Initiation of spontaneous tumors in radish (Raphanus sativus): Cellular, molecular and physiological events

In plant meristems, the balance of cell proliferation and differentiation is maintained by phytohormones, specifically auxin and cytokinin, as well as transcription factors. Changing of the cytokinin/auxin balance in plants may lead to developmental abnormalities, and in particular, to the formation...

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Published in:	Journal of plant physiology Vol. 173; pp. 97 - 104
Main Authors:	Lebedeva (Osipova), Maria A., Tvorogova, Varvara E., Vinogradova, Alena P., Gancheva, Maria S., Azarakhsh, Mahboobeh, Ilina, Elena L., Demchenko, Kirill N., Dodueva, Irina E., Lutova, Lyudmila A.
Format:	Journal Article
Language:	English
Published:	Germany Elsevier GmbH 15-01-2015
Subjects:	Abnormalities Amino Acid Sequence Auxin Balancing Cambium Cambium - cytology Cambium - genetics Cambium - physiology Cell Differentiation Cell Proliferation Cytokinins - metabolism Formations Gene expression Gene Expression Regulation, Plant Genes Genes, Reporter Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Indoleacetic Acids - metabolism Meristem - cytology Meristem - genetics Meristem - physiology Plant Growth Regulators - metabolism Plant Proteins - genetics Plant Proteins - metabolism Plant Roots - cytology Plant Roots - genetics Plant Roots - physiology Plant Tumors Promoter Regions, Genetic - genetics Radish Radishes Raphanus - cytology Raphanus - genetics Raphanus - physiology Roots Sequence Alignment Spontaneous Tumors WOX5 Plant tumors Radish Auxin Cambium WOX5
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Summary:	In plant meristems, the balance of cell proliferation and differentiation is maintained by phytohormones, specifically auxin and cytokinin, as well as transcription factors. Changing of the cytokinin/auxin balance in plants may lead to developmental abnormalities, and in particular, to the formation of tumors. The examples of spontaneous tumor formation in plants include tumors formed on the roots of radish (Raphanus sativus) inbred lines. Previously, it was found that the cytokinin/auxin ratio is altered in radish tumors. In this study, a detailed histological analysis of spontaneous radish tumors was performed, revealing a possible mechanism of tumor formation, namely abnormal cambial activity. The analysis of cell proliferation patterns revealed meristematic foci in radish tumors. By using a fusion of an auxin-responsive promoter (DR5) and a reporter gene, the involvement of auxin in developmental processes in tumors was shown. In addition, the expression of the root meristem-specific WUSCHEL-related homeobox 5 (WOX5) gene was observed in cells adjacent to meristematic foci. Taken together, the results of the present study show that tumor tissues share some characteristics with root apical meristems, including the presence of auxin-response maxima in meristematic foci with adjacent cells expressing WOX5.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0176-1617 1618-1328
DOI:	10.1016/j.jplph.2014.07.030