Cashew gum protects the intestinal mucosa from morphological changes promoted by the aggression of 5‐Fluorouracil
Intestinal mucositis is a frequent complication in the treatment of cancer with the chemotherapeutic agent 5‐fluorouracil (5‐FU). So far there is still no effective treatment of this condition. Cashew gum (CG) has been reported as a potent anti‐inflammatory. The present study aimed to evaluate the e...
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| Published in: | The FASEB journal Vol. 33; no. S1; p. 767.33 |
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| Main Authors: | , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
The Federation of American Societies for Experimental Biology
01-04-2019
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Intestinal mucositis is a frequent complication in the treatment of cancer with the chemotherapeutic agent 5‐fluorouracil (5‐FU). So far there is still no effective treatment of this condition. Cashew gum (CG) has been reported as a potent anti‐inflammatory. The present study aimed to evaluate the effect of Cashew gum (Anacardium occidentale L.) on 5‐fluorouracil‐induced intestinal mucositis in Swiss mice. The mice (25–30g) were secreted into 5 groups (n = 6): Saline group (NaCl 0.9%), 5‐FU group, CG group (90mg/kg CG, vo), CLX group (Celecoxib ip), CLX + CG group (Celecoxib ip, 90mg/kg GC). All animals except Saline group received 5‐FU at the concentration of 450mg/kg in the first experimental protocol. Duodenal portions were removed for evaluation of mucositis by histopathological and immunohistochemical analysis for cyclooxygenase‐2 (COX‐2). The results of the histopathological analysis demonstrated that 5‐FU promoted structural alterations of the intestinal mucosa, evidenced by the reduction of villi height, deepening of the crypts, and intense inflammatory infiltrate, in comparison to the Salina group, characterized by the integrity of the villi and crypts and absence of inflammatory infiltrate. CG animals showed a significant reversal of the effects promoted by antineoplastic in the villi, crypts and inflammatory cells infiltration in comparison to the 5‐FU group. CLX group, as well as CLX + GC, were able to significantly prevent (p <0.05) destruction of crypts, shortening of villi with cellular vacuolization, and inflammatory infiltrate. Regarding the immunoblotting for expression of COX‐2, it can be observed that the saline group showed subtle immunostaining when compared to the 5‐FU group (p <0.05), in which intense and generalized immunostaining was observed. In the CG, CLX and CLX + CG groups, there was a decrease in the immunostaining when compared to the 5‐FU lesion group. In addition, the amount of COX‐2‐immunolabelled cells in the CLX + CG group was lower than the CG treated group. It is concluded that CG prevented the intestinal mucosa promoted by the 5‐FU antineoplastic, in addition to decreasing the expression of the COX‐2 enzyme, an important element in the onset and exacerbation of inflammation.
Support or Funding Information
This study received funding of CNPQ and CAPES
This is from the Experimental Biology 2019 Meeting. There is no full text article associated with this published in The FASEB Journal. |
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| ISSN: | 0892-6638 1530-6860 |
| DOI: | 10.1096/fasebj.2019.33.1_supplement.767.33 |