Search Results - "Leal, Nicole A"

Ribonucleosides for an Artificially Expanded Genetic Information System by Kim, Hyo-Joong, Leal, Nicole A, Hoshika, Shuichi, Benner, Steven A

“…Rearranging hydrogen bonding groups adds nucleobases to an artificially expanded genetic information system (AEGIS), pairing orthogonally to standard…”
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Artificial Genetic Systems: Self-Avoiding DNA in PCR and Multiplexed PCR by Hoshika, Shuichi, Chen, Fei, Leal, Nicole A, Benner, Steven A

“…Discerning in their choice of partner: The DNA of a self‐avoiding molecular‐recognition system (SAMRS) binds to natural DNA but not to members of the same…”
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Reconstructed evolutionary adaptive paths give polymerases accepting reversible terminators for sequencing and SNP detection by Chen, Fei, Gaucher, Eric A, Leal, Nicole A, Hutter, Daniel, Havemann, Stephanie A, Govindarajan, Sridhar, Ortlund, Eric A, Benner, Steven A

“…Any system, natural or human-made, is better understood if we analyze both its history and its structure. Here we combine structural analyses with a…”
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Hachimoji DNA and RNA: A genetic system with eight building blocks by Hoshika, Shuichi, Leal, Nicole A, Kim, Myong-Jung, Kim, Myong-Sang, Karalkar, Nilesh B, Kim, Hyo-Joong, Bates, Alison M, Watkins, Jr, Norman E, SantaLucia, Holly A, Meyer, Adam J, DasGupta, Saurja, Piccirilli, Joseph A, Ellington, Andrew D, SantaLucia, Jr, John, Georgiadis, Millie M, Benner, Steven A

“…We report DNA- and RNA-like systems built from eight nucleotide "letters" (hence the name "hachimoji") that form four orthogonal pairs. These synthetic systems…”
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Human ATP:Cob(I)alamin Adenosyltransferase and Its Interaction with Methionine Synthase Reductase by Leal, Nicole A, Olteanu, Horatiu, Banerjee, Ruma, Bobik, Thomas A

“…The final step in the conversion of vitamin B 12 into coenzyme B 12 (adenosylcobalamin, AdoCbl) is catalyzed by ATP:cob(I)alamin adenosyltransferase (ATR)…”
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Directed Evolution of Polymerases To Accept Nucleotides with Nonstandard Hydrogen Bond Patterns by Laos, Roberto, Shaw, Ryan, Leal, Nicole A, Gaucher, Eric, Benner, Steven

“…Artificial genetic systems have been developed by synthetic biologists over the past two decades to include additional nucleotides that form additional…”
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Identification of the Human and Bovine ATP:Cob(I)alamin Adenosyltransferase cDNAs Based on Complementation of a Bacterial Mutant by Leal, Nicole A, Park, Sanghee D, Kima, Peter E, Bobik, Thomas A

“…In humans, deficiencies in coenzyme B12-dependent methylmalonyl-CoA mutase (MCM) lead to methylmalonyl aciduria, a rare disease that is often fatal in…”
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“Skinny” and “Fat” DNA: Two New Double Helices by Hoshika, Shuichi, Singh, Isha, Switzer, Christopher, Molt, Robert W, Leal, Nicole A, Kim, Myong-Jung, Kim, Myong-Sang, Kim, Hyo-Joong, Georgiadis, Millie M, Benner, Steven A

“…According to the iconic model, the Watson–Crick double helix exploits nucleobase pairs that are both size complementary (big purines pair with small…”
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Conversion Strategy Using an Expanded Genetic Alphabet to Assay Nucleic Acids by Yang, Zunyi, Durante, Michael, Glushakova, Lyudmyla G, Sharma, Nidhi, Leal, Nicole A, Bradley, Kevin M, Chen, Fei, Benner, Steven A

“…Methods to detect DNA and RNA (collectively xNA) are easily plagued by noise, false positives, and false negatives, especially with increasing levels of…”
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Transcription, Reverse Transcription, and Analysis of RNA Containing Artificial Genetic Components by Leal, Nicole A, Kim, Hyo-Joong, Hoshika, Shuichi, Kim, Myong-Jung, Carrigan, Matthew A, Benner, Steven A

