A One-Bead One-Peptide Combinatorial Library Method for B-Cell Epitope Mapping

The one-bead one-peptide combinatorial library method represents a powerful approach to the discovery of binding peptides for various macromolecular targets. It involves the synthesis of millions of peptides on beads such that each bead displays only one peptide entity. The peptide–beads that intera...

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Bibliographic Details
Published in:	Methods (San Diego, Calif.) Vol. 9; no. 3; pp. 482 - 493
Main Authors:	Lam, Kit S., Lake, Douglas, Salmon, Sydney E., Smith, John, Chen, Man-Ling, Wade, Shelly, Abdul-Latif, Farid, Knapp, Richard J., Leblova, Zuzana, Ferguson, Ronald D., Krchnak, Viktor, Sepetov, Nikolai F., Lebl, Michal
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01-06-1996
Online Access:	Get full text
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Summary:	The one-bead one-peptide combinatorial library method represents a powerful approach to the discovery of binding peptides for various macromolecular targets. It involves the synthesis of millions of peptides on beads such that each bead displays only one peptide entity. The peptide–beads that interact with a specific macromolecular target are then isolated for structure determination. We have applied this method to discovering peptide ligands for several murine monoclonal antibodies: (i) anti-β-endorphin (continuous epitope), (ii) anti-vmos peptide, (iii) anti-human insulin (discontinuous epitope), and (iv) surface immunoglobulins (μκ) of two murine B-cell lymphoma cell lines (antigen unknown).
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1046-2023 1095-9130
DOI:	10.1006/meth.1996.0056