Impact of the presence of debris on semen analysis using an automated system (SQA-V)

[LANGUAGE= "English"] INTRODUCTION: This study examined the influence of the manual assessment of debris on the results of sperm concentration, progressive motility, and normal morphology measured using the SQA-V system (Medical Electronic Systems LLC, Los Angeles, CA, USA).METHODS: Sixty...

Full description

Saved in:

Bibliographic Details
Published in:	International journal of medical biochemistry Vol. 4; no. 3; p. 211
Main Authors:	Ilardo, Claudio, Ostengo, Violaine, Leatitia Eon, Trentin, Julie, Gilles Regnier Vigouroux, Quere, Guillaune, Sanguinet, Pierre
Format:	Journal Article
Language:	English
Published:	Istanbul Kare Publishing 01-01-2021
Subjects:	Morphology Motility Regression analysis Sperm Los Angeles California United States > US
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	[LANGUAGE= "English"] INTRODUCTION: This study examined the influence of the manual assessment of debris on the results of sperm concentration, progressive motility, and normal morphology measured using the SQA-V system (Medical Electronic Systems LLC, Los Angeles, CA, USA).METHODS: Sixty samples were analyzed simultaneously and independently by different operators using the manual technique and the SQA-V analyzer. Three measurements (sperm concentration, progressive motility, and normal morphology) of each sample were assessed using the 4 debris assessment levels: none/few, moderate, many, and gross.RESULTS: Given an optimal assessment of debris, the study data indicate that the SQA-V provided results that had very good agreement with those of the manual method: sperm concentration (rho=0.987, regression analysis formula: y=0.961x+1.962, p<0.0001), progressive motility (rho=0.949, regression analysis formula: y=0.989x+0.418, p<0.0001), and normal morphology (rho=0.694, regression analysis formula: y=0.678x+4.061, p<0.0001). Underestimation of debris increased sperm concentration and decreased motility and normal morphology, while overestimation of debris decreased sperm concentration and increased motility and normal morphology.DISCUSSION AND CONCLUSION: The results indicated that the performance of the SQA-V remains subject to the competency of the operator.
ISSN:	2587-2362 2618-642X
DOI:	10.14744/ijmb.2021.97658