“…Expanding the synthetic biology of artificially expanded genetic information systems (AEGIS) requires tools to make and analyze RNA molecules having added…”
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PduL Is an Evolutionarily Distinct Phosphotransacylase Involved in B12-Dependent 1,2-Propanediol Degradation by Salmonella enterica Serovar Typhimurium LT2 by YU LIU, LEAF, Nicole A, SAMPSON, Edith M, JOHNSON, Celeste L. V, HAVEMANN, Gregory D, BOBIK, Thomas A

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Synthesis and Enzymology of 2′-Deoxy-7-deazaisoguanosine Triphosphate and Its Complement: A Second Generation Pair in an Artificially Expanded Genetic Information System by Karalkar, Nilesh B, Leal, Nicole A, Kim, Myong-Sang, Bradley, Kevin M, Benner, Steven A

“…As with natural nucleic acids, pairing between artificial nucleotides can be influenced by tautomerism, with different placements of protons on the…”
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2'-Deoxy-1-methylpseudocytidine, a stable analog of 2'-deoxy-5-methylisocytidine by KIM, Hyo-Joong, LEAL, Nicole A, BENNER, Steven A

“…2'-deoxy-5-methylisocytidine is widely used in assays to personalize the care of patients infected with HIV, hepatitis C, and other infectious agents. However,…”
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PduP is a coenzyme-a-acylating propionaldehyde dehydrogenase associated with the polyhedral bodies involved in B12-dependent 1,2-propanediol degradation by Salmonella enterica serovar typhimurium LT2 by LEAL, Nicole A, HAVEMANN, Gregory D, BOBIK, Thomas A

“…Salmonella enterica forms polyhedral bodies involved in coenzyme-B12-dependent 1,2-propanediol degradation. Prior studies showed that these bodies consist of a…”
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Dynamic assembly of primers on nucleic acid templates by Leal, Nicole A, Sukeda, Makoto, Benner, Steven A

“…A strategy is presented that uses dynamic equlibria to assemble in situ composite DNA polymerase primers, having lengths of 14 or 16 nt, from DNA fragments…”
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Labeled Nucleoside Triphosphates with Reversibly Terminating Aminoalkoxyl Groups by Hutter, Daniel, Kim, Myong-Jung, Karalkar, Nilesh, Leal, Nicole A., Chen, Fei, Guggenheim, Evan, Visalakshi, Visa, Olejnik, Jerzy, Gordon, Steven, Benner, Steven A.

“…Nucleoside triphosphates having a 3′-ONH 2 blocking group have been prepared with and without fluorescent tags on their nucleobases. DNA polymerases were…”
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Functional Genomic, Biochemical, and Genetic Characterization of the Salmonella pduO Gene, an ATP:Cob(I)alamin Adenosyltransferase Gene by Johnson, C L, Pechonick, E, Park, S D, Havemann, G D, Leal, N A, Bobik, T A

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In vivo expression of human ATP:cob(I)alamin adenosyltransferase (ATR) using recombinant adeno-associated virus (rAAV) serotypes 2 and 8 by Erger, Kirsten E., Conlon, Thomas J., Leal, Nicole A., Zori, Robert, Bobik, Thomas A., Flotte, Terence R.

“…Background Methylmalonic aciduria (MMA) is an autosomal recessive disease with symptoms that include ketoacidosis, lethargy, recurrent vomiting, dehydration,…”
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Self-Avoiding molecular Recognition Systems (SAMRS) by Hoshika, Shuichi, Chen, Fei, Leal, Nicole A., Benner, Steven A.

“…Abstract Reported here is a "Self-Avoiding Molecular Recognition Systems" (SAMRS), a species of DNA that can bind via simple rules to natural DNA but cannot…”
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PduL Is an Evolutionarily Distinct Phosphotransacylase Involved in B 12 -Dependent 1,2-Propanediol Degradation by Salmonella enterica Serovar Typhimurium LT2 by Liu, Yu, Leal, Nicole A., Sampson, Edith M., Johnson, Celeste L. V., Havemann, Gregory D., Bobik, Thomas A.

“…Salmonella enterica degrades 1,2-propanediol (1,2-PD) in a coenzyme B 12 -dependent manner. Previous enzymatic assays of crude cell extracts indicated that a…”
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