A glycoprotein with anti-inflammatory properties secreted by an Aspergillus nidulans modified strain

Total RNA from lipopolysaccharide (LPS)-stimulated rat macrophages used to treat protoplasts from an Aspergillus nidulans strain originated the RT2 regenerated strain, whose culture supernatant showed anti-inflammatory activity in Wistar rats. The protein fraction presenting such anti-inflammatory a...

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Bibliographic Details
Published in:	The journal of venomous animals and toxins including tropical diseases Vol. 13; no. 2; pp. 527 - 543
Main Authors:	Queiroz, J. C. F.(University of São Paulo Institute of Biomedical Sciences Department of Parasitology and Biotechnology Research Center), Teixeira, C. F. P.(Butantan Institute Laboratory of Pharmacology), Lebrun, I.(Butantan Institute Biochemistry and Biophysics Laboratorty), Spencer, P. J.(Nuclear Energy Research Institute IVBiotechnology Center), Zucchi, T. M. A. D.(University of São Paulo Institute of Biomedical Sciences Department of Parasitology and Biotechnology Research Center)
Format:	Journal Article
Language:	English
Published:	Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP 2007 Centro de Estudos de Venenos e Animais Peçonhentos SciELO
Subjects:	anti-inflammatory activity Aspergillus nidulans glycoprotein protein purification and characterization TOXICOLOGY TROPICAL MEDICINE glycoprotein Aspergillus nidulans protein purification and characterization anti-inflammatory activity
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Summary:	Total RNA from lipopolysaccharide (LPS)-stimulated rat macrophages used to treat protoplasts from an Aspergillus nidulans strain originated the RT2 regenerated strain, whose culture supernatant showed anti-inflammatory activity in Wistar rats. The protein fraction presenting such anti-inflammatory activity was purified and biochemically identified. The screening of the fraction responsible for such anti-inflammatory property was performed by evaluating the inhibition of carrageenan-induced paw edema in male Swiss mice. Biochemical analyses of the anti-inflammatory protein used chromatography, carbohydrates quantification of the protein sample, amino acids content analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Total sugar quantification revealed 32% glycosylation of the protein fraction. Amino acid analysis of such fraction showed a peculiar pattern presenting 29% valine. SDS-PAGE revealed that the protein sample is pure and its molecular weight is about 40kDa. Intravenous injection of the isolated substance into mice significantly inhibited carrageenan-induced paw edema. The isolated glycoprotein decreased carrageenan-induced paw edema in a prostaglandin-dependent phase, suggesting an inhibitory effect of the isolated glycoprotein on prostaglandin synthesis.
Bibliography:	10.1590/S1678-91992007000200010 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000200010 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1678-9199 1678-9199
DOI:	10.1590/s1678-91992007000200010