Contribution of the Ogawa-Kudoh swab culture method to the diagnosis of pulmonary tuberculosis in Brazil

OBJECTIVE: To analyse the contribution of the Ogawa-Kudoh (O-K) swab culture method to the diagnosis of pulmonary tuberculosis (PTB) in four different regions of Brazil.DESIGN: This study was carried out in two phases. Phase 1 was designed to compare the direct swab culture method (O-K) with the cul...

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Bibliographic Details
Published in:	The international journal of tuberculosis and lung disease Vol. 17; no. 6; pp. 782 - 786
Main Authors:	Palaci, M., Peres, R. L., Maia, R., Cunha, E. A. T., Ribeiro, M. O., Lecco, R., de Souza Ribeiro, C., Ferro e Silva, R. R., Vinhas, S. A., Dietze, R., Vianna, S., de Morais, C. G. V.
Format:	Journal Article
Language:	English
Published:	Paris, France International Union Against Tuberculosis and Lung Disease 01-06-2013 International Union against Tuberculosis and Lung Disease
Subjects:	Acetylcysteine - chemistry Adolescent Adult Aged Aged, 80 and over Bacilli Bacterial diseases Bacterial diseases of the respiratory system Bacteriological Techniques - methods Biological and medical sciences Brazil - epidemiology Culture Diagnosis Human bacterial diseases Humans Infectious diseases Medical sciences Middle Aged Mycobacterium Ogawa-Kudoh Swab Method Pneumology Prospective Studies Retrospective Studies Sensitivity and Specificity Sodium Hydroxide - chemistry Sputum - microbiology Tuberculosis Tuberculosis and atypical mycobacterial infections Tuberculosis, Pulmonary - diagnosis Tuberculosis, Pulmonary - epidemiology Young Adult South America Brazil America Lung disease Methodology Respiratory disease Samplings Pulmonary tuberculosis tuberculosis Mycobacterial infection Method Infection Bacteriosis Cultural aspect Diagnosis Technique Culture Ogawa-Kudoh swab method
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Summary:	OBJECTIVE: To analyse the contribution of the Ogawa-Kudoh (O-K) swab culture method to the diagnosis of pulmonary tuberculosis (PTB) in four different regions of Brazil.DESIGN: This study was carried out in two phases. Phase 1 was designed to compare the direct swab culture method (O-K) with the culture concentrated method (N-acetyl-L-cysteine-sodium hydroxide [NALC-NaOH]); for this purpose, 569 sputum samples were cultured by both methods. Phase 2 was carried out to assess the contribution of the O-K method to the diagnosis of PTB in four different regions in Brazil, based on the evaluation of 19 163 sputum samples.RESULTS: In the first phase of the study, O-K culture had a sensitivity of 94.8% and specificity of 99.8% in cases confirmed by NALC-NaOH/Löwenstein-Jensen (LJ) culture. In the second phase of the study, the overall contribution of O-K culture compared to acid-fast bacilli (AFB) examination (AFB−/culture+) to the diagnosis of PTB was 29.8%.CONCLUSION: O-K culture contributes significantly to the diagnosis of smear-negative PTB. Importantly, this method allows the recovery of clinical isolates in areas where use of the standard culture centrifuge is impossible, indicating that the O-K swab culture method should become a standard method for TB diagnosis in these regions.
Bibliography:	(R) Medicine - General 1027-3719(20130601)17:6L.782;1- ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	1027-3719 1815-7920
DOI:	10.5588/ijtld.12.0500