for production of recombinant therapeutic proteins, including monoclonal antibodies…”
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EvalDNA: a machine learning-based tool for the comprehensive evaluation of mammalian genome assembly quality by MacDonald, Madolyn L, Lee, Kelvin H

“…To select the most complete, continuous, and accurate assembly for an organism of interest, comprehensive quality assessment of assemblies is necessary. We…”
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Biolayer interferometry for adeno-associated virus capsid titer measurement and applications to upstream and downstream process development by Leibiger, Thomas M., Remmler, Luke A., Green, Erica A., Lee, Kelvin H.

“…Faster and more accurate analytical methods are needed to support the advancement of recombinant adeno-associated virus (rAAV) production systems. Recently,…”
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Host cell protein impurities in chromatographic polishing steps for monoclonal antibody purification by Levy, Nicholas E., Valente, Kristin N., Lee, Kelvin H., Lenhoff, Abraham M.

“…ABSTRACT Downstream purification of monoclonal antibodies (mAbs) is normally performed using a platform process that is empirically tuned to optimize impurity…”
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The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line by Palsson, Bernhard O, Wang, Jun, Xu, Xun, Nagarajan, Harish, Lewis, Nathan E, Pan, Shengkai, Cai, Zhiming, Liu, Xin, Chen, Wenbin, Xie, Min, Wang, Wenliang, Hammond, Stephanie, Andersen, Mikael R, Neff, Norma, Passarelli, Benedetto, Koh, Winston, Fan, H Christina, Wang, Jianbin, Gui, Yaoting, Lee, Kelvin H, Betenbaugh, Michael J, Quake, Stephen R, Famili, Iman

“…Since 1987, immortalized cells from the ovary of a Chinese hamster have been the workhorse for producing recombinant therapeutics, including monoclonal…”
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A reference genome of the Chinese hamster based on a hybrid assembly strategy by Rupp, Oliver, MacDonald, Madolyn L., Li, Shangzhong, Dhiman, Heena, Polson, Shawn, Griep, Sven, Heffner, Kelley, Hernandez, Inmaculada, Brinkrolf, Karina, Jadhav, Vaibhav, Samoudi, Mojtaba, Hao, Haiping, Kingham, Brewster, Goesmann, Alexander, Betenbaugh, Michael J., Lewis, Nathan E., Borth, Nicole, Lee, Kelvin H.

“…Accurate and complete genome sequences are essential in biotechnology to facilitate genome‐based cell engineering efforts. The current genome assemblies for…”
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Analytical characterization of host‐cell‐protein‐rich aggregates in monoclonal antibody solutions by Herman, Chase E., Min, Lie, Choe, Leila H., Maurer, Ronald W., Xu, Xuankuo, Ghose, Sanchayita, Lee, Kelvin H., Lenhoff, Abraham M.

“…Host‐cell proteins (HCPs) and high molecular weight (HMW) species have historically been treated as independent classes of impurities in the downstream…”
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Membrane configuration optimization for a murine in vitro blood–brain barrier model by Wuest, Diane M., Wing, Allison M., Lee, Kelvin H.

“…► A comprehensive screening study identifies optimal in vitro BBB membrane set points. ► Pore diameter, material, manufacturer and co-culture orientation are…”
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Fabrication of 3D Biomimetic Microfluidic Networks in Hydrogels by Heintz, Keely A., Bregenzer, Michael E., Mantle, Jennifer L., Lee, Kelvin H., West, Jennifer L., Slater, John H.

“…A laser‐based hydrogel degradation technique is developed that allows for local control over hydrogel porosity, fabrication of 3D vascular‐derived, biomimetic,…”
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Restoration of DNA repair mitigates genome instability and increases productivity of Chinese hamster ovary cells by Spahn, Philipp N., Zhang, Xiaolin, Hu, Qing, Lu, Huiming, Hamaker, Nathaniel K., Hefzi, Hooman, Li, Shangzhong, Kuo, Chih‐Chung, Huang, Yingxiang, Lee, Jamie C., Davis, Anthony J., Ly, Peter, Lee, Kelvin H., Lewis, Nathan E.

“…Chinese hamster ovary (CHO) cells are the primary host for manufacturing of therapeutic proteins. However, productivity loss is a major problem and is…”
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Comparison of vector elements and process conditions in transient and stable suspension HEK293 platforms using SARS-CoV-2 receptor binding domain as a model protein by Green, Erica A, Hamaker, Nathaniel K, Lee, Kelvin H

“…Mammalian cell lines are frequently used as protein expression hosts because of their ability to correctly fold and assemble complex proteins, produce them at…”
